Experiment: Recovery broth with Penicillin G 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12810 and BBR_RS12815 are separated by 306 nucleotides BBR_RS12815 and BBR_RS12820 are separated by 301 nucleotides
BBR_RS12810: BBR_RS12810 - hypothetical protein, at 622,787 to 624,178
_RS12810
BBR_RS12815: BBR_RS12815 - hypothetical protein, at 624,485 to 624,919
_RS12815
BBR_RS12820: BBR_RS12820 - hypothetical protein, at 625,221 to 626,657
_RS12820
Position (kb)
624
625 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 623.497 kb on + strand, within BBR_RS12810 at 623.572 kb on - strand, within BBR_RS12810 at 623.591 kb on + strand, within BBR_RS12810 at 623.597 kb on + strand, within BBR_RS12810 at 623.655 kb on + strand, within BBR_RS12810 at 623.673 kb on + strand, within BBR_RS12810 at 623.678 kb on - strand, within BBR_RS12810 at 623.705 kb on - strand, within BBR_RS12810 at 623.719 kb on - strand, within BBR_RS12810 at 623.731 kb on - strand, within BBR_RS12810 at 623.731 kb on - strand, within BBR_RS12810 at 623.762 kb on - strand, within BBR_RS12810 at 623.781 kb on - strand, within BBR_RS12810 at 623.788 kb on - strand, within BBR_RS12810 at 623.819 kb on + strand, within BBR_RS12810 at 623.843 kb on + strand, within BBR_RS12810 at 623.853 kb on + strand, within BBR_RS12810 at 623.861 kb on - strand, within BBR_RS12810 at 623.917 kb on + strand, within BBR_RS12810 at 623.934 kb on + strand, within BBR_RS12810 at 623.934 kb on + strand, within BBR_RS12810 at 623.934 kb on + strand, within BBR_RS12810 at 623.946 kb on + strand, within BBR_RS12810 at 623.981 kb on - strand, within BBR_RS12810 at 624.023 kb on - strand, within BBR_RS12810 at 624.035 kb on + strand, within BBR_RS12810 at 624.052 kb on + strand at 624.144 kb on - strand at 624.177 kb on - strand at 624.179 kb on + strand at 624.187 kb on - strand at 624.191 kb on + strand at 624.191 kb on + strand at 624.191 kb on + strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.199 kb on - strand at 624.227 kb on + strand at 624.233 kb on - strand at 624.233 kb on - strand at 624.243 kb on - strand at 624.257 kb on - strand at 624.283 kb on - strand at 624.285 kb on + strand at 624.293 kb on - strand at 624.361 kb on + strand at 624.369 kb on - strand at 624.369 kb on - strand at 624.373 kb on + strand at 624.455 kb on + strand at 624.455 kb on + strand at 624.455 kb on - strand at 624.492 kb on + strand at 624.493 kb on - strand at 624.519 kb on - strand at 624.519 kb on - strand at 624.520 kb on - strand at 624.528 kb on - strand at 624.557 kb on + strand, within BBR_RS12815 at 624.571 kb on + strand, within BBR_RS12815 at 624.571 kb on + strand, within BBR_RS12815 at 624.599 kb on + strand, within BBR_RS12815 at 624.632 kb on - strand, within BBR_RS12815 at 624.670 kb on + strand, within BBR_RS12815 at 624.672 kb on - strand, within BBR_RS12815 at 624.693 kb on + strand, within BBR_RS12815 at 624.696 kb on - strand, within BBR_RS12815 at 624.700 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.701 kb on - strand, within BBR_RS12815 at 624.717 kb on + strand, within BBR_RS12815 at 624.725 kb on - strand, within BBR_RS12815 at 624.735 kb on - strand, within BBR_RS12815 at 624.761 kb on + strand, within BBR_RS12815 at 624.788 kb on - strand, within BBR_RS12815 at 624.852 kb on - strand, within BBR_RS12815 at 624.859 kb on - strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.873 kb on + strand, within BBR_RS12815 at 624.881 kb on - strand at 624.881 kb on - strand at 624.899 kb on - strand at 624.928 kb on + strand at 624.936 kb on - strand at 624.936 kb on - strand at 625.094 kb on + strand at 625.095 kb on - strand at 625.095 kb on - strand at 625.100 kb on - strand at 625.117 kb on - strand at 625.137 kb on + strand at 625.148 kb on + strand at 625.168 kb on + strand at 625.168 kb on + strand at 625.221 kb on - strand at 625.257 kb on + strand at 625.347 kb on + strand at 625.353 kb on - strand at 625.400 kb on + strand, within BBR_RS12820 at 625.401 kb on + strand, within BBR_RS12820 at 625.447 kb on + strand, within