Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03375 and MMP_RS03380 are separated by 142 nucleotides MMP_RS03380 and MMP_RS03385 are separated by 4 nucleotides MMP_RS03385 and MMP_RS03390 are separated by 16 nucleotides
MMP_RS03375: MMP_RS03375 - methanogenesis marker 12 protein, at 633,847 to 634,824
_RS03375
MMP_RS03380: MMP_RS03380 - DUF354 domain-containing protein, at 634,967 to 635,980
_RS03380
MMP_RS03385: MMP_RS03385 - cation diffusion facilitator family transporter, at 635,985 to 636,860
_RS03385
MMP_RS03390: MMP_RS03390 - malate dehydrogenase, at 636,877 to 637,821
_RS03390
Position (kb) 634
635
636 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 634.127 kb on - strand at 634.333 kb on - strand at 634.397 kb on - strand at 634.398 kb on + strand at 634.588 kb on - strand at 634.655 kb on + strand at 634.843 kb on - strand at 634.847 kb on + strand at 634.867 kb on - strand at 634.870 kb on + strand at 634.874 kb on + strand at 634.884 kb on - strand at 634.884 kb on - strand at 634.974 kb on - strand at 635.869 kb on + strand at 635.973 kb on - strand at 635.978 kb on - strand at 636.014 kb on - strand at 636.290 kb on - strand, within MMP_RS03385 at 636.837 kb on + strand at 636.883 kb on + strand at 636.914 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 634,127 - -4.2 634,333 - -3.2 634,397 - -2.2 634,398 + -3.9 634,588 - -1.6 634,655 + -2.4 634,843 - +1.1 634,847 + +1.0 634,867 - -1.6 634,870 + -2.9 634,874 + -1.5 634,884 - -4.5 634,884 - -3.2 634,974 - -3.1 635,869 + -1.3 635,973 - -0.8 635,978 - +0.3 636,014 - -0.8 636,290 - MMP_RS03385 0.35 -0.7 636,837 + -0.3 636,883 + +0.1 636,914 + -4.2
Or see this region's nucleotide sequence