Experiment: PYE with Vancomycin 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01444 and CCNA_01445 are separated by 89 nucleotides CCNA_01445 and CCNA_01446 are separated by 108 nucleotides
CCNA_01444: CCNA_01444 - poly(3-hydroxyalkanoate) polymerase, at 1,555,589 to 1,557,607
_01444
CCNA_01445: CCNA_01445 - hypothetical protein, at 1,557,697 to 1,558,137
_01445
CCNA_01446: CCNA_01446 - LL-diaminopimelate aminotransferase, at 1,558,246 to 1,559,466
_01446
Position (kb)
1557
1558
1559 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1556.713 kb on + strand, within CCNA_01444 at 1556.741 kb on - strand, within CCNA_01444 at 1556.741 kb on - strand, within CCNA_01444 at 1556.744 kb on + strand, within CCNA_01444 at 1556.744 kb on + strand, within CCNA_01444 at 1556.855 kb on - strand, within CCNA_01444 at 1556.855 kb on - strand, within CCNA_01444 at 1556.916 kb on - strand, within CCNA_01444 at 1557.000 kb on + strand, within CCNA_01444 at 1557.008 kb on - strand, within CCNA_01444 at 1557.008 kb on - strand, within CCNA_01444 at 1557.008 kb on - strand, within CCNA_01444 at 1557.256 kb on - strand, within CCNA_01444 at 1557.349 kb on - strand, within CCNA_01444 at 1557.351 kb on + strand, within CCNA_01444 at 1557.359 kb on - strand, within CCNA_01444 at 1557.366 kb on + strand, within CCNA_01444 at 1557.374 kb on - strand, within CCNA_01444 at 1557.462 kb on + strand at 1557.470 kb on - strand at 1557.470 kb on - strand at 1557.471 kb on - strand at 1557.472 kb on + strand at 1557.490 kb on - strand at 1557.510 kb on + strand at 1557.510 kb on + strand at 1557.518 kb on - strand at 1557.518 kb on - strand at 1557.518 kb on - strand at 1557.567 kb on + strand at 1557.598 kb on - strand at 1557.608 kb on + strand at 1557.610 kb on - strand at 1557.611 kb on - strand at 1557.624 kb on + strand at 1557.656 kb on + strand at 1557.684 kb on - strand at 1557.738 kb on - strand at 1557.738 kb on - strand at 1557.796 kb on - strand, within CCNA_01445 at 1557.829 kb on + strand, within CCNA_01445 at 1557.840 kb on + strand, within CCNA_01445 at 1557.840 kb on + strand, within CCNA_01445 at 1557.840 kb on + strand, within CCNA_01445 at 1557.840 kb on + strand, within CCNA_01445 at 1557.840 kb on + strand, within CCNA_01445 at 1557.840 kb on + strand, within CCNA_01445 at 1557.840 kb on + strand, within CCNA_01445 at 1557.840 kb on + strand, within CCNA_01445 at 1557.848 kb on - strand, within CCNA_01445 at 1557.848 kb on - strand, within CCNA_01445 at 1557.848 kb on - strand, within CCNA_01445 at 1557.848 kb on - strand, within CCNA_01445 at 1557.887 kb on + strand, within CCNA_01445 at 1557.915 kb on + strand, within CCNA_01445 at 1557.923 kb on - strand, within CCNA_01445 at 1557.982 kb on + strand, within CCNA_01445 at 1557.984 kb on + strand, within CCNA_01445 at 1558.050 kb on - strand, within CCNA_01445 at 1558.075 kb on + strand, within CCNA_01445 at 1558.081 kb on + strand, within CCNA_01445 at 1558.096 kb on + strand at 1558.096 kb on + strand at 1558.114 kb on - strand at 1558.123 kb on + strand