Experiment: Recovery broth with Penicillin G 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16830 and BBR_RS16835 are separated by 151 nucleotides BBR_RS16835 and BBR_RS16840 are separated by 33 nucleotides BBR_RS16840 and BBR_RS16845 are separated by 44 nucleotides
BBR_RS16830: BBR_RS16830 - peptidoglycan bridge formation glycyltransferase FemA/FemB family protein, at 1,544,722 to 1,545,729
_RS16830
BBR_RS16835: BBR_RS16835 - cell wall biosynthesis-associated protein, at 1,545,881 to 1,546,336
_RS16835
BBR_RS16840: BBR_RS16840 - chromosome segregation protein SMC, at 1,546,370 to 1,550,017
_RS16840
BBR_RS16845: BBR_RS16845 - bifunctional folylpolyglutamate synthase/dihydrofolate synthase, at 1,550,062 to 1,551,663
_RS16845
Position (kb)
1546
1547
1548
1549
1550
1551 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1545.707 kb on + strand at 1545.830 kb on - strand at 1545.830 kb on - strand at 1545.878 kb on - strand at 1545.879 kb on - strand at 1545.932 kb on + strand, within BBR_RS16835 at 1545.940 kb on - strand, within BBR_RS16835 at 1545.949 kb on + strand, within BBR_RS16835 at 1545.987 kb on + strand, within BBR_RS16835 at 1545.992 kb on + strand, within BBR_RS16835 at 1546.041 kb on + strand, within BBR_RS16835 at 1546.070 kb on - strand, within BBR_RS16835 at 1546.103 kb on + strand, within BBR_RS16835 at 1546.132 kb on + strand, within BBR_RS16835 at 1546.167 kb on + strand, within BBR_RS16835 at 1546.204 kb on - strand, within BBR_RS16835 at 1546.216 kb on + strand, within BBR_RS16835 at 1546.223 kb on + strand, within BBR_RS16835 at 1546.301 kb on + strand at 1546.311 kb on + strand at 1546.319 kb on - strand at 1546.460 kb on + strand at 1546.484 kb on - strand at 1546.520 kb on + strand at 1546.551 kb on + strand at 1546.561 kb on + strand at 1546.634 kb on - strand at 1546.649 kb on - strand at 1546.699 kb on + strand at 1546.699 kb on + strand at 1546.707 kb on - strand at 1546.709 kb on - strand at 1546.725 kb on - strand at 1546.749 kb on - strand, within BBR_RS16840 at 1546.760 kb on - strand, within BBR_RS16840 at 1546.809 kb on + strand, within BBR_RS16840 at 1546.817 kb on - strand, within BBR_RS16840 at 1546.826 kb on - strand, within BBR_RS16840 at 1546.892 kb on - strand, within BBR_RS16840 at 1546.934 kb on - strand, within BBR_RS16840 at 1546.950 kb on + strand, within BBR_RS16840 at 1546.994 kb on - strand, within BBR_RS16840 at 1547.031 kb on + strand, within BBR_RS16840 at 1547.048 kb on - strand, within BBR_RS16840 at 1547.072 kb on + strand, within BBR_RS16840 at 1547.077 kb on + strand, within BBR_RS16840 at 1547.120 kb on + strand, within BBR_RS16840 at 1547.126 kb on + strand, within BBR_RS16840 at 1547.150 kb on - strand, within BBR_RS16840 at 1547.217 kb on - strand, within BBR_RS16840 at 1547.217 kb on - strand, within BBR_RS16840 at 1547.251 kb on - strand, within BBR_RS16840 at 1547.281 kb on + strand, within BBR_RS16840 at 1547.360 kb on - strand, within BBR_RS16840 at 1547.384 kb on + strand, within BBR_RS16840 at 1547.394 kb on - strand, within BBR_RS16840 at 1547.414 kb on + strand, within BBR_RS16840 at 1547.538 kb on - strand, within BBR_RS16840 at 1547.579 kb on - strand, within BBR_RS16840 at 1547.590 kb on + strand, within BBR_RS16840 at 1547.637 kb on - strand, within BBR_RS16840 at 1547.655 kb on - strand, within BBR_RS16840 at 1547.655 kb on - strand, within BBR_RS16840 at 1547.669 kb on + strand, within BBR_RS16840 at 1547.680 kb on + strand, within BBR_RS16840 at 1547.710 kb on - strand, within BBR_RS16840 at 1547.776 kb on + strand, within BBR_RS16840 at 1547.833 kb on + strand, within BBR_RS16840 at 1547.833 kb on + strand, within BBR_RS16840 at 1547.838 kb on + strand, within BBR_RS16840 at 1547.841 kb on - strand, within BBR_RS16840 at 1547.841 kb on - strand, within BBR_RS16840 at 1547.841 kb on - strand, within BBR_RS16840 at 1547.846 kb on - strand, within BBR_RS16840 at 1547.903 kb on - strand, within BBR_RS16840 at 1547.999 kb on + strand, within BBR_RS16840 at 1548.064 kb on + strand, within BBR_RS16840 at 1548.081 kb on + strand, within BBR_RS16840 at 1548.098 kb on + strand, within BBR_RS16840 at 1548.117 kb on - strand, within BBR_RS16840 at 1548.130 kb on - strand, within BBR_RS16840 at 1548.173 kb on - strand, within BBR_RS16840 at 1548.187 kb on - strand, within BBR_RS16840 at 1548.199 kb on + strand, within BBR_RS16840 at 1548.210 kb on - strand, within BBR_RS16840 at 1548.214 kb on + strand, within BBR_RS16840 at 1548.218 kb on + strand, within BBR_RS16840 at 1548.227 kb on - strand, within BBR_RS16840 at 1548.257 kb on - strand, within BBR_RS16840 at 1548.283 kb on - strand, within BBR_RS16840 at 1548.361 kb on - strand, within BBR_RS16840 at 1548.542 kb on + strand, within BBR_RS16840 at 1548.551 kb on + strand, within BBR_RS16840 at 1548.557 kb on - strand, within BBR_RS16840 at 1548.559 kb on + strand, within BBR_RS16840 at 1548.559 kb on - strand, within BBR_RS16840 at 1548.567 kb on + strand, within BBR_RS16840 at 1548.567 kb on + strand, within BBR_RS16840 at 1548.575 kb on - strand, within BBR_RS16840 at 1548.596 kb on - strand, within BBR_RS16840 at 1548.596 kb on - strand, within BBR_RS16840 at 1548.596 kb on - strand, within BBR_RS16840 at 1548.596 kb on - strand, within BBR_RS16840 at 1548.604 kb on - strand, within BBR_RS16840 at 1548.626 kb on + strand, within BBR_RS16840 at 1548.642 kb on - strand, within BBR_RS16840 at 1548.642 