Experiment: PYE with Vancomycin 0.007 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00250 and CCNA_00251 are separated by 48 nucleotides CCNA_00251 and CCNA_00252 are separated by 111 nucleotides CCNA_00252 and CCNA_00253 are separated by 253 nucleotides
CCNA_00250: CCNA_00250 - poly(3-hydroxyalkanoate) depolymerase, at 261,642 to 262,904
_00250
CCNA_00251: CCNA_00251 - zinc metalloprotease, at 262,953 to 263,672
_00251
CCNA_00252: CCNA_00252 - multimodular transpeptidase-transglycosylase PbpX, at 263,784 to 265,745
_00252
CCNA_00253: CCNA_00253 - aspartate-semialdehyde dehydrogenase, at 265,999 to 267,027
_00253
Position (kb) 263
264
265
266 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 262.791 kb on - strand at 262.814 kb on + strand at 262.849 kb on - strand at 262.906 kb on - strand at 262.923 kb on - strand at 262.950 kb on - strand at 262.950 kb on - strand at 262.968 kb on + strand at 262.968 kb on + strand at 263.016 kb on + strand at 263.021 kb on + strand at 263.029 kb on - strand, within CCNA_00251 at 263.069 kb on + strand, within CCNA_00251 at 263.074 kb on + strand, within CCNA_00251 at 263.095 kb on + strand, within CCNA_00251 at 263.101 kb on + strand, within CCNA_00251 at 263.101 kb on - strand, within CCNA_00251 at 263.146 kb on - strand, within CCNA_00251 at 263.204 kb on - strand, within CCNA_00251 at 263.216 kb on - strand, within CCNA_00251 at 263.294 kb on - strand, within CCNA_00251 at 263.316 kb on - strand, within CCNA_00251 at 263.333 kb on + strand, within CCNA_00251 at 263.340 kb on + strand, within CCNA_00251 at 263.340 kb on + strand, within CCNA_00251 at 263.348 kb on - strand, within CCNA_00251 at 263.373 kb on - strand, within CCNA_00251 at 263.382 kb on + strand, within CCNA_00251 at 263.382 kb on + strand, within CCNA_00251 at 263.382 kb on + strand, within CCNA_00251 at 263.383 kb on + strand, within CCNA_00251 at 263.390 kb on - strand, within CCNA_00251 at 263.446 kb on + strand, within CCNA_00251 at 263.455 kb on - strand, within CCNA_00251 at 263.530 kb on + strand, within CCNA_00251 at 263.547 kb on + strand, within CCNA_00251 at 263.552 kb on + strand, within CCNA_00251 at 263.555 kb on - strand, within CCNA_00251 at 263.576 kb on + strand, within CCNA_00251 at 263.578 kb on + strand, within CCNA_00251 at 263.665 kb on + strand at 263.698 kb on + strand at 263.711 kb on - strand at 263.718 kb on - strand at 263.720 kb on + strand at 263.728 kb on - strand at 263.728 kb on - strand at 263.732 kb on + strand at 263.736 kb on + strand at 263.739 kb on - strand at 263.759 kb on + strand at 263.768 kb on - strand at 263.785 kb on - strand at 263.790 kb on - strand at 263.806 kb on - strand at 263.834 kb on - strand at 263.889 kb on - strand at 263.904 kb on - strand at 263.935 kb on + strand at 263.967 kb on - strand at 263.971 kb