at 1558.173 kb on + strand at 1558.248 kb on - strand at 1558.290 kb on - strand at 1558.367 kb on - strand at 1558.381 kb on + strand, within CCNA_01446 at 1558.389 kb on - strand, within CCNA_01446 at 1558.423 kb on + strand, within CCNA_01446 at 1558.434 kb on + strand, within CCNA_01446 at 1558.457 kb on + strand, within CCNA_01446 at 1558.465 kb on - strand, within CCNA_01446 at 1558.473 kb on + strand, within CCNA_01446 at 1558.473 kb on + strand at 1558.474 kb on + strand, within CCNA_01446 at 1558.482 kb on - strand, within CCNA_01446 at 1558.482 kb on - strand, within CCNA_01446 at 1558.573 kb on + strand, within CCNA_01446 at 1558.576 kb on - strand, within CCNA_01446 at 1558.584 kb on + strand, within CCNA_01446 at 1558.587 kb on - strand, within CCNA_01446 at 1558.588 kb on + strand, within CCNA_01446 at 1558.618 kb on - strand, within CCNA_01446 at 1558.620 kb on + strand, within CCNA_01446 at 1558.623 kb on - strand, within CCNA_01446 at 1558.694 kb on + strand, within CCNA_01446 at 1558.704 kb on + strand, within CCNA_01446 at 1558.723 kb on + strand, within CCNA_01446 at 1558.731 kb on - strand, within CCNA_01446 at 1558.731 kb on - strand, within CCNA_01446 at 1558.761 kb on + strand, within CCNA_01446 at 1558.763 kb on - strand, within CCNA_01446 at 1558.790 kb on + strand, within CCNA_01446 at 1558.795 kb on + strand, within CCNA_01446 at 1558.849 kb on - strand, within CCNA_01446 at 1558.922 kb on + strand, within CCNA_01446 at 1558.956 kb on + strand, within CCNA_01446 at 1558.956 kb on + strand, within CCNA_01446 at 1558.956 kb on + strand, within CCNA_01446 at 1558.956 kb on + strand, within CCNA_01446 at 1558.956 kb on + strand, within CCNA_01446 at 1558.965 kb on - strand, within CCNA_01446 at 1559.013 kb on + strand, within CCNA_01446 at 1559.016 kb on - strand, within CCNA_01446 at 1559.021 kb on - strand, within CCNA_01446 at 1559.021 kb on - strand, within CCNA_01446 at 1559.040 kb on + strand, within CCNA_01446 at 1559.048 kb on - strand, within CCNA_01446 at 1559.061 kb on + strand, within CCNA_01446 at 1559.092 kb on + strand, within CCNA_01446 at 1559.092 kb on + strand, within CCNA_01446 at 1559.100 kb on - strand, within CCNA_01446 at 1559.100 kb on - strand, within CCNA_01446 at 1559.100 kb on - strand, within CCNA_01446 at 1559.102 kb on + strand, within CCNA_01446 at 1559.110 kb on - strand, within CCNA_01446 at 1559.112 kb on - strand, within CCNA_01446 at 1559.114 kb on + strand, within CCNA_01446
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Vancomycin 0.007 mg/ml remove 1,556,713 + CCNA_01444 0.56 +0.4 1,556,741 - CCNA_01444 0.57 +1.3 1,556,741 - CCNA_01444 0.57 +2.6 1,556,744 + CCNA_01444 0.57 +1.2 1,556,744 + CCNA_01444 0.57 -1.1 1,556,855 - CCNA_01444 0.63 +0.9 1,556,855 - CCNA_01444 0.63 +0.5 1,556,916 - CCNA_01444 0.66 +0.6 1,557,000 + CCNA_01444 0.70 +0.4 1,557,008 - CCNA_01444 0.70 +0.9 1,557,008 - CCNA_01444 0.70 -0.4 1,557,008 - CCNA_01444 0.70 +1.2 1,557,256 - CCNA_01444 0.83 -0.2 1,557,349 - CCNA_01444 0.87 +2.3 1,557,351 + CCNA_01444 0.87 +1.2 