Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16245 and BPHYT_RS16250 are separated by 118 nucleotides BPHYT_RS16250 and BPHYT_RS16255 are separated by 231 nucleotides BPHYT_RS16255 and BPHYT_RS16260 are separated by 132 nucleotides
BPHYT_RS16245: BPHYT_RS16245 - phosphoribosylaminoimidazole carboxylase, at 3,688,057 to 3,689,253
_RS16245
BPHYT_RS16250: BPHYT_RS16250 - N5-carboxyaminoimidazole ribonucleotide mutase, at 3,689,372 to 3,689,890
_RS16250
BPHYT_RS16255: BPHYT_RS16255 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 3,690,122 to 3,691,012
_RS16255
BPHYT_RS16260: BPHYT_RS16260 - fructose-bisphosphate aldolase, at 3,691,145 to 3,692,209
_RS16260
Position (kb)
3690
3691
3692 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3689.257 kb on - strand at 3689.257 kb on - strand at 3689.257 kb on - strand at 3689.301 kb on + strand at 3689.301 kb on + strand at 3689.301 kb on + strand at 3689.302 kb on - strand at 3689.302 kb on - strand at 3689.374 kb on - strand at 3689.587 kb on - strand, within BPHYT_RS16250 at 3689.894 kb on - strand at 3689.894 kb on - strand at 3690.016 kb on - strand at 3690.166 kb on - strand at 3690.427 kb on - strand, within BPHYT_RS16255 at 3690.445 kb on - strand, within BPHYT_RS16255 at 3690.445 kb on - strand, within BPHYT_RS16255 at 3690.445 kb on - strand, within BPHYT_RS16255 at 3690.445 kb on - strand, within BPHYT_RS16255 at 3690.446 kb on + strand, within BPHYT_RS16255 at 3690.447 kb on - strand, within BPHYT_RS16255 at 3690.447 kb on - strand, within BPHYT_RS16255 at 3690.943 kb on - strand at 3690.943 kb on - strand at 3690.943 kb on - strand at 3691.007 kb on - strand at 3691.099 kb on + strand at 3691.100 kb on - strand at 3691.179 kb on + strand at 3691.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 3,689,257 - +1.4 3,689,257 - -1.3 3,689,257 - +0.1 3,689,301 + -1.6 3,689,301 + +1.5 3,689,301 + +1.0 3,689,302 - +0.2 3,689,302 - -2.6 3,689,374 - -1.1 3,689,587 - BPHYT_RS16250 0.41 -3.4 3,689,894 - -0.1 3,689,894 - -0.2 3,690,016 - -0.9 3,690,166 - +1.1 3,690,427 - BPHYT_RS16255 0.34 -2.9 3,690,445 - BPHYT_RS16255 0.36 -0.0 3,690,445 - BPHYT_RS16255 0.36 -1.9 3,690,445 - BPHYT_RS16255 0.36 -6.0 3,690,445 - BPHYT_RS16255 0.36 -4.1 3,690,446 + BPHYT_RS16255 0.36 -0.8 3,690,447 - BPHYT_RS16255 0.36 -0.8 3,690,447 - BPHYT_RS16255 0.36 -2.9 3,690,943 - -2.8 3,690,943 - -3.0 3,690,943 - -2.1 3,691,007 - -2.4 3,691,099 + -3.9 3,691,100 - -3.1 3,691,179 + -1.9 3,691,180 - +0.7
Or see this region's nucleotide sequence