Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02541

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02539 and H281DRAFT_02540 are separated by 39 nucleotidesH281DRAFT_02540 and H281DRAFT_02541 are separated by 3 nucleotidesH281DRAFT_02541 and H281DRAFT_02542 overlap by 1 nucleotides H281DRAFT_02539: H281DRAFT_02539 - Sugar phosphate permease, at 114,162 to 115,367 _02539 H281DRAFT_02540: H281DRAFT_02540 - 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33), at 115,407 to 116,009 _02540 H281DRAFT_02541: H281DRAFT_02541 - 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33), at 116,013 to 117,410 _02541 H281DRAFT_02542: H281DRAFT_02542 - 2-Methylisocitrate lyase, PEP mutase family, at 117,410 to 118,282 _02542 Position (kb) 116 117 118Strain fitness (log2 ratio) -2 -1 0 1at 115.312 kb on + strandat 115.312 kb on + strandat 115.408 kb on + strandat 116.909 kb on + strand, within H281DRAFT_02541at 116.955 kb on + strand, within H281DRAFT_02541at 116.955 kb on + strand, within H281DRAFT_02541at 116.956 kb on - strand, within H281DRAFT_02541at 117.494 kb on - strandat 117.640 kb on + strand, within H281DRAFT_02542at 117.641 kb on - strand, within H281DRAFT_02542at 118.205 kb on + strandat 118.205 kb on + strandat 118.312 kb on + strandat 118.312 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
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115,312 + +0.2
115,312 + +0.4
115,408 + -1.6
116,909 + H281DRAFT_02541 0.64 -0.8
116,955 + H281DRAFT_02541 0.67 -1.1
116,955 + H281DRAFT_02541 0.67 -0.6
116,956 - H281DRAFT_02541 0.67 -2.7
117,494 - -0.4
117,640 + H281DRAFT_02542 0.26 +0.3
117,641 - H281DRAFT_02542 0.26 -0.2
118,205 + -0.8
118,205 + -0.8
118,312 + -1.3
118,312 + -0.5

Or see this region's nucleotide sequence