Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1032 and rpsO are separated by 126 nucleotides rpsO and Shewana3_1034 are separated by 86 nucleotides Shewana3_1034 and Shewana3_1035 are separated by 218 nucleotides
Shewana3_1032: Shewana3_1032 - tRNA pseudouridine synthase B (RefSeq), at 1,205,386 to 1,206,339
_1032
Shewana3_1033: rpsO - 30S ribosomal protein S15 (RefSeq), at 1,206,466 to 1,206,735
rpsO
Shewana3_1034: Shewana3_1034 - diguanylate cyclase/phosphodiesterase (RefSeq), at 1,206,822 to 1,208,846
_1034
Shewana3_1035: Shewana3_1035 - polynucleotide phosphorylase/polyadenylase (RefSeq), at 1,209,065 to 1,211,164
_1035
Position (kb)
1206
1207
1208
1209 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1206.307 kb on + strand at 1206.889 kb on + strand at 1207.138 kb on + strand, within Shewana3_1034 at 1207.192 kb on - strand, within Shewana3_1034 at 1207.804 kb on + strand, within Shewana3_1034 at 1207.867 kb on - strand, within Shewana3_1034 at 1208.336 kb on + strand, within Shewana3_1034 at 1208.336 kb on + strand, within Shewana3_1034 at 1208.344 kb on - strand, within Shewana3_1034 at 1208.354 kb on - strand, within Shewana3_1034 at 1208.839 kb on + strand at 1208.929 kb on + strand at 1208.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,206,307 + +1.6 1,206,889 + -0.4 1,207,138 + Shewana3_1034 0.16 -2.0 1,207,192 - Shewana3_1034 0.18 +0.2 1,207,804 + Shewana3_1034 0.48 -1.5 1,207,867 - Shewana3_1034 0.52 -1.1 1,208,336 + Shewana3_1034 0.75 -1.5 1,208,336 + Shewana3_1034 0.75 -2.6 1,208,344 - Shewana3_1034 0.75 -3.0 1,208,354 - Shewana3_1034 0.76 -0.5 1,208,839 + -0.2 1,208,929 + -0.4 1,208,937 - -1.4
Or see this region's nucleotide sequence