Experiment: MoYLS4 with Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1575 and DvMF_1576 are separated by 795 nucleotides DvMF_1576 and DvMF_1577 are separated by 170 nucleotides DvMF_1577 and DvMF_R0045 are separated by 200 nucleotides DvMF_R0045 and DvMF_R0046 are separated by 99 nucleotides DvMF_R0046 and DvMF_R0047 are separated by 6 nucleotides
DvMF_1575: DvMF_1575 - sulfate transporter (RefSeq), at 1,941,786 to 1,943,498
_1575
DvMF_1576: DvMF_1576 - transcriptional regulator, AraC family (RefSeq), at 1,944,294 to 1,945,604
_1576
DvMF_1577: DvMF_1577 - hypothetical protein (RefSeq), at 1,945,775 to 1,946,011
_1577
DvMF_R0045: DvMF_R0045 - tRNA-Asp (RefSeq), at 1,946,212 to 1,946,288
_R0045
DvMF_R0046: DvMF_R0046 - tRNA-Asp (RefSeq), at 1,946,388 to 1,946,464
_R0046
DvMF_R0047: DvMF_R0047 - tRNA-Val (RefSeq), at 1,946,471 to 1,946,546
_R0047
Position (kb)
1944
1945
1946 Strain fitness (log2 ratio)
-1
0
1 at 1943.374 kb on + strand at 1943.488 kb on + strand at 1943.496 kb on - strand at 1943.717 kb on - strand at 1943.719 kb on - strand at 1943.780 kb on + strand at 1943.942 kb on - strand at 1943.972 kb on + strand at 1944.261 kb on + strand at 1944.351 kb on + strand at 1944.361 kb on + strand at 1944.371 kb on + strand at 1944.549 kb on - strand, within DvMF_1576 at 1944.719 kb on - strand, within DvMF_1576 at 1944.806 kb on - strand, within DvMF_1576 at 1944.870 kb on - strand, within DvMF_1576 at 1944.939 kb on - strand, within DvMF_1576 at 1945.187 kb on + strand, within DvMF_1576 at 1945.638 kb on + strand at 1945.705 kb on - strand at 1945.768 kb on + strand at 1945.771 kb on - strand at 1945.894 kb on + strand, within DvMF_1577 at 1945.967 kb on + strand, within DvMF_1577 at 1946.070 kb on + strand at 1946.073 kb on - strand at 1946.075 kb on + strand at 1946.075 kb on + strand at 1946.083 kb on - strand at 1946.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1 mg/ml remove 1,943,374 + -0.7 1,943,488 + -0.8 1,943,496 - -0.9 1,943,717 - +0.1 1,943,719 - -1.0 1,943,780 + -0.3 1,943,942 - -0.7 1,943,972 + +0.2 1,944,261 + -0.9 1,944,351 + -0.6 1,944,361 + -1.0 1,944,371 + -0.6 1,944,549 - DvMF_1576 0.19 -0.8 1,944,719 - DvMF_1576 0.32 -0.6 1,944,806 - DvMF_1576 0.39 -0.2 1,944,870 - DvMF_1576 0.44 -1.0 1,944,939 - DvMF_1576 0.49 -0.3 1,945,187 + DvMF_1576 0.68 -0.2 1,945,638 + -0.5 1,945,705 - -0.1 1,945,768 + -0.2 1,945,771 - +0.6 1,945,894 + DvMF_1577 0.50 -0.1 1,945,967 + DvMF_1577 0.81 -0.6 1,946,070 + -0.3 1,946,073 - -0.2 1,946,075 + -0.5 1,946,075 + -0.7 1,946,083 - -1.5 1,946,083 - -0.2
Or see this region's nucleotide sequence