Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06499 and H281DRAFT_06500 are separated by 131 nucleotides H281DRAFT_06500 and H281DRAFT_06501 are separated by 510 nucleotides
H281DRAFT_06499: H281DRAFT_06499 - D-3-phosphoglycerate dehydrogenase, at 45,661 to 46,581
_06499
H281DRAFT_06500: H281DRAFT_06500 - Lactate dehydrogenase, at 46,713 to 47,639
_06500
H281DRAFT_06501: H281DRAFT_06501 - N-ethylmaleimide reductase, at 48,150 to 49,289
_06501
Position (kb)
46
47
48 Strain fitness (log2 ratio)
-2
-1
0
1 at 45.746 kb on - strand at 45.903 kb on + strand, within H281DRAFT_06499 at 46.148 kb on + strand, within H281DRAFT_06499 at 46.148 kb on + strand, within H281DRAFT_06499 at 46.149 kb on - strand, within H281DRAFT_06499 at 46.209 kb on + strand, within H281DRAFT_06499 at 46.209 kb on + strand, within H281DRAFT_06499 at 46.233 kb on + strand, within H281DRAFT_06499 at 46.461 kb on + strand, within H281DRAFT_06499 at 46.461 kb on + strand, within H281DRAFT_06499 at 46.580 kb on - strand at 46.673 kb on - strand at 46.712 kb on + strand at 46.712 kb on + strand at 46.712 kb on + strand at 46.864 kb on - strand, within H281DRAFT_06500 at 47.510 kb on - strand, within H281DRAFT_06500 at 47.565 kb on - strand at 47.660 kb on + strand at 47.720 kb on - strand at 47.924 kb on - strand at 47.924 kb on - strand at 48.039 kb on + strand at 48.040 kb on - strand at 48.079 kb on - strand at 48.138 kb on + strand at 48.189 kb on - strand at 48.279 kb on + strand, within H281DRAFT_06501 at 48.373 kb on - strand, within H281DRAFT_06501 at 48.440 kb on - strand, within H281DRAFT_06501
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x remove 45,746 - +0.0 45,903 + H281DRAFT_06499 0.26 +0.5 46,148 + H281DRAFT_06499 0.53 -2.2 46,148 + H281DRAFT_06499 0.53 +0.7 46,149 - H281DRAFT_06499 0.53 +0.1 46,209 + H281DRAFT_06499 0.60 -0.1 46,209 + H281DRAFT_06499 0.60 +1.4 46,233 + H281DRAFT_06499 0.62 -1.3 46,461 + H281DRAFT_06499 0.87 +1.2 46,461 + H281DRAFT_06499 0.87 -1.3 46,580 - +1.1 46,673 - -0.1 46,712 + -0.7 46,712 + +0.9 46,712 + -0.0 46,864 - H281DRAFT_06500 0.16 -2.0 47,510 - H281DRAFT_06500 0.86 -0.7 47,565 - +0.3 47,660 + -0.4 47,720 - +1.2 47,924 - +0.2 47,924 - +0.8 48,039 + +0.0 48,040 - -0.3 48,079 - -0.3 48,138 + -0.3 48,189 - +0.6 48,279 + H281DRAFT_06501 0.11 -1.2 48,373 - H281DRAFT_06501 0.20 -0.3 48,440 - H281DRAFT_06501 0.25 -1.9
Or see this region's nucleotide sequence