Experiment: PYE with Vancomycin 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01582 and CCNA_01583 are separated by 69 nucleotides CCNA_01583 and CCNA_03954 overlap by 239 nucleotides CCNA_03954 and CCNA_01584 are separated by 75 nucleotides
CCNA_01582: CCNA_01582 - Glutathione-dependent formaldehyde-activating enzyme, at 1,698,089 to 1,698,451
_01582
CCNA_01583: CCNA_01583 - phosphate-binding protein, at 1,698,521 to 1,699,561
_01583
CCNA_03954: CCNA_03954 - hypothetical protein, at 1,699,323 to 1,699,610
_03954
CCNA_01584: CCNA_01584 - multimodular transpeptidase-transglycosylase PBP 1A, at 1,699,686 to 1,702,070
_01584
Position (kb)
1699
1700 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1698.389 kb on - strand, within CCNA_01582 at 1698.396 kb on - strand, within CCNA_01582 at 1698.406 kb on + strand, within CCNA_01582 at 1698.421 kb on + strand at 1698.429 kb on - strand at 1698.721 kb on - strand, within CCNA_01583 at 1698.721 kb on - strand, within CCNA_01583 at 1698.762 kb on + strand, within CCNA_01583 at 1698.895 kb on - strand, within CCNA_01583 at 1699.027 kb on + strand, within CCNA_01583 at 1699.036 kb on + strand at 1699.044 kb on + strand, within CCNA_01583 at 1699.152 kb on - strand, within CCNA_01583 at 1699.164 kb on + strand, within CCNA_01583 at 1699.342 kb on - strand at 1699.484 kb on - strand at 1699.484 kb on - strand at 1699.578 kb on - strand, within CCNA_03954 at 1699.597 kb on - strand at 1699.607 kb on - strand at 1699.607 kb on - strand at 1699.678 kb on - strand at 1699.726 kb on - strand at 1699.786 kb on - strand at 1699.899 kb on - strand at 1699.939 kb on - strand, within CCNA_01584 at 1699.941 kb on + strand, within CCNA_01584 at 1699.971 kb on + strand, within CCNA_01584 at 1700.000 kb on - strand, within CCNA_01584 at 1700.013 kb on + strand, within CCNA_01584 at 1700.016 kb on - strand, within CCNA_01584 at 1700.021 kb on - strand, within CCNA_01584 at 1700.031 kb on - strand, within CCNA_01584 at 1700.063 kb on + strand, within CCNA_01584 at 1700.086 kb on + strand, within CCNA_01584 at 1700.124 kb on + strand, within CCNA_01584 at 1700.137 kb on + strand, within CCNA_01584 at 1700.139 kb on - strand, within CCNA_01584 at 1700.170 kb on - strand, within CCNA_01584 at 1700.202 kb on + strand, within CCNA_01584 at 1700.232 kb on + strand, within CCNA_01584 at 1700.240 kb on - strand, within CCNA_01584 at 1700.240 kb on - strand, within CCNA_01584 at 1700.244 kb on + strand, within CCNA_01584 at 1700.245 kb on - strand, within CCNA_01584 at 1700.250 kb on - strand, within CCNA_01584 at 1700.269 kb on - strand, within CCNA_01584 at 1700.276 kb on + strand, within CCNA_01584 at 1700.291 kb on + strand at 1700.299 kb on - strand, within CCNA_01584 at 1700.302 kb on + strand, within CCNA_01584 at 1700.320 kb on - strand, within CCNA_01584 at 1700.375 kb on - strand, within CCNA_01584 at 1700.390 kb on - strand, within CCNA_01584 at 1700.403 