kb on - strand, within BBR_RS16840 at 1548.642 kb on - strand, within BBR_RS16840 at 1548.642 kb on - strand, within BBR_RS16840 at 1548.654 kb on - strand, within BBR_RS16840 at 1548.663 kb on + strand, within BBR_RS16840 at 1548.777 kb on + strand, within BBR_RS16840 at 1548.876 kb on + strand, within BBR_RS16840 at 1548.884 kb on - strand, within BBR_RS16840 at 1548.896 kb on + strand, within BBR_RS16840 at 1548.896 kb on + strand, within BBR_RS16840 at 1548.896 kb on + strand, within BBR_RS16840 at 1548.958 kb on - strand, within BBR_RS16840 at 1548.989 kb on - strand, within BBR_RS16840 at 1548.989 kb on - strand, within BBR_RS16840 at 1548.989 kb on - strand, within BBR_RS16840 at 1549.007 kb on + strand, within BBR_RS16840 at 1549.023 kb on + strand, within BBR_RS16840 at 1549.105 kb on + strand, within BBR_RS16840 at 1549.113 kb on - strand, within BBR_RS16840 at 1549.218 kb on + strand, within BBR_RS16840 at 1549.220 kb on - strand, within BBR_RS16840 at 1549.225 kb on - strand, within BBR_RS16840 at 1549.226 kb on - strand, within BBR_RS16840 at 1549.226 kb on - strand, within BBR_RS16840 at 1549.226 kb on - strand, within BBR_RS16840 at 1549.226 kb on - strand, within BBR_RS16840 at 1549.228 kb on + strand, within BBR_RS16840 at 1549.228 kb on + strand, within BBR_RS16840 at 1549.251 kb on - strand, within BBR_RS16840 at 1549.253 kb on + strand, within BBR_RS16840 at 1549.321 kb on + strand, within BBR_RS16840 at 1549.347 kb on - strand, within BBR_RS16840 at 1549.361 kb on - strand, within BBR_RS16840 at 1549.475 kb on - strand, within BBR_RS16840 at 1549.484 kb on - strand, within BBR_RS16840 at 1549.508 kb on - strand, within BBR_RS16840 at 1549.508 kb on - strand, within BBR_RS16840 at 1549.510 kb on + strand, within BBR_RS16840 at 1549.558 kb on + strand, within BBR_RS16840 at 1549.790 kb on + strand at 1549.790 kb on + strand at 1549.792 kb on + strand at 1549.798 kb on - strand at 1549.798 kb on - strand at 1549.800 kb on - strand at 1549.842 kb on - strand at 1549.842 kb on - strand at 1549.853 kb on - strand at 1549.865 kb on + strand at 1549.865 kb on + strand at 1549.878 kb on + strand at 1549.886 kb on - strand at 1549.886 kb on - strand at 1549.944 kb on - strand at 1550.026 kb on - strand at 1550.042 kb on + strand at 1550.042 kb on + strand at 1550.050 kb on - strand at 1550.063 kb on - strand at 1550.081 kb on + strand at 1550.094 kb on - strand at 1550.094 kb on - strand at 1550.124 kb on + strand at 1550.130 kb on + strand at 1550.168 kb on + strand at 1550.176 kb on - strand at 1550.195 kb on + strand at 1550.213 kb on - strand at 1550.258 kb on + strand, within BBR_RS16845 at 1550.258 kb on + strand, within BBR_RS16845 at 1550.274 kb on + strand, within BBR_RS16845 at 1550.279 kb on + strand, within BBR_RS16845
