Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05087

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05086 and H281DRAFT_05087 overlap by 4 nucleotidesH281DRAFT_05087 and H281DRAFT_05088 are separated by 54 nucleotidesH281DRAFT_05088 and H281DRAFT_05089 are separated by 4 nucleotidesH281DRAFT_05089 and H281DRAFT_05090 overlap by 4 nucleotides H281DRAFT_05086: H281DRAFT_05086 - DNA helicase/exodeoxyribonuclease V, beta subunit, at 26,981 to 30,682 _05086 H281DRAFT_05087: H281DRAFT_05087 - exodeoxyribonuclease V alpha subunit, at 30,679 to 33,003 _05087 H281DRAFT_05088: H281DRAFT_05088 - hypothetical protein, at 33,058 to 33,201 _05088 H281DRAFT_05089: H281DRAFT_05089 - ribosome-associated protein, at 33,206 to 33,610 _05089 H281DRAFT_05090: H281DRAFT_05090 - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 33,607 to 34,932 _05090 Position (kb) 30 31 32 33 34Strain fitness (log2 ratio) -2 -1 0 1at 30.476 kb on + strandat 31.261 kb on - strand, within H281DRAFT_05087at 31.423 kb on - strand, within H281DRAFT_05087at 31.647 kb on + strand, within H281DRAFT_05087at 33.019 kb on - strandat 33.176 kb on - strand, within H281DRAFT_05088at 33.176 kb on - strand, within H281DRAFT_05088at 33.199 kb on + strandat 33.200 kb on - strandat 33.543 kb on + strand, within H281DRAFT_05089at 33.544 kb on - strand, within H281DRAFT_05089at 33.608 kb on + strandat 33.725 kb on + strandat 33.725 kb on + strandat 33.726 kb on - strandat 33.726 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
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30,476 + -0.7
31,261 - H281DRAFT_05087 0.25 -2.4
31,423 - H281DRAFT_05087 0.32 -2.2
31,647 + H281DRAFT_05087 0.42 -0.0
33,019 - -0.4
33,176 - H281DRAFT_05088 0.82 -0.1
33,176 - H281DRAFT_05088 0.82 +1.1
33,199 + +0.0
33,200 - +0.9
33,543 + H281DRAFT_05089 0.83 +0.4
33,544 - H281DRAFT_05089 0.83 -0.4
33,608 + -0.2
33,725 + -0.4
33,725 + -0.9
33,726 - -0.2
33,726 - -0.1

Or see this region's nucleotide sequence