kb on + strand, within CCNA_01584 at 1700.411 kb on - strand, within CCNA_01584 at 1700.434 kb on + strand, within CCNA_01584 at 1700.439 kb on + strand, within CCNA_01584 at 1700.441 kb on + strand, within CCNA_01584 at 1700.452 kb on - strand, within CCNA_01584 at 1700.475 kb on + strand, within CCNA_01584 at 1700.483 kb on - strand, within CCNA_01584 at 1700.490 kb on - strand, within CCNA_01584 at 1700.553 kb on + strand, within CCNA_01584 at 1700.561 kb on + strand, within CCNA_01584 at 1700.565 kb on - strand, within CCNA_01584 at 1700.567 kb on + strand, within CCNA_01584 at 1700.569 kb on - strand, within CCNA_01584 at 1700.575 kb on - strand, within CCNA_01584 at 1700.592 kb on + strand, within CCNA_01584
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Vancomycin 0.007 mg/ml remove 1,698,389 - CCNA_01582 0.83 -0.8 1,698,396 - CCNA_01582 0.85 -0.7 1,698,406 + CCNA_01582 0.87 -0.2 1,698,421 + -0.7 1,698,429 - -0.6 1,698,721 - CCNA_01583 0.19 +1.8 1,698,721 - CCNA_01583 0.19 -0.9 1,698,762 + CCNA_01583 0.23 +0.5 1,698,895 - CCNA_01583 0.36 -0.1 1,699,027 + CCNA_01583 0.49 +1.1 1,699,036 + +2.3 1,699,044 + CCNA_01583 0.50 +0.9 1,699,152 - CCNA_01583 0.61 +1.5 1,699,164 + CCNA_01583 0.62 +0.6 1,699,342 - +0.3 1,699,484 - -1.6 1,699,484 - +1.9 1,699,578 - CCNA_03954 0.89 -1.8 1,699,597 - +0.6 1,699,607 - -0.2 1,699,607 - -0.5 1,699,678 - +1.4 1,699,726 - +0.1 1,699,786 - +0.1 1,699,899 - -0.3 1,699,939 - CCNA_01584 0.11 -1.8 1,699,941 + CCNA_01584 0.11 +1.1 1,699,971 + CCNA_01584 0.12 +1.7 1,700,000 - CCNA_01584 0.13 -1.1 1,700,013 + CCNA_01584 0.14 +0.7 1,700,016 - CCNA_01584 0.14 -0.9 1,700,021 - CCNA_01584 0.14 +0.6 1,700,031 - CCNA_01584 0.14 -1.2 1,700,063 + CCNA_01584 0.16 -0.5 1,700,086 + CCNA_01584 0.17 +0.6 1,700,124 + CCNA_01584 0.18 -1.3 1,700,137 + CCNA_01584 0.19 -1.8 1,700,139 - CCNA_01584 0.19 -1.2 1,700,170 - CCNA_01584 0.20 -1.8 1,700,202 + CCNA_01584 0.22 +0.4 1,700,232 + CCNA_01584 0.23 -1.3 1,700,240 - CCNA_01584 0.23 +0.2 1,700,240 - CCNA_01584 0.23 -1.6 1,700,244 + CCNA_01584 0.23 -2.4 1,700,245 - CCNA_01584 0.23 +1.0 1,700,250 - CCNA_01584 0.24 -1.2 1,700,269 - CCNA_01584 0.24 +0.8 1,700,276 + CCNA_01584 0.25 -0.0 1,700,291 + +2.5 1,700,299 - CCNA_01584 0.26 -1.3 1,700,302 + CCNA_01584 0.26 -1.9 1,700,320 - CCNA_01584 0.27 -1.0 1,700,375 - CCNA_01584 0.29 -0.9 1,700,390 - CCNA_01584 0.30 -0.3 1,700,403 + CCNA_01584 0.30 +2.7 1,700,411 - CCNA_01584 0.30 -0.4 1,700,434 + CCNA_01584 0.31 +0.5 1,700,439 + CCNA_01584 0.32 +0.6 1,700,441 + CCNA_01584 0.32 -0.4 1,700,452 - CCNA_01584 0.32 -1.6 1,700,475 + CCNA_01584 0.33 +0.8 1,700,483 - CCNA_01584 0.33 -0.0 1,700,490 - CCNA_01584 0.34 -0.5 1,700,553 + CCNA_01584 0.36 -1.4 1,700,561 + CCNA_01584 0.37 -0.3 1,700,565 - CCNA_01584 0.37 -0.6 1,700,567 + CCNA_01584 0.37 +0.3 1,700,569 - CCNA_01584 0.37 +2.3 1,700,575 - CCNA_01584 0.37 -2.8 1,700,592 + CCNA_01584 0.38 -0.4
Or see this region's nucleotide sequence