Experiment: Recovery broth with Penicillin G 2e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11620 and BBR_RS11625 are separated by 637 nucleotides BBR_RS11625 and BBR_RS11630 are separated by 633 nucleotides
BBR_RS11620: BBR_RS11620 - Two-component sensor protein, at 362,776 to 363,942
_RS11620
BBR_RS11625: BBR_RS11625 - transposase, at 364,580 to 364,801
_RS11625
BBR_RS11630: BBR_RS11630 - MFS transporter, at 365,435 to 366,640
_RS11630
Position (kb) 364
365 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 363.582 kb on + strand, within BBR_RS11620 at 363.582 kb on + strand, within BBR_RS11620 at 363.590 kb on - strand, within BBR_RS11620 at 363.590 kb on - strand, within BBR_RS11620 at 363.590 kb on - strand, within BBR_RS11620 at 363.590 kb on - strand, within BBR_RS11620 at 363.590 kb on - strand, within BBR_RS11620 at 363.590 kb on - strand, within BBR_RS11620 at 363.642 kb on - strand, within BBR_RS11620 at 363.657 kb on - strand, within BBR_RS11620 at 363.685 kb on - strand, within BBR_RS11620 at 363.687 kb on + strand, within BBR_RS11620 at 363.688 kb on + strand, within BBR_RS11620 at 363.688 kb on + strand, within BBR_RS11620 at 363.688 kb on + strand, within BBR_RS11620 at 363.688 kb on + strand, within BBR_RS11620 at 363.689 kb on + strand, within BBR_RS11620 at 363.751 kb on - strand, within BBR_RS11620 at 363.815 kb on - strand, within BBR_RS11620 at 363.826 kb on - strand at 363.860 kb on - strand at 363.991 kb on + strand at 363.999 kb on - strand at 364.009 kb on - strand at 364.064 kb on - strand at 364.088 kb on - strand at 364.091 kb on + strand at 364.094 kb on + strand at 364.099 kb on - strand at 364.102 kb on - strand at 364.102 kb on - strand at 364.108 kb on + strand at 364.121 kb on - strand at 364.166 kb on - strand at 364.171 kb on - strand at 364.188 kb on + strand at 364.188 kb on + strand at 364.188 kb on + strand at 364.188 kb on + strand at 364.191 kb on + strand at 364.204 kb on + strand at 364.226 kb on + strand at 364.236 kb on + strand at 364.248 kb on + strand at 364.254 kb on + strand at 364.254 kb on + strand at 364.259 kb on + strand at 364.259 kb on + strand at 364.264 kb on + strand at 364.267 kb on - strand at 364.272 kb on - strand at 364.273 kb on + strand at 364.309 kb on + strand at 364.312 kb on - strand at 364.317 kb on - strand at 364.325 kb on + strand at 364.348 kb on - strand at 364.350 kb on + strand at 364.358 kb on - strand at 364.414 kb on - strand at 364.414 kb on - strand at 364.430 kb on + strand at 364.437 kb on + strand at 364.466 kb on + strand at 364.483 kb on - strand at 364.485 kb on - strand at 364.499 kb on - strand at 364.539 kb on + strand at 364.548 kb on + strand at 364.548 kb on + strand at 364.548 kb on + strand at 364.564 kb on - strand at 364.565 kb on - strand at 364.599 kb on + strand at 364.609 kb on + strand, within BBR_RS11625 at 364.609 kb on + strand, within BBR_RS11625 at 364.614 kb on + strand, within BBR_RS11625 at 364.614 kb on + strand, within BBR_RS11625 at 364.614 kb on + strand, within BBR_RS11625 at 364.639 kb on + strand, within BBR_RS11625 at 364.640 kb on + strand, within BBR_RS11625 at 364.640 kb on + strand, within