1,557,359 - CCNA_01444 0.88 +0.8 1,557,366 + CCNA_01444 0.88 +0.7 1,557,374 - CCNA_01444 0.88 +0.7 1,557,462 + -1.2 1,557,470 - +0.6 1,557,470 - +2.2 1,557,471 - -0.6 1,557,472 + -0.9 1,557,490 - -1.1 1,557,510 + -0.2 1,557,510 + +1.6 1,557,518 - -0.3 1,557,518 - +2.9 1,557,518 - -0.6 1,557,567 + +1.0 1,557,598 - +0.6 1,557,608 + -0.5 1,557,610 - +0.9 1,557,611 - -0.5 1,557,624 + -0.1 1,557,656 + -1.0 1,557,684 - -1.2 1,557,738 - +0.9 1,557,738 - -0.3 1,557,796 - CCNA_01445 0.22 -0.1 1,557,829 + CCNA_01445 0.30 -1.7 1,557,840 + CCNA_01445 0.32 +2.4 1,557,840 + CCNA_01445 0.32 -0.3 1,557,840 + CCNA_01445 0.32 -0.9 1,557,840 + CCNA_01445 0.32 -0.7 1,557,840 + CCNA_01445 0.32 -2.9 1,557,840 + CCNA_01445 0.32 -0.9 1,557,840 + CCNA_01445 0.32 +1.2 1,557,840 + CCNA_01445 0.32 -1.3 1,557,848 - CCNA_01445 0.34 -1.7 1,557,848 - CCNA_01445 0.34 +0.4 1,557,848 - CCNA_01445 0.34 +0.3 1,557,848 - CCNA_01445 0.34 +0.9 1,557,887 + CCNA_01445 0.43 -3.2 1,557,915 + CCNA_01445 0.49 -1.4 1,557,923 - CCNA_01445 0.51 +0.2 1,557,982 + CCNA_01445 0.65 -0.9 1,557,984 + CCNA_01445 0.65 +0.6 1,558,050 - CCNA_01445 0.80 -1.1 1,558,075 + CCNA_01445 0.86 -0.3 1,558,081 + CCNA_01445 0.87 -1.9 1,558,096 + -0.0 1,558,096 + -0.5 1,558,114 - +1.0 1,558,123 + +0.6 1,558,173 + -0.0 1,558,248 - -0.1 1,558,290 - -1.0 1,558,367 - -1.9 1,558,381 + CCNA_01446 0.11 +1.0 1,558,389 - CCNA_01446 0.12 +0.2 1,558,423 + CCNA_01446 0.14 +1.4 1,558,434 + CCNA_01446 0.15 +1.1 1,558,457 + CCNA_01446 0.17 -0.5 1,558,465 - CCNA_01446 0.18 +0.9 1,558,473 + CCNA_01446 0.19 +0.6 1,558,473 + +1.8 1,558,474 + CCNA_01446 0.19 +0.1 1,558,482 - CCNA_01446 0.19 +0.7 1,558,482 - CCNA_01446 0.19 +0.1 1,558,573 + CCNA_01446 0.27 +0.5 1,558,576 - CCNA_01446 0.27 -0.6 1,558,584 + CCNA_01446 0.28 +0.2 1,558,587 - CCNA_01446 0.28 -1.0 1,558,588 + CCNA_01446 0.28 -0.7 1,558,618 - CCNA_01446 0.30 -0.5 1,558,620 + CCNA_01446 0.31 -0.1 1,558,623 - CCNA_01446 0.31 -1.1 1,558,694 + CCNA_01446 0.37 -0.2 1,558,704 + CCNA_01446 0.38 +0.5 1,558,723 + CCNA_01446 0.39 +0.5 1,558,731 - CCNA_01446 0.40 -0.9 1,558,731 - CCNA_01446 0.40 -0.5 1,558,761 + CCNA_01446 0.42 +0.4 1,558,763 - CCNA_01446 0.42 -1.2 1,558,790 + CCNA_01446 0.45 -1.7 1,558,795 + CCNA_01446 0.45 +0.6 1,558,849 - CCNA_01446 0.49 -0.7 1,558,922 + CCNA_01446 0.55 +0.4 1,558,956 + CCNA_01446 0.58 +0.4 1,558,956 + CCNA_01446 0.58 -1.3 1,558,956 + CCNA_01446 0.58 -0.4 1,558,956 + CCNA_01446 0.58 +0.3 1,558,956 + CCNA_01446 0.58 -0.6 1,558,965 - CCNA_01446 0.59 +0.4 1,559,013 + CCNA_01446 0.63 +0.1 1,559,016 - CCNA_01446 0.63 -0.3 1,559,021 - CCNA_01446 0.63 +0.1 1,559,021 - CCNA_01446 0.63 +1.8 1,559,040 + CCNA_01446 0.65 +0.4 1,559,048 - CCNA_01446 0.66 +1.6 1,559,061 + CCNA_01446 0.67 +1.7 1,559,092 + CCNA_01446 0.69 +0.1 1,559,092 + CCNA_01446 0.69 +0.1 1,559,100 - CCNA_01446 0.70 -0.2 1,559,100 - CCNA_01446 0.70 +0.6 1,559,100 - CCNA_01446 0.70 +0.5 1,559,102 + CCNA_01446 0.70 +0.5 1,559,110 - CCNA_01446 0.71 +0.6 1,559,112 - CCNA_01446 0.71 +0.8 1,559,114 + CCNA_01446 0.71 +0.6
Or see this region's nucleotide sequence