Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1117 and ispD are separated by 10 nucleotides ispD and ispF are separated by 51 nucleotides ispF and Shewana3_1120 are separated by 29 nucleotides
Shewana3_1117: Shewana3_1117 - cell division protein FtsB (RefSeq), at 1,317,147 to 1,317,446
_1117
Shewana3_1118: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (RefSeq), at 1,317,457 to 1,318,206
ispD
Shewana3_1119: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (RefSeq), at 1,318,258 to 1,318,737
ispF
Shewana3_1120: Shewana3_1120 - tRNA pseudouridine synthase D, TruD (RefSeq), at 1,318,767 to 1,319,894
_1120
Position (kb)
1318
1319 Strain fitness (log2 ratio)
-1
0
1
2 at 1318.727 kb on + strand at 1318.941 kb on - strand, within Shewana3_1120 at 1319.026 kb on - strand, within Shewana3_1120 at 1319.066 kb on + strand, within Shewana3_1120 at 1319.074 kb on - strand, within Shewana3_1120 at 1319.146 kb on + strand, within Shewana3_1120 at 1319.262 kb on + strand, within Shewana3_1120 at 1319.331 kb on + strand, within Shewana3_1120 at 1319.333 kb on + strand, within Shewana3_1120 at 1319.350 kb on + strand, within Shewana3_1120 at 1319.584 kb on + strand, within Shewana3_1120
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,318,727 + +0.1 1,318,941 - Shewana3_1120 0.15 +0.1 1,319,026 - Shewana3_1120 0.23 -0.5 1,319,066 + Shewana3_1120 0.27 +0.5 1,319,074 - Shewana3_1120 0.27 -1.6 1,319,146 + Shewana3_1120 0.34 -1.0 1,319,262 + Shewana3_1120 0.44 +0.6 1,319,331 + Shewana3_1120 0.50 +1.6 1,319,333 + Shewana3_1120 0.50 +1.1 1,319,350 + Shewana3_1120 0.52 +1.8 1,319,584 + Shewana3_1120 0.72 -0.0
Or see this region's nucleotide sequence