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Penicillin G 2e-04 mM remove 1,545,707 + +0.2 1,545,830 - -0.1 1,545,830 - -0.0 1,545,878 - +0.5 1,545,879 - +2.0 1,545,932 + BBR_RS16835 0.11 +0.7 1,545,940 - BBR_RS16835 0.13 +0.2 1,545,949 + BBR_RS16835 0.15 -1.9 1,545,987 + BBR_RS16835 0.23 +0.3 1,545,992 + BBR_RS16835 0.24 +0.5 1,546,041 + BBR_RS16835 0.35 +1.0 1,546,070 - BBR_RS16835 0.41 -0.1 1,546,103 + BBR_RS16835 0.49 +0.9 1,546,132 + BBR_RS16835 0.55 -2.0 1,546,167 + BBR_RS16835 0.63 +1.6 1,546,204 - BBR_RS16835 0.71 +2.0 1,546,216 + BBR_RS16835 0.73 +0.5 1,546,223 + BBR_RS16835 0.75 +0.2 1,546,301 + -0.3 1,546,311 + +1.9 1,546,319 - -0.5 1,546,460 + -1.3 1,546,484 - -0.7 1,546,520 + +0.2 1,546,551 + +2.7 1,546,561 + +0.8 1,546,634 - -0.5 1,546,649 - +0.9 1,546,699 + -1.8 1,546,699 + -0.5 1,546,707 - +0.3 1,546,709 - +0.2 1,546,725 - +1.2 1,546,749 - BBR_RS16840 0.10 -0.6 1,546,760 - BBR_RS16840 0.11 +0.9 1,546,809 + BBR_RS16840 0.12 -0.5 1,546,817 - BBR_RS16840 0.12 -0.3 1,546,826 - BBR_RS16840 0.12 -0.3 1,546,892 - BBR_RS16840 0.14 -0.8 1,546,934 - BBR_RS16840 0.15 +0.8 1,546,950 + BBR_RS16840 0.16 -1.2 1,546,994 - BBR_RS16840 0.17 -0.5 1,547,031 + BBR_RS16840 0.18 -1.1 1,547,048 - BBR_RS16840 0.19 +0.3 1,547,072 + BBR_RS16840 0.19 -1.2 1,547,077 + BBR_RS16840 0.19 +0.0 1,547,120 + BBR_RS16840 0.21 +1.1 1,547,126 + BBR_RS16840 0.21 +2.0 1,547,150 - BBR_RS16840 0.21 -0.3 1,547,217 - BBR_RS16840 0.23 +0.2 1,547,217 - BBR_RS16840 0.23 +0.7 1,547,251 - BBR_RS16840 0.24 -0.7 1,547,281 + BBR_RS16840 0.25 -0.8 1,547,360 - BBR_RS16840 0.27 +1.0 1,547,384 + BBR_RS16840 0.28 -0.7 1,547,394 - BBR_RS16840 0.28 +0.0 1,547,414 + BBR_RS16840 0.29 +1.0 1,547,538 - BBR_RS16840 0.32 +0.8 1,547,579 - BBR_RS16840 0.33 -1.2 1,547,590 + BBR_RS16840 0.33 -1.5 1,547,637 - BBR_RS16840 0.35 -0.1 1,547,655 - BBR_RS16840 0.35 -1.9 1,547,655 - BBR_RS16840 0.35 -1.4 1,547,669 + BBR_RS16840 0.36 +1.6 1,547,680 + BBR_RS16840 0.36 -1.2 1,547,710 - BBR_RS16840 0.37 -1.1 1,547,776 + BBR_RS16840 0.39 -1.2 1,547,833 + BBR_RS16840 0.40 -0.3 1,547,833 + BBR_RS16840 0.40 -0.6 1,547,838 + BBR_RS16840 0.40 -0.0 1,547,841 - BBR_RS16840 0.40 +0.1 1,547,841 - BBR_RS16840 0.40 +0.3 1,547,841 - BBR_RS16840 0.40 +0.2 1,547,846 - BBR_RS16840 0.40 -0.7 1,547,903 - BBR_RS16840 0.42 -2.7 1,547,999 + BBR_RS16840 0.45 -1.3 1,548,064 + BBR_RS16840 0.46 -1.1 1,548,081 + BBR_RS16840 0.47 -1.9 1,548,098 + BBR_RS16840 0.47 +0.2 1,548,117 - BBR_RS16840 0.48 -0.3 1,548,130 - BBR_RS16840 0.48 -1.3 1,548,173 - BBR_RS16840 0.49 -1.0 1,548,187 - BBR_RS16840 0.50 -0.6 1,548,199 + BBR_RS16840 0.50 -2.4 1,548,210 - BBR_RS16840 0.50 -0.6 1,548,214 + BBR_RS16840 0.51 +1.8 1,548,218 + BBR_RS16840 0.51 -0.8 1,548,227 - BBR_RS16840 0.51 +0.8 1,548,257 - BBR_RS16840 0.52 -0.7 1,548,283 - BBR_RS16840 0.52 -0.8 1,548,361 - BBR_RS16840 0.55 +0.0 1,548,542 + BBR_RS16840 0.60 +0.3 1,548,551 + BBR_RS16840 0.60 +1.6 1,548,557 - BBR_RS16840 0.60 -0.5 1,548,559 + BBR_RS16840 0.60 -2.0 1,548,559 - BBR_RS16840 0.60 -1.5 1,548,567 + BBR_RS16840 0.60 +0.6 1,548,567 + BBR_RS16840 0.60 -0.3 1,548,575 - BBR_RS16840 0.60 +0.0 1,548,596 - BBR_RS16840 0.61 -0.7 1,548,596 - BBR_RS16840 0.61 -0.2 1,548,596 - BBR_RS16840 0.61 -0.6 1,548,596 - BBR_RS16840 0.61 -1.5 1,548,604 - BBR_RS16840 0.61 +0.2 1,548,626 + BBR_RS16840 0.62 -1.0 1,548,642 - BBR_RS16840 0.62 +0.3 1,548,642 - BBR_RS16840 0.62 +0.3 1,548,642 - BBR_RS16840 0.62 -1.2 1,548,642 - BBR_RS16840 0.62 -0.5 1,548,654 - BBR_RS16840 0.63 +1.3 1,548,663 + BBR_RS16840 0.63 -0.5 1,548,777 + BBR_RS16840 0.66 +0.2 1,548,876 + BBR_RS16840 0.69 +0.3 1,548,884 - BBR_RS16840 0.69 -0.8 1,548,896 + BBR_RS16840 0.69 -1.4 1,548,896 + BBR_RS16840 0.69 +0.8 1,548,896 + BBR_RS16840 0.69 -1.4 1,548,958 - BBR_RS16840 0.71 -0.1 1,548,989 - BBR_RS16840 0.72 -1.5 1,548,989 - BBR_RS16840 0.72 -0.5 1,548,989 - BBR_RS16840 0.72 +1.2 1,549,007 + BBR_RS16840 0.72 +0.0 1,549,023 + BBR_RS16840 0.73 -0.8 1,549,105 + BBR_RS16840 0.75 +0.6 1,549,113 - BBR_RS16840 0.75 +0.9 1,549,218 + BBR_RS16840 0.78 -0.0 1,549,220 - BBR_RS16840 0.78 +0.0 1,549,225 - BBR_RS16840 0.78 +0.5 1,549,226 - BBR_RS16840 0.78 +0.0 1,549,226 - BBR_RS16840 0.78 +0.1 1,549,226 - BBR_RS16840 0.78 -1.1 1,549,226 - BBR_RS16840 0.78 +0.3 1,549,228 + BBR_RS16840 0.78 -2.3 1,549,228 + BBR_RS16840 0.78 -1.7 1,549,251 - BBR_RS16840 0.79 +0.0 1,549,253 + BBR_RS16840 0.79 +0.9 1,549,321 + BBR_RS16840 0.81 -2.1 1,549,347 - BBR_RS16840 0.82 -1.5 1,549,361 - BBR_RS16840 0.82 -0.5 1,549,475 - BBR_RS16840 0.85 -2.4 1,549,484 - BBR_RS16840 0.85 -1.4 1,549,508 - BBR_RS16840 0.86 +1.3 1,549,508 - BBR_RS16840 0.86 +0.6 1,549,510 + BBR_RS16840 0.86 +0.2 1,549,558 + BBR_RS16840 0.87 -0.7 1,549,790 + +0.0 1,549,790 + -0.5 1,549,792 + -1.3 1,549,798 - +0.5 1,549,798 - +0.0 1,549,800 - +0.6 1,549,842 - -0.5 1,549,842 - -0.2 1,549,853 - -2.2 1,549,865 + +1.0 1,549,865 + -1.0 1,549,878 + +0.5 1,549,886 - +0.3 1,549,886 - -1.0 1,549,944 - +0.8 1,550,026 - -0.1 1,550,042 + -2.2 1,550,042 + -0.3 1,550,050 - -0.6 1,550,063 - +0.2 1,550,081 + +0.9 1,550,094 - +1.0 1,550,094 - +0.4 1,550,124 + -0.5 1,550,130 + +0.7 1,550,168 + +0.7 1,550,176 - +0.2 1,550,195 + -0.7 1,550,213 - +0.2 1,550,258 + BBR_RS16845 0.12 -1.0 1,550,258 + BBR_RS16845 0.12 +0.2 1,550,274 + BBR_RS16845 0.13 +1.5 1,550,279 + BBR_RS16845 0.14 -0.1
Or see this region's nucleotide sequence