BBR_RS11625 at 364.640 kb on + strand, within BBR_RS11625 at 364.643 kb on - strand, within BBR_RS11625 at 364.643 kb on - strand, within BBR_RS11625 at 364.648 kb on - strand, within BBR_RS11625 at 364.688 kb on - strand, within BBR_RS11625 at 364.694 kb on - strand, within BBR_RS11625 at 364.710 kb on + strand, within BBR_RS11625 at 364.710 kb on + strand, within BBR_RS11625 at 364.719 kb on + strand, within BBR_RS11625 at 364.769 kb on + strand, within BBR_RS11625 at 364.770 kb on + strand, within BBR_RS11625 at 364.777 kb on - strand, within BBR_RS11625 at 364.777 kb on - strand, within BBR_RS11625 at 364.778 kb on - strand, within BBR_RS11625 at 364.778 kb on - strand, within BBR_RS11625 at 364.859 kb on + strand at 364.859 kb on + strand at 364.905 kb on + strand at 364.906 kb on + strand at 364.909 kb on + strand at 364.909 kb on + strand at 364.913 kb on - strand at 364.913 kb on - strand at 364.925 kb on + strand at 364.925 kb on + strand at 364.959 kb on + strand at 364.981 kb on - strand at 365.007 kb on + strand at 365.064 kb on + strand at 365.064 kb on + strand at 365.065 kb on + strand at 365.072 kb on - strand at 365.072 kb on - strand at 365.089 kb on + strand at 365.089 kb on + strand at 365.091 kb on + strand at 365.091 kb on + strand at 365.091 kb on + strand at 365.141 kb on + strand at 365.141 kb on + strand at 365.141 kb on + strand at 365.141 kb on + strand at 365.141 kb on - strand at 365.144 kb on - strand at 365.149 kb on - strand at 365.149 kb on - strand at 365.149 kb on - strand at 365.159 kb on + strand at 365.159 kb on + strand at 365.159 kb on + strand at 365.167 kb on - strand at 365.167 kb on - strand at 365.167 kb on - strand at 365.167 kb on - strand at 365.167 kb on - strand at 365.167 kb on - strand at 365.172 kb on - strand at 365.179 kb on + strand at 365.181 kb on + strand at 365.185 kb on - strand at 365.187 kb on - strand at 365.187 kb on - strand at 365.190 kb on + strand at 365.197 kb on + strand at 365.198 kb on - strand at 365.214 kb on + strand at 365.214 kb on + strand at 365.236 kb on + strand at 365.236 kb on + strand at 365.247 kb on + strand at 365.247 kb on + strand at 365.252 kb on + strand at 365.255 kb on - strand at 365.260 kb on - strand at 365.260 kb on - strand at 365.287 kb on + strand at 365.298 kb on - strand at 365.299 kb on - strand at 365.402 kb on - strand at 365.402 kb on - strand at 365.405 kb on + strand at 365.415 kb on + strand at 365.425 kb on + strand at 365.425 kb on + strand at 365.433 kb on - strand at 365.443 kb on + strand at 365.443 kb on + strand at 365.443 kb on + strand at 365.443 kb on + strand at 365.448 kb on + strand at 365.459 kb on - strand at 365.510 kb on - strand at 365.510 kb on - strand at 365.516 kb on + strand at 365.556 kb on - strand, within BBR_RS11630 at 365.560 kb on - strand, within BBR_RS11630 at 365.613 kb on + strand, within BBR_RS11630 at 365.618 kb on + strand, within BBR_RS11630 at 365.646 kb on + strand, within BBR_RS11630 at 365.690 kb on + strand, within BBR_RS11630 at 365.690 kb on + strand, within BBR_RS11630 at 365.690 kb on + strand, within BBR_RS11630 at 365.698 kb on - strand, within BBR_RS11630 at 365.698 kb