BBR_RS12820 at 625.475 kb on + strand, within BBR_RS12820 at 625.505 kb on + strand, within BBR_RS12820 at 625.560 kb on - strand, within BBR_RS12820 at 625.662 kb on - strand, within BBR_RS12820 at 625.705 kb on + strand, within BBR_RS12820 at 625.708 kb on - strand, within BBR_RS12820 at 625.727 kb on - strand, within BBR_RS12820 at 625.727 kb on - strand, within BBR_RS12820 at 625.734 kb on + strand, within BBR_RS12820 at 625.734 kb on + strand, within BBR_RS12820 at 625.760 kb on + strand, within BBR_RS12820 at 625.773 kb on + strand, within BBR_RS12820 at 625.798 kb on - strand, within BBR_RS12820 at 625.806 kb on - strand, within BBR_RS12820 at 625.812 kb on - strand, within BBR_RS12820 at 625.831 kb on + strand, within BBR_RS12820 at 625.831 kb on + strand, within BBR_RS12820 at 625.876 kb on + strand, within BBR_RS12820 at 625.883 kb on + strand, within BBR_RS12820
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Penicillin G 2e-04 mM remove 623,497 + BBR_RS12810 0.51 -2.7 623,572 - BBR_RS12810 0.56 -0.6 623,591 + BBR_RS12810 0.58 -0.8 623,597 + BBR_RS12810 0.58 -2.7 623,655 + BBR_RS12810 0.62 +0.5 623,673 + BBR_RS12810 0.64 -2.3 623,678 - BBR_RS12810 0.64 -1.2 623,705 - BBR_RS12810 0.66 -2.2 623,719 - BBR_RS12810 0.67 -1.8 623,731 - BBR_RS12810 0.68 -1.8 623,731 - BBR_RS12810 0.68 -1.8 623,762 - BBR_RS12810 0.70 -1.1 623,781 - BBR_RS12810 0.71 -3.3 623,788 - BBR_RS12810 0.72 -1.0 623,819 + BBR_RS12810 0.74 -0.4 623,843 + BBR_RS12810 0.76 -0.7 623,853 + BBR_RS12810 0.77 -1.3 623,861 - BBR_RS12810 0.77 +0.8 623,917 + BBR_RS12810 0.81 +0.5 623,934 + BBR_RS12810 0.82 -2.4 623,934 + BBR_RS12810 0.82 -0.6 623,934 + BBR_RS12810 0.82 -1.1 623,946 + BBR_RS12810 0.83 -1.1 623,981 - BBR_RS12810 0.86 -1.0 624,023 - BBR_RS12810 0.89 -1.0 624,035 + BBR_RS12810 0.90 +0.3 624,052 + -0.2 624,144 - -1.9 624,177 - -0.8 624,179 + -2.0 624,187 - +3.7 624,191 + -1.3 624,191 + -2.0 624,191 + -2.8 624,199 - +3.5 624,199 - -0.1 624,199 - +3.2 624,199 - +4.1 624,227 + -1.5 624,233 - +3.4 624,233 - +0.0 624,243 - +0.9 624,257 - +1.1 624,283 - +0.6 624,285 + +1.2 624,293 - +0.9 624,361 + -0.3 624,369 - -0.5 624,369 - +1.0 624,373 + +0.2 624,455 + -0.5 624,455 + +1.1 624,455 - +0.2 624,492 + +1.1 624,493 - +0.2 624,519 - +1.0 624,519 - +1.5 624,520 - +2.0 624,528 - +1.3 624,557 + BBR_RS12815 0.17 +1.0 624,571 + BBR_RS12815 0.20 +1.0 624,571 + BBR_RS12815 0.20 +2.8 624,599 + BBR_RS12815 0.26 +1.5 624,632 - BBR_RS12815 0.34 +2.6 624,670 + BBR_RS12815 0.43 +2.6 624,672 - BBR_RS12815 0.43 +1.3 624,693 + BBR_RS12815 0.48 +2.6 624,696 - BBR_RS12815 0.49 +1.5 624,700 - BBR_RS12815 0.49 +2.3 624,701 - BBR_RS12815 0.50 +1.6 624,701 - BBR_RS12815 0.50 +1.9 624,701 - BBR_RS12815 0.50 +1.7 624,717 + BBR_RS12815 0.53 +1.3 624,725 - BBR_RS12815 0.55 +2.3 624,735 - BBR_RS12815 0.57 +2.0 624,761 + BBR_RS12815 0.63 +1.4 624,788 - BBR_RS12815 0.70 +1.8 624,852 - BBR_RS12815 0.84 +2.1 624,859 - BBR_RS12815 0.86 -3.2 624,873 + BBR_RS12815 0.89 +2.6 624,873 + BBR_RS12815 0.89 +0.1 624,873 + BBR_RS12815 0.89 +1.6 624,881 - +2.7 624,881 - +1.5 624,899 - +2.3 624,928 + +1.7 624,936 - -1.0 624,936 - +0.6 625,094 + +0.2 625,095 - +2.1 625,095 - -1.6 625,100 - -0.6 625,117 - -1.3 625,137 + +0.9 625,148 + -1.5 625,168 + +0.3 625,168 + -1.6 625,221 - +0.1 625,257 + -4.5 625,347 + -3.2 625,353 - -2.5 625,400 + BBR_RS12820 0.12 -1.3 625,401 + BBR_RS12820 0.13 -2.2 625,447 + BBR_RS12820 0.16 -1.0 625,475 + BBR_RS12820 0.18 -3.2 625,505 + BBR_RS12820 0.20 -2.7 625,560 - BBR_RS12820 0.24 -2.0 625,662 - BBR_RS12820 0.31 -1.7 625,705 + BBR_RS12820 0.34 -3.5 625,708 - BBR_RS12820 0.34 -1.7 625,727 - BBR_RS12820 0.35 -1.5 625,727 - BBR_RS12820 0.35 -1.9 625,734 + BBR_RS12820 0.36 -0.5 625,734 + BBR_RS12820 0.36 -4.4 625,760 + BBR_RS12820 0.38 -3.0 625,773 + BBR_RS12820 0.38 -3.1 625,798 - BBR_RS12820 0.40 -3.1 625,806 - BBR_RS12820 0.41 -2.0 625,812 - BBR_RS12820 0.41 -1.1 625,831 + BBR_RS12820 0.42 -2.9 625,831 + BBR_RS12820 0.42 -2.3 625,876 + BBR_RS12820 0.46 -2.0 625,883 + BBR_RS12820 0.46 -1.8
Or see this region's nucleotide sequence