Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02539 and H281DRAFT_02540 are separated by 39 nucleotides H281DRAFT_02540 and H281DRAFT_02541 are separated by 3 nucleotides H281DRAFT_02541 and H281DRAFT_02542 overlap by 1 nucleotides
H281DRAFT_02539: H281DRAFT_02539 - Sugar phosphate permease, at 114,162 to 115,367
_02539
H281DRAFT_02540: H281DRAFT_02540 - 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33), at 115,407 to 116,009
_02540
H281DRAFT_02541: H281DRAFT_02541 - 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33), at 116,013 to 117,410
_02541
H281DRAFT_02542: H281DRAFT_02542 - 2-Methylisocitrate lyase, PEP mutase family, at 117,410 to 118,282
_02542
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1 at 115.312 kb on + strand at 115.312 kb on + strand at 115.408 kb on + strand at 116.909 kb on + strand, within H281DRAFT_02541 at 116.955 kb on + strand, within H281DRAFT_02541 at 116.955 kb on + strand, within H281DRAFT_02541 at 116.956 kb on - strand, within H281DRAFT_02541 at 117.494 kb on - strand at 117.640 kb on + strand, within H281DRAFT_02542 at 117.641 kb on - strand, within H281DRAFT_02542 at 118.205 kb on + strand at 118.205 kb on + strand at 118.312 kb on + strand at 118.312 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x remove 115,312 + +0.2 115,312 + +0.4 115,408 + -1.6 116,909 + H281DRAFT_02541 0.64 -0.8 116,955 + H281DRAFT_02541 0.67 -1.1 116,955 + H281DRAFT_02541 0.67 -0.6 116,956 - H281DRAFT_02541 0.67 -2.7 117,494 - -0.4 117,640 + H281DRAFT_02542 0.26 +0.3 117,641 - H281DRAFT_02542 0.26 -0.2 118,205 + -0.8 118,205 + -0.8 118,312 + -1.3 118,312 + -0.5
Or see this region's nucleotide sequence