Experiment: Recovery broth with Penicillin G 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12545 and BBR_RS12550 are separated by 93 nucleotides BBR_RS12550 and BBR_RS12555 are separated by 275 nucleotides
BBR_RS12545: BBR_RS12545 - DUF4012 domain-containing protein, at 571,304 to 573,064
_RS12545
BBR_RS12550: BBR_RS12550 - sugar transferase, at 573,158 to 574,876
_RS12550
BBR_RS12555: BBR_RS12555 - protein-tyrosine-phosphatase, at 575,152 to 575,757
_RS12555
Position (kb)
573
574
575 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 572.177 kb on - strand, within BBR_RS12545 at 572.304 kb on + strand, within BBR_RS12545 at 572.350 kb on + strand, within BBR_RS12545 at 572.497 kb on + strand, within BBR_RS12545 at 572.543 kb on + strand, within BBR_RS12545 at 572.543 kb on - strand, within BBR_RS12545 at 572.604 kb on + strand, within BBR_RS12545 at 572.919 kb on + strand at 572.954 kb on + strand at 572.962 kb on + strand at 572.969 kb on - strand at 573.012 kb on - strand at 573.015 kb on + strand at 573.036 kb on + strand at 573.084 kb on - strand at 573.084 kb on - strand at 573.086 kb on + strand at 573.098 kb on + strand at 573.106 kb on - strand at 573.108 kb on - strand at 573.131 kb on - strand at 573.147 kb on - strand at 573.158 kb on + strand at 573.166 kb on - strand at 573.166 kb on - strand at 573.166 kb on - strand at 573.166 kb on - strand at 573.243 kb on - strand at 573.265 kb on + strand at 573.273 kb on - strand at 573.273 kb on - strand at 573.304 kb on + strand at 573.309 kb on + strand at 573.336 kb on + strand, within BBR_RS12550 at 573.361 kb on - strand, within BBR_RS12550 at 573.361 kb on - strand, within BBR_RS12550 at 573.366 kb on - strand, within BBR_RS12550 at 573.371 kb on - strand, within BBR_RS12550 at 573.375 kb on + strand, within BBR_RS12550 at 573.418 kb on - strand, within BBR_RS12550 at 573.418 kb on - strand, within BBR_RS12550 at 573.500 kb on - strand, within BBR_RS12550 at 573.594 kb on + strand, within BBR_RS12550 at 573.622 kb on - strand, within BBR_RS12550 at 573.642 kb on + strand, within BBR_RS12550 at 573.642 kb on + strand, within BBR_RS12550 at 573.648 kb on + strand, within BBR_RS12550 at 573.648 kb on + strand, within BBR_RS12550 at 573.665 kb on - strand, within BBR_RS12550 at 573.723 kb on - strand, within BBR_RS12550 at 573.738 kb on + strand, within BBR_RS12550 at 573.764 kb on + strand, within BBR_RS12550 at 573.769 kb on + strand, within BBR_RS12550 at 573.828 kb on - strand, within BBR_RS12550 at 573.830 kb on + strand, within BBR_RS12550 at 573.833 kb on + strand, within BBR_RS12550 at 573.838 kb on - strand, within BBR_RS12550 at 573.843 kb on - strand, within BBR_RS12550 at 573.875 kb on + strand, within BBR_RS12550 at 573.875 kb on + strand, within BBR_RS12550 at 573.895 kb on + strand, within BBR_RS12550 at 573.935 kb on + strand, within BBR_RS12550 at 573.943 kb on - strand, within BBR_RS12550 at 573.961 kb on + strand, within BBR_RS12550 at 574.052 kb on + strand, within BBR_RS12550 at 574.070 kb on - strand, within BBR_RS12550 at 574.070 kb on - strand, within BBR_RS12550 at 574.070 kb on - strand, within BBR_RS12550 at 574.079 kb on + strand, within BBR_RS12550 at 574.106 kb on - strand, within BBR_RS12550 at 574.111 kb on - strand, within BBR_RS12550 at 574.114 kb on + strand, within BBR_RS12550 at 574.114 kb on + strand, within BBR_RS12550 at 574.120 kb on + strand, within BBR_RS12550 at 574.121 kb on + strand, within BBR_RS12550 at 574.122 kb on - strand, within BBR_RS12550 at 574.134 kb on - strand, within BBR_RS12550 at 574.147 kb on + strand, within BBR_RS12550 at 574.155 kb on - strand, within BBR_RS12550 at 574.240 kb on + strand, within BBR_RS12550 at 574.246 kb on - strand, within BBR_RS12550 at 574.287 kb on - strand, within BBR_RS12550 at 574.326 kb on + strand, within BBR_RS12550 at 574.345 kb on + strand, within BBR_RS12550 at 574.353 kb on - strand, within BBR_RS12550 at 574.390 kb on + strand, within BBR_RS12550 at 574.398 kb on - strand, within BBR_RS12550 at 574.398 kb on - strand, within BBR_RS12550 at 574.432 kb on - strand, within BBR_RS12550 at 574.442 kb on - strand, within BBR_RS12550 at 574.442 kb on - strand, within BBR_RS12550 at 574.461 kb on - strand, within BBR_RS12550 at 574.478 kb on + strand, within BBR_RS12550 at 574.481 kb on + strand, within BBR_RS12550 at 574.496 kb on + strand, within BBR_RS12550 at 574.496 kb on - strand, within BBR_RS12550 at 574.501 kb on + strand, within BBR_RS12550 at 574.501 kb on + strand, within BBR_RS12550 at 574.501 kb on + strand, within BBR_RS12550 at 574.509 kb on - strand, within BBR_RS12550 at 574.516 kb on + strand, within BBR_RS12550 at 574.528 kb on - strand, within BBR_RS12550 at 574.529 kb on - strand, within BBR_RS12550 at 574.534 kb on - strand, within BBR_RS12550 at 574.536 kb on - strand, within BBR_RS12550 at 574.536 kb on - strand, within BBR_RS12550 at 574.545 kb on + strand, within BBR_RS12550 at 574.577 kb on - strand, within BBR_RS12550 at 574.603 kb on - strand, within BBR_RS12550 at 574.604 kb on + strand, within BBR_RS12550 at 574.612 kb on - strand, within BBR_RS12550 at 574.642 kb on + strand, within BBR_RS12550 at 574.642 kb on + strand, within BBR_RS12550 at 574.664 kb on + strand, within BBR_RS12550 at 574.706 kb on - strand at 574.745 kb on - strand at 574.785 kb on - strand at 574.785 kb on - strand at 574.787 kb on + strand at 574.804 kb on + strand at 574.806 kb on + strand at 574.806 kb on + strand at 574.806 kb on + strand at 574.807 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.814 kb on - strand at 574.818 kb on + strand at 