Experiment: MoYLS4 with Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2560 and DvMF_2561 are separated by 117 nucleotides DvMF_2561 and DvMF_2562 overlap by 4 nucleotides DvMF_2562 and DvMF_R0070 are separated by 123 nucleotides DvMF_R0070 and DvMF_2563 are separated by 203 nucleotides
DvMF_2560: DvMF_2560 - transcriptional regulator, AraC family (RefSeq), at 3,236,072 to 3,237,073
_2560
DvMF_2561: DvMF_2561 - hypothetical protein (RefSeq), at 3,237,191 to 3,237,337
_2561
DvMF_2562: DvMF_2562 - ThiJ/PfpI domain protein (RefSeq), at 3,237,334 to 3,237,930
_2562
DvMF_R0070: DvMF_R0070 - tRNA-Glu (RefSeq), at 3,238,054 to 3,238,130
_R0070
DvMF_2563: DvMF_2563 - protein of unknown function DUF721 (RefSeq), at 3,238,334 to 3,238,870
_2563
Position (kb) 3238
3239 Strain fitness (log2 ratio) -2
-1
0
1 at 3237.331 kb on + strand at 3237.590 kb on - strand, within DvMF_2562 at 3237.826 kb on - strand, within DvMF_2562 at 3238.020 kb on - strand at 3238.104 kb on + strand, within DvMF_R0070 at 3238.353 kb on + strand at 3238.392 kb on + strand, within DvMF_2563 at 3238.876 kb on + strand at 3239.023 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1 mg/ml remove 3,237,331 + -0.4 3,237,590 - DvMF_2562 0.43 -0.8 3,237,826 - DvMF_2562 0.82 -0.6 3,238,020 - -0.4 3,238,104 + DvMF_R0070 0.65 -1.8 3,238,353 + -1.7 3,238,392 + DvMF_2563 0.11 -1.0 3,238,876 + -1.0 3,239,023 - -0.7
Or see this region's nucleotide sequence