on + strand at 263.971 kb on + strand at 263.971 kb on + strand at 263.971 kb on + strand at 264.025 kb on - strand, within CCNA_00252 at 264.027 kb on + strand, within CCNA_00252 at 264.030 kb on - strand, within CCNA_00252 at 264.035 kb on - strand, within CCNA_00252 at 264.035 kb on - strand, within CCNA_00252 at 264.036 kb on + strand, within CCNA_00252 at 264.036 kb on + strand, within CCNA_00252 at 264.085 kb on + strand, within CCNA_00252 at 264.093 kb on - strand, within CCNA_00252 at 264.093 kb on - strand, within CCNA_00252 at 264.200 kb on + strand, within CCNA_00252 at 264.213 kb on + strand, within CCNA_00252 at 264.241 kb on + strand, within CCNA_00252 at 264.253 kb on + strand, within CCNA_00252 at 264.261 kb on - strand, within CCNA_00252 at 264.261 kb on - strand, within CCNA_00252 at 264.265 kb on - strand, within CCNA_00252 at 264.290 kb on - strand, within CCNA_00252 at 264.300 kb on - strand, within CCNA_00252 at 264.300 kb on - strand, within CCNA_00252 at 264.329 kb on + strand, within CCNA_00252 at 264.329 kb on - strand, within CCNA_00252 at 264.331 kb on + strand, within CCNA_00252 at 264.336 kb on + strand, within CCNA_00252 at 264.372 kb on + strand, within CCNA_00252 at 264.397 kb on - strand, within CCNA_00252 at 264.421 kb on - strand, within CCNA_00252 at 264.436 kb on - strand, within CCNA_00252 at 264.495 kb on + strand, within CCNA_00252 at 264.495 kb on + strand, within CCNA_00252 at 264.512 kb on + strand, within CCNA_00252 at 264.520 kb on + strand, within CCNA_00252 at 264.520 kb on - strand, within CCNA_00252 at 264.582 kb on - strand, within CCNA_00252 at 264.800 kb on + strand, within CCNA_00252 at 264.800 kb on + strand, within CCNA_00252 at 264.844 kb on - strand, within CCNA_00252 at 264.891 kb on + strand, within CCNA_00252 at 264.891 kb on + strand, within CCNA_00252 at 264.913 kb on + strand, within CCNA_00252 at 264.936 kb on + strand, within CCNA_00252 at 264.946 kb on + strand, within CCNA_00252 at 264.954 kb on - strand, within CCNA_00252 at 264.994 kb on - strand, within CCNA_00252 at 264.994 kb on - strand, within CCNA_00252 at 265.025 kb on + strand, within CCNA_00252 at 265.036 kb on - strand, within CCNA_00252 at 265.203 kb on - strand, within CCNA_00252 at 265.235 kb on - strand, within CCNA_00252 at 265.250 kb on - strand, within CCNA_00252 at 265.250 kb on - strand, within CCNA_00252 at 265.293 kb on + strand, within CCNA_00252 at 265.293 kb on + strand, within CCNA_00252 at 265.293 kb on + strand, within CCNA_00252 at 265.324 kb on - strand, within CCNA_00252 at 265.339 kb on - strand, within CCNA_00252 at 265.339 kb on - strand, within CCNA_00252 at 265.340 kb on + strand, within CCNA_00252 at 265.361 kb on - strand, within CCNA_00252 at 265.385 kb on - strand, within CCNA_00252 at 265.418 kb on - strand, within CCNA_00252 