on - strand, within BBR_RS11630 at 365.699 kb on + strand, within BBR_RS11630 at 365.708 kb on + strand, within BBR_RS11630 at 365.708 kb on + strand, within BBR_RS11630 at 365.713 kb on + strand, within BBR_RS11630 at 365.716 kb on - strand, within BBR_RS11630 at 365.727 kb on - strand, within BBR_RS11630 at 365.727 kb on - strand, within BBR_RS11630 at 365.738 kb on + strand, within BBR_RS11630 at 365.752 kb on + strand, within BBR_RS11630 at 365.757 kb on + strand, within BBR_RS11630 at 365.757 kb on + strand, within BBR_RS11630 at 365.757 kb on + strand, within BBR_RS11630 at 365.757 kb on + strand, within BBR_RS11630 at 365.760 kb on - strand, within BBR_RS11630 at 365.760 kb on - strand, within BBR_RS11630 at 365.762 kb on + strand, within BBR_RS11630 at 365.762 kb on + strand, within BBR_RS11630 at 365.763 kb on - strand, within BBR_RS11630 at 365.765 kb on - strand, within BBR_RS11630 at 365.765 kb on - strand, within BBR_RS11630 at 365.765 kb on - strand, within BBR_RS11630 at 365.765 kb on - strand, within BBR_RS11630 at 365.765 kb on - strand, within BBR_RS11630 at 365.766 kb on + strand, within BBR_RS11630 at 365.766 kb on + strand, within BBR_RS11630 at 365.770 kb on - strand, within BBR_RS11630 at 365.777 kb on + strand, within BBR_RS11630 at 365.777 kb on + strand, within BBR_RS11630 at 365.777 kb on + strand, within BBR_RS11630 at 365.777 kb on + strand, within BBR_RS11630 at 365.780 kb on - strand, within BBR_RS11630 at 365.785 kb on - strand, within BBR_RS11630 at 365.785 kb on - strand, within BBR_RS11630 at 365.785 kb on - strand, within BBR_RS11630 at 365.786 kb on - strand, within BBR_RS11630 at 365.786 kb on - strand, within BBR_RS11630
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Penicillin G 2e-04 mM remove 363,582 + BBR_RS11620 0.69 +0.6 363,582 + BBR_RS11620 0.69 +0.3 363,590 - BBR_RS11620 0.70 -0.0 363,590 - BBR_RS11620 0.70 +0.3 363,590 - BBR_RS11620 0.70 +0.9 363,590 - BBR_RS11620 0.70 -0.7 363,590 - BBR_RS11620 0.70 +0.8 363,590 - BBR_RS11620 0.70 +0.7 363,642 - BBR_RS11620 0.74 -1.2 363,657 - BBR_RS11620 0.75 -0.2 363,685 - BBR_RS11620 0.78 +0.7 363,687 + BBR_RS11620 0.78 +0.3 363,688 + BBR_RS11620 0.78 -0.7 363,688 + BBR_RS11620 0.78 -0.3 363,688 + BBR_RS11620 0.78 +1.5 363,688 + BBR_RS11620 0.78 +0.3 363,689 + BBR_RS11620 0.78 +0.2 363,751 - BBR_RS11620 0.84 -0.2 363,815 - BBR_RS11620 0.89 +0.3 363,826 - +0.1 363,860 - -1.3 363,991 + -2.5 363,999 - +1.6 364,009 - +1.2 364,064 - -0.3 364,088 - -0.5 364,091 + +0.2 364,094 + +0.3 364,099 - -0.4 364,102 - -0.2 364,102 - -1.6 364,108 + +1.6 364,121 - +2.1 364,166 - -0.0 364,171 - +0.7 364,188 + -0.5 364,188 + +1.0 364,188 + -0.7 364,188 + +1.1 364,191 + +1.6 364,204 + +0.2 364,226 + +0.2 364,236 + -0.5 364,248 + +0.9 364,254 + +0.1 364,254 + +0.5 364,259 + -0.2 364,259 + -1.6 364,264 + +0.1 364,267 - +0.0 364,272 - -1.2 364,273 + +2.1 364,309 + +0.7 364,312 - +0.4 364,317 - +2.2 364,325 + +2.8 364,348 - -0.3 364,350 + +1.6 364,358 - -0.2 364,414 - -0.1 364,414 - -1.4 364,430 + +0.6 364,437 + +0.4 364,466 + -1.6 364,483 - +0.6 364,485 - -0.3 364,499 - +1.1 364,539 + +0.5 364,548 + -2.2 364,548 + -1.0 364,548 + -0.2 364,564 - -0.2 364,565 - -0.5 364,599 + -1.3 364,609 + BBR_RS11625 0.13 +0.1 364,609 + BBR_RS11625 0.13 -0.2 364,614 + BBR_RS11625 0.15 +1.0 364,614 + BBR_RS11625 0.15 -0.4 364,614 + BBR_RS11625 0.15 +1.4 364,639 + BBR_RS11625 0.27 +0.3 364,640 + BBR_RS11625 0.27 +1.6 364,640 + BBR_RS11625 0.27 +1.2 364,640 + BBR_RS11625 0.27 +0.9 364,643 - BBR_RS11625 0.28 +0.7 364,643 - BBR_RS11625 0.28 +0.6 364,648 - BBR_RS11625 0.31 +3.4 364,688 - BBR_RS11625 0.49 +1.3 364,694 - BBR_RS11625 0.51 -0.6 364,710 + BBR_RS11625 0.59 -0.6 364,710 + BBR_RS11625 0.59 -0.0 364,719 + BBR_RS11625 0.63 +0.0 364,769 + BBR_RS11625 0.85 -0.6 364,770 + BBR_RS11625 0.86 +0.0 364,777 - BBR_RS11625 0.89 -0.2 364,777 - BBR_RS11625 0.89 +0.6 364,778 - BBR_RS11625 0.89 +0.0 364,778 - BBR_RS11625 0.89 +1.1 364,859 + +0.7 364,859 + +1.2 364,905 + -0.6 364,906 + +0.5 364,909 + +0.4 364,909 + -1.4 364,913 - +2.2 364,913 - -0.2 364,925 + +0.1 364,925 + +0.3 364,959 + -1.0 364,981 - -0.7 365,007 + +0.2 365,064 + +0.3 365,064 + +0.1 365,065 + +0.1 365,072 - -1.3 365,072 - +1.9 365,089 + -1.7 365,089 + -1.6 365,091 + -1.0 365,091 + +0.2 365,091 + +0.2 365,141 + -0.5 365,141 + -1.8 365,141 + -0.5 365,141 + +0.1 365,141 - -0.7 365,144 - +1.4 365,149 - -0.8 365,149 - +5.8 365,149 - +0.3 365,159 + -0.6 365,159 + -0.7 365,159 + -0.1 365,167 - -0.4 365,167 - +1.4 365,167 - +0.1 365,167 - -0.0 365,167 - +1.5 365,167 - +0.1 365,172 - +0.2 365,179 + -0.7 365,181 + -0.3 365,185 - +0.3 365,187 - -1.1 365,187 - +0.6 365,190 + -0.2 365,197 + +1.8 365,198 - -1.1 365,214 + -1.0 365,214 + -0.2 365,236 + +1.0 365,236 + +0.1 365,247 + -0.9 365,247 + -0.3 365,252 + +0.1 365,255 - -1.1 365,260 - -0.4 365,260 - -0.4 365,287 + +0.1 365,298 - +1.7 365,299 - -1.0 365,402 - +1.2 365,402 - +0.3 365,405 + -0.3 365,415 + +1.0 365,425 + -1.3 365,425 + -2.1 365,433 - +1.5 365,443 + +0.4 365,443 + -0.3 365,443 + +0.1 365,443 + -0.3 365,448 + +0.3 365,459 - +0.1 365,510 - -0.4 365,510 - -0.1 365,516 + -0.4 365,556 - BBR_RS11630 0.10 +0.8 365,560 - BBR_RS11630 0.10 -1.6 365,613 + BBR_RS11630 0.15 -0.3 365,618 + BBR_RS11630 0.15 +1.3 365,646 + BBR_RS11630 0.17 +0.1 365,690 + BBR_RS11630 0.21 -1.7 365,690 + BBR_RS11630 0.21 -0.0 365,690 + BBR_RS11630 0.21 +1.6 365,698 - BBR_RS11630 0.22 -0.1 365,698 - BBR_RS11630 0.22 +1.1 365,699 + BBR_RS11630 0.22 +1.1 365,708 + BBR_RS11630 0.23 -0.1 365,708 + BBR_RS11630 0.23 +0.8 365,713 + BBR_RS11630 0.23 +0.9 365,716 - BBR_RS11630 0.23 +1.2 365,727 - BBR_RS11630 0.24 -1.2 365,727 - BBR_RS11630 0.24 -0.4 365,738 + BBR_RS11630 0.25 +3.0 365,752 + BBR_RS11630 0.26 +1.2 365,757 + BBR_RS11630 0.27 +0.8 365,757 + BBR_RS11630 0.27 +0.5 365,757 + BBR_RS11630 0.27 +0.2 365,757 + BBR_RS11630 0.27 -0.1 365,760 - BBR_RS11630 0.27 +0.3 365,760 - BBR_RS11630 0.27 -0.2 365,762 + BBR_RS11630 0.27 -0.1 365,762 + BBR_RS11630 0.27 -0.7 365,763 - BBR_RS11630 0.27 +1.8 365,765 - BBR_RS11630 0.27 +0.6 365,765 - BBR_RS11630 0.27 +0.0 365,765 - BBR_RS11630 0.27 -0.5 365,765 - BBR_RS11630 0.27 +1.3 365,765 - BBR_RS11630 0.27 -0.3 365,766 + BBR_RS11630 0.27 +0.2 365,766 + BBR_RS11630 0.27 +1.1 365,770 - BBR_RS11630 0.28 -0.1 365,777 + BBR_RS11630 0.28 -0.1 365,777 + BBR_RS11630 0.28 -1.2 365,777 + BBR_RS11630 0.28 