Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1353 and fliR are separated by 4 nucleotides fliR and flhB are separated by 17 nucleotides flhB and flhA are separated by 118 nucleotides
Shewana3_1353: Shewana3_1353 - flagellar biosynthetic protein FliQ (RefSeq), at 1,565,520 to 1,565,789
_1353
Shewana3_1354: fliR - flagellar biosynthesis protein FliR (RefSeq), at 1,565,794 to 1,566,591
fliR
Shewana3_1355: flhB - flagellar biosynthesis protein FlhB (RefSeq), at 1,566,609 to 1,567,739
flhB
Shewana3_1356: flhA - flagellar biosynthesis protein FlhA (RefSeq), at 1,567,858 to 1,569,957
flhA
Position (kb)
1566
1567
1568 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1565.833 kb on + strand at 1565.923 kb on + strand, within fliR at 1565.969 kb on + strand, within fliR at 1566.111 kb on - strand, within fliR at 1566.222 kb on + strand, within fliR at 1566.294 kb on - strand, within fliR at 1566.330 kb on - strand, within fliR at 1566.340 kb on + strand, within fliR at 1566.741 kb on + strand, within flhB at 1566.834 kb on + strand, within flhB at 1566.834 kb on + strand, within flhB at 1566.842 kb on - strand, within flhB at 1567.313 kb on + strand, within flhB at 1567.415 kb on + strand, within flhB at 1567.429 kb on - strand, within flhB at 1567.478 kb on + strand, within flhB at 1567.605 kb on + strand, within flhB at 1567.681 kb on + strand at 1567.702 kb on - strand at 1567.881 kb on - strand at 1567.882 kb on - strand at 1567.939 kb on - strand at 1568.056 kb on + strand at 1568.064 kb on - strand at 1568.064 kb on - strand at 1568.064 kb on - strand at 1568.064 kb on - strand at 1568.426 kb on + strand, within flhA at 1568.505 kb on + strand, within flhA at 1568.549 kb on + strand, within flhA
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,565,833 + +0.3 1,565,923 + fliR Shewana3_1354 0.16 +2.3 1,565,969 + fliR Shewana3_1354 0.22 -3.3 1,566,111 - fliR Shewana3_1354 0.40 +1.3 1,566,222 + fliR Shewana3_1354 0.54 -2.4 1,566,294 - fliR Shewana3_1354 0.63 +1.3 1,566,330 - fliR Shewana3_1354 0.67 +0.4 1,566,340 + fliR Shewana3_1354 0.68 -2.9 1,566,741 + flhB Shewana3_1355 0.12 -2.4 1,566,834 + flhB Shewana3_1355 0.20 -1.9 1,566,834 + flhB Shewana3_1355 0.20 +1.3 1,566,842 - flhB Shewana3_1355 0.21 -1.6 1,567,313 + flhB Shewana3_1355 0.62 -0.9 1,567,415 + flhB Shewana3_1355 0.71 -0.3 1,567,429 - flhB Shewana3_1355 0.73 +0.4 1,567,478 + flhB Shewana3_1355 0.77 -1.3 1,567,605 + flhB Shewana3_1355 0.88 -1.0 1,567,681 + +0.7 1,567,702 - -1.5 1,567,881 - -0.1 1,567,882 - -1.1 1,567,939 - +1.3 1,568,056 + -0.5 1,568,064 - -1.2 1,568,064 - -0.7 1,568,064 - -0.3 1,568,064 - -0.3 1,568,426 + flhA Shewana3_1356 0.27 -1.6 1,568,505 + flhA Shewana3_1356 0.31 -1.0 1,568,549 + flhA Shewana3_1356 0.33 -0.9
Or see this region's nucleotide sequence