at 265.535 kb on - strand, within CCNA_00252 at 265.552 kb on - strand at 265.570 kb on + strand at 265.576 kb on + strand at 265.578 kb on - strand at 265.579 kb on + strand at 265.586 kb on + strand at 265.594 kb on - strand at 265.619 kb on - strand at 265.636 kb on + strand at 265.680 kb on - strand at 265.684 kb on + strand at 265.746 kb on - strand at 265.755 kb on + strand at 265.771 kb on + strand at 265.771 kb on + strand at 265.771 kb on + strand at 265.786 kb on + strand at 265.792 kb on - strand at 265.794 kb on - strand at 265.800 kb on - strand at 265.802 kb on - strand at 265.803 kb on + strand at 265.852 kb on - strand at 265.858 kb on + strand at 265.890 kb on + strand at 265.892 kb on + strand at 265.904 kb on - strand at 265.911 kb on - strand at 265.941 kb on + strand at 265.982 kb on + strand at 265.982 kb on + strand at 265.982 kb on + strand at 265.982 kb on + strand at 265.984 kb on + strand at 265.984 kb on + strand at 265.984 kb on + strand at 265.984 kb on + strand at 265.984 kb on + strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.990 kb on - strand at 265.992 kb on - strand at 266.001 kb on + strand at 266.001 kb on + strand at 266.071 kb on + strand at 266.091 kb on + strand at 266.107 kb on - strand, within CCNA_00253 at 266.108 kb on + strand, within CCNA_00253 at 266.159 kb on + strand, within CCNA_00253 at 266.173 kb on - strand, within CCNA_00253 at 266.173 kb on - strand, within CCNA_00253 at 266.190 kb on + strand, within CCNA_00253 at 266.192 kb on - strand, within CCNA_00253 at 266.208 kb on - strand, within CCNA_00253 at 266.234 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.240 kb on - strand, within CCNA_00253 at 266.279 kb on + strand, within CCNA_00253 at 266.299 kb on + strand, within CCNA_00253 at 266.312 kb on + strand, within CCNA_00253 at 266.341 kb on + strand, within CCNA_00253 at 266.364 kb on + strand, within CCNA_00253 at 266.396 kb on + strand, within CCNA_00253 at 266.427 kb on - strand, within CCNA_00253 at 266.430 kb on + strand, within CCNA_00253 at 266.430 kb on + strand, within CCNA_00253 at 266.451 kb on + strand, within CCNA_00253 at 266.458 kb on + strand, within CCNA_00253 at 266.482 kb on + strand, within CCNA_00253 at 266.482 kb on + strand, within CCNA_00253 at 266.490 kb on + strand, within CCNA_00253 at 266.505 kb on - strand, within CCNA_00253 at 266.514 kb on + strand, within CCNA_00253 at 266.514 kb on + strand, within CCNA_00253 at 266.514 kb on + strand, within CCNA_00253 at 266.522 kb on - strand, within CCNA_00253 at 266.525 kb on + strand, within CCNA_00253 at 266.573 kb on + strand, within CCNA_00253 at 266.608 kb on + strand, within CCNA_00253 at 266.627 kb on - strand, within CCNA_00253 at 266.650 kb on + strand, within CCNA_00253 at 266.706 kb on + strand, within CCNA_00253 at 266.714 kb on - strand, within CCNA_00253 at 266.728 kb on - strand, within CCNA_00253 at 266.730 kb on + strand, within CCNA_00253