574.824 kb on - strand at 574.885 kb on + strand at 574.903 kb on - strand at 574.903 kb on - strand at 574.903 kb on - strand at 574.903 kb on - strand at 574.943 kb on + strand at 574.951 kb on - strand at 575.139 kb on - strand at 575.141 kb on + strand at 575.194 kb on + strand at 575.265 kb on + strand, within BBR_RS12555 at 575.265 kb on + strand, within BBR_RS12555 at 575.271 kb on + strand, within BBR_RS12555 at 575.287 kb on + strand, within BBR_RS12555 at 575.287 kb on + strand, within BBR_RS12555 at 575.292 kb on + strand, within BBR_RS12555 at 575.337 kb on + strand, within BBR_RS12555 at 575.349 kb on + strand, within BBR_RS12555 at 575.356 kb on + strand, within BBR_RS12555 at 575.430 kb on + strand, within BBR_RS12555 at 575.430 kb on + strand, within BBR_RS12555 at 575.503 kb on + strand, within BBR_RS12555 at 575.508 kb on + strand, within BBR_RS12555 at 575.523 kb on - strand, within BBR_RS12555 at 575.553 kb on - strand, within BBR_RS12555 at 575.657 kb on - strand, within BBR_RS12555 at 575.672 kb on - strand, within BBR_RS12555 at 575.686 kb on + strand, within BBR_RS12555 at 575.750 kb on - strand at 575.760 kb on - strand at 575.776 kb on + strand at 575.805 kb on + strand at 575.805 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Penicillin G 2e-04 mM remove 572,177 - BBR_RS12545 0.50 -0.1 572,304 + BBR_RS12545 0.57 +0.3 572,350 + BBR_RS12545 0.59 -1.8 572,497 + BBR_RS12545 0.68 -1.8 572,543 + BBR_RS12545 0.70 +1.5 572,543 - BBR_RS12545 0.70 -1.4 572,604 + BBR_RS12545 0.74 -2.0 572,919 + -1.7 572,954 + -0.6 572,962 + +0.3 572,969 - -1.8 573,012 - -1.2 573,015 + -1.2 573,036 + +0.4 573,084 - -0.6 573,084 - +0.6 573,086 + -0.1 573,098 + +0.1 573,106 - +0.5 573,108 - -0.1 573,131 - -0.6 573,147 - +1.0 573,158 + +0.5 573,166 - -2.0 573,166 - +0.4 573,166 - -0.9 573,166 - +1.5 573,243 - +5.6 573,265 + +5.5 573,273 - +5.7 573,273 - +6.2 573,304 + +5.7 573,309 + +5.8 573,336 + BBR_RS12550 0.10 +6.5 573,361 - BBR_RS12550 0.12 +6.1 573,361 - BBR_RS12550 0.12 +6.6 573,366 - BBR_RS12550 0.12 +5.7 573,371 - BBR_RS12550 0.12 +6.1 573,375 + BBR_RS12550 0.13 +6.2 573,418 - BBR_RS12550 0.15 +5.8 573,418 - BBR_RS12550 0.15 +6.2 573,500 - BBR_RS12550 0.20 +5.9 573,594 + BBR_RS12550 0.25 +6.1 573,622 - BBR_RS12550 0.27 +5.6 573,642 + BBR_RS12550 0.28 +5.8 573,642 + BBR_RS12550 0.28 +6.1 573,648 + BBR_RS12550 0.29 +6.2 573,648 + BBR_RS12550 0.29 +6.2 573,665 - BBR_RS12550 0.29 +6.2 573,723 - BBR_RS12550 0.33 +5.8 573,738 + BBR_RS12550 0.34 +5.6 573,764 + BBR_RS12550 0.35 +6.0 573,769 + BBR_RS12550 0.36 +5.7 573,828 - BBR_RS12550 0.39 +6.4 573,830 + BBR_RS12550 0.39 +6.0 573,833 + BBR_RS12550 0.39 +5.5 573,838 - BBR_RS12550 0.40 +5.8 573,843 - BBR_RS12550 0.40 +6.4 573,875 + BBR_RS12550 0.42 +6.3 573,875 + BBR_RS12550 0.42 +5.1 573,895 + BBR_RS12550 0.43 +5.9 573,935 + BBR_RS12550 0.45 +6.0 573,943 - BBR_RS12550 0.46 +6.0 573,961 + BBR_RS12550 0.47 +5.7 574,052 + BBR_RS12550 0.52 +6.1 574,070 - BBR_RS12550 0.53 +5.6 574,070 - BBR_RS12550 0.53 +5.7 574,070 - BBR_RS12550 0.53 +5.6 574,079 + BBR_RS12550 0.54 +5.5 574,106 - BBR_RS12550 0.55 +6.1 574,111 - BBR_RS12550 0.55 +5.7 574,114 + BBR_RS12550 0.56 +5.9 574,114 + BBR_RS12550 0.56 +5.9 574,120 + BBR_RS12550 0.56 +6.0 574,121 + BBR_RS12550 0.56 +6.2 574,122 - BBR_RS12550 0.56 +5.5 574,134 - BBR_RS12550 0.57 +5.8 574,147 + BBR_RS12550 0.58 +5.7 574,155 - BBR_RS12550 0.58 +6.4 574,240 + BBR_RS12550 0.63 +6.2 574,246 - BBR_RS12550 0.63 +6.7 574,287 - BBR_RS12550 0.66 +5.8 574,326 + BBR_RS12550 0.68 +7.3 574,345 + BBR_RS12550 0.69 +6.0 574,353 - BBR_RS12550 0.70 +5.6 574,390 + BBR_RS12550 0.72 +6.3 574,398 - BBR_RS12550 0.72 +5.8 574,398 - BBR_RS12550 0.72 +6.0 574,432 - BBR_RS12550 0.74 +5.9 574,442 - BBR_RS12550 0.75 +6.1 574,442 - BBR_RS12550 0.75 +5.4 574,461 - BBR_RS12550 0.76 +6.1 574,478 + BBR_RS12550 0.77 +5.9 574,481 + BBR_RS12550 0.77 +6.1 574,496 + BBR_RS12550 0.78 +6.1 574,496 - BBR_RS12550 0.78 +6.0 574,501 + BBR_RS12550 0.78 +5.8 574,501 + BBR_RS12550 0.78 +6.2 574,501 + BBR_RS12550 0.78 +6.3 574,509 - BBR_RS12550 0.79 +6.7 574,516 + BBR_RS12550 0.79 +5.8 574,528 - BBR_RS12550 0.80 +5.8 574,529 - BBR_RS12550 0.80 +5.8 574,534 - BBR_RS12550 0.80 +5.5 574,536 - BBR_RS12550 0.80 +6.3 574,536 - BBR_RS12550 0.80 +6.1 574,545 + BBR_RS12550 0.81 +5.5 574,577 - BBR_RS12550 0.83 +5.9 574,603 - BBR_RS12550 0.84 +6.0 574,604 + BBR_RS12550 0.84 +6.0 574,612 - BBR_RS12550 0.85 +5.9 574,642 + BBR_RS12550 0.86 +6.0 574,642 + BBR_RS12550 0.86 +6.2 574,664 + BBR_RS12550 0.88 +5.7 574,706 - +5.3 574,745 - +2.7 574,785 - +5.5 574,785 - +6.4 574,787 + +5.4 574,804 + +6.2 574,806 + +5.8 574,806 + +5.4 574,806 + +5.7 574,807 - +6.5 574,814 - +4.4 574,814 - +3.5 574,814 - +4.0 574,814 - +5.6 574,814 - +4.0 574,814 - +4.3 574,818 + +4.8 574,824 - +6.4 574,885 + +5.1 574,903 - +3.7 574,903 - +4.1 574,903 - +1.5 574,903 - +4.1 574,943 + +4.8 574,951 - +3.5 575,139 - -1.3 575,141 + +1.2 575,194 + -2.0 575,265 + BBR_RS12555 0.19 -2.8 575,265 + BBR_RS12555 0.19 -1.2 575,271 + BBR_RS12555 0.20 -3.0 575,287 + BBR_RS12555 0.22 -1.3 575,287 + BBR_RS12555 0.22 -2.6 575,292 + BBR_RS12555 0.23 -1.9 575,337 + BBR_RS12555 0.31 -0.6 575,349 + BBR_RS12555 0.33 -0.3 575,356 + BBR_RS12555 0.34 -4.0 575,430 + BBR_RS12555 0.46 -3.3 575,430 + BBR_RS12555 0.46 -2.4 575,503 + BBR_RS12555 0.58 -2.4 575,508 + BBR_RS12555 0.59 -1.7 575,523 - BBR_RS12555 0.61 -2.2 575,553 - BBR_RS12555 0.66 -1.6 575,657 - BBR_RS12555 0.83 -2.8 575,672 - BBR_RS12555 0.86 +2.1 575,686 + BBR_RS12555 0.88 -2.0 575,750 - +0.2 575,760 - +1.7 575,776 + +0.4 575,805 + +1.1 575,805 + +1.1
Or see this region's nucleotide sequence