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Vancomycin 0.007 mg/ml remove 262,791 - -0.6 262,814 + -0.5 262,849 - +0.5 262,906 - +0.7 262,923 - -0.9 262,950 - +0.5 262,950 - +0.5 262,968 + +0.9 262,968 + -0.6 263,016 + -0.5 263,021 + -0.1 263,029 - CCNA_00251 0.11 -0.8 263,069 + CCNA_00251 0.16 +0.8 263,074 + CCNA_00251 0.17 -0.3 263,095 + CCNA_00251 0.20 +0.3 263,101 + CCNA_00251 0.21 -0.8 263,101 - CCNA_00251 0.21 -0.8 263,146 - CCNA_00251 0.27 -1.4 263,204 - CCNA_00251 0.35 -2.6 263,216 - CCNA_00251 0.37 -1.8 263,294 - CCNA_00251 0.47 -0.6 263,316 - CCNA_00251 0.50 -0.9 263,333 + CCNA_00251 0.53 +0.1 263,340 + CCNA_00251 0.54 +0.4 263,340 + CCNA_00251 0.54 -0.1 263,348 - CCNA_00251 0.55 -1.4 263,373 - CCNA_00251 0.58 -0.2 263,382 + CCNA_00251 0.60 -2.1 263,382 + CCNA_00251 0.60 -2.8 263,382 + CCNA_00251 0.60 -0.8 263,383 + CCNA_00251 0.60 +0.0 263,390 - CCNA_00251 0.61 +0.1 263,446 + CCNA_00251 0.68 -0.5 263,455 - CCNA_00251 0.70 -0.6 263,530 + CCNA_00251 0.80 +0.2 263,547 + CCNA_00251 0.82 +0.7 263,552 + CCNA_00251 0.83 -0.0 263,555 - CCNA_00251 0.84 -0.1 263,576 + CCNA_00251 0.87 -0.2 263,578 + CCNA_00251 0.87 -0.9 263,665 + +1.3 263,698 + +1.1 263,711 - -0.1 263,718 - +1.7 263,720 + -1.8 263,728 - +1.2 263,728 - +1.0 263,732 + -0.3 263,736 + -1.9 263,739 - -0.8 263,759 + -1.0 263,768 - -0.3 263,785 - +0.4 263,790 - +0.8 263,806 - -0.6 263,834 - -0.1 263,889 - +0.3 263,904 - -1.0 263,935 + -0.8 263,967 - -2.8 263,971 + -1.4 263,971 + -0.3 263,971 + +0.2 263,971 + -0.9 264,025 - CCNA_00252 0.12 -2.4 264,027 + CCNA_00252 0.12 -0.1 264,030 - CCNA_00252 0.13 +0.6 264,035 - CCNA_00252 0.13 -3.2 264,035 - CCNA_00252 0.13 -3.3 264,036 + CCNA_00252 0.13 +0.3 264,036 + CCNA_00252 0.13 +0.1 264,085 + CCNA_00252 0.15 -0.4 264,093 - CCNA_00252 0.16 -1.3 264,093 - CCNA_00252 0.16 -1.6 264,200 + CCNA_00252 0.21 -0.8 264,213 + CCNA_00252 0.22 -0.2 264,241 + CCNA_00252 0.23 -3.4 264,253 + CCNA_00252 0.24 -1.2 264,261 - CCNA_00252 0.24 -1.6 264,261 - CCNA_00252 0.24 +0.1 264,265 - CCNA_00252 0.25 -2.2 264,290 - CCNA_00252 0.26 +0.6 264,300 - CCNA_00252 0.26 -0.1 264,300 - CCNA_00252 0.26 -2.4 264,329 + CCNA_00252 0.28 +0.2 264,329 - CCNA_00252 0.28 -0.9 264,331 + CCNA_00252 0.28 -0.5 264,336 + CCNA_00252 0.28 -0.9 264,372 + CCNA_00252 0.30 +0.3 264,397 - CCNA_00252 0.31 -0.5 264,421 - CCNA_00252 0.32 -0.1 264,436 - CCNA_00252 0.33 -1.2 264,495 + CCNA_00252 0.36 -1.1 264,495 + CCNA_00252 0.36 -2.5 264,512 + CCNA_00252 0.37 -1.4 264,520 + CCNA_00252 0.38 +0.2 264,520 - CCNA_00252 0.38 -2.3 264,582 - CCNA_00252 0.41 -3.8 264,800 + CCNA_00252 0.52 +1.3 264,800 + CCNA_00252 0.52 -1.9 264,844 - CCNA_00252 0.54 -2.1 264,891 + CCNA_00252 0.56 +1.1 264,891 + CCNA_00252 0.56 -1.4 264,913 + CCNA_00252 0.58 -0.8 264,936 + CCNA_00252 0.59 -0.4 264,946 + CCNA_00252 0.59 -0.5 264,954 - CCNA_00252 0.60 +1.6 264,994 - CCNA_00252 0.62 -1.4 264,994 - CCNA_00252 0.62 +1.1 265,025 + CCNA_00252 0.63 +0.8 265,036 - CCNA_00252 0.64 +0.5 265,203 - CCNA_00252 0.72 -0.1 265,235 - CCNA_00252 0.74 -2.4 265,250 - CCNA_00252 0.75 -0.4 265,250 - CCNA_00252 0.75 -2.2 265,293 + CCNA_00252 0.77 +0.8 265,293 + CCNA_00252 0.77 -0.2 265,293 + CCNA_00252 0.77 +0.5 265,324 - CCNA_00252 0.78 -1.8 265,339 - CCNA_00252 0.79 -1.3 265,339 - CCNA_00252 0.79 -1.2 265,340 + CCNA_00252 0.79 -0.7 265,361 - CCNA_00252 0.80 -1.3 265,385 - CCNA_00252 0.82 -1.9 265,418 - CCNA_00252 0.83 -1.6 265,535 - CCNA_00252 0.89 -1.8 265,552 - +0.5 265,570 + -0.9 265,576 + -0.9 265,578 - +0.5 265,579 + -0.9 265,586 + -1.2 265,594 - +1.1 265,619 - -0.8 265,636 + +1.2 265,680 - -1.1 265,684 + -1.5 265,746 - -0.3 265,755 + -0.6 265,771 + -2.1 265,771 + -0.1 265,771 + -1.6 265,786 + -0.8 265,792 - -0.1 265,794 - -0.6 265,800 - -0.7 265,802 - -0.8 265,803 + -0.2 265,852 - -0.9 265,858 + -0.8 265,890 + -0.2 265,892 + -0.4 265,904 - -0.8 265,911 - -0.6 265,941 + -2.0 265,982 + -1.6 265,982 + +0.3 265,982 + -1.6 265,982 + +0.2 265,984 + -1.0 265,984 + +0.1 265,984 + -0.7 265,984 + -1.5 265,984 + -0.7 265,990 - +0.2 265,990 - +0.3 265,990 - -0.8 265,990 - +0.3 265,990 - +1.1 265,990 - +0.1 265,990 - +0.8 265,992 - -1.7 266,001 + -1.1 266,001 + -1.4 266,071 + -0.8 266,091 + +1.2 266,107 - CCNA_00253 0.10 +0.6 266,108 + CCNA_00253 0.11 -0.3 266,159 + CCNA_00253 0.16 -0.9 266,173 - CCNA_00253 0.17 +1.3 266,173 - CCNA_00253 0.17 -0.9 266,190 + CCNA_00253 0.19 -0.7 266,192 - CCNA_00253 0.19 -1.1 266,208 - CCNA_00253 0.20 -1.0 266,234 - CCNA_00253 0.23 -1.7 266,240 - CCNA_00253 0.23 -0.5 266,240 - CCNA_00253 0.23 -1.6 266,240 - CCNA_00253 0.23 +0.2 266,240 - CCNA_00253 0.23 +0.6 266,240 - CCNA_00253 0.23 -1.4 266,240 - CCNA_00253 0.23 -0.4 266,279 + CCNA_00253 0.27 +3.6 266,299 + CCNA_00253 0.29 -0.7 266,312 + CCNA_00253 0.30 -0.4 266,341 + CCNA_00253 0.33 +0.9 266,364 + CCNA_00253 0.35 +1.8 266,396 + CCNA_00253 0.39 -0.9 266,427 - CCNA_00253 0.42 -0.7 266,430 + CCNA_00253 0.42 +1.4 266,430 + CCNA_00253 0.42 -0.4 266,451 + CCNA_00253 0.44 -0.5 266,458 + CCNA_00253 0.45 -0.5 266,482 + CCNA_00253 0.47 -0.0 266,482 + CCNA_00253 0.47 -0.8 266,490 + CCNA_00253 0.48 +0.6 266,505 - CCNA_00253 0.49 +1.1 266,514 + CCNA_00253 0.50 +1.3 266,514 + CCNA_00253 0.50 +1.3 266,514 + CCNA_00253 0.50 -0.5 266,522 - CCNA_00253 0.51 -0.4 266,525 + CCNA_00253 0.51 -1.5 266,573 + CCNA_00253 0.56 +0.1 266,608 + CCNA_00253 0.59 -1.2 266,627 - CCNA_00253 0.61 -0.4 266,650 + CCNA_00253 0.63 -0.3 266,706 + CCNA_00253 0.69 -0.3 266,714 - CCNA_00253 0.69 -1.4 266,728 - CCNA_00253 0.71 -0.3 266,730 + CCNA_00253 0.71 +0.2
Or see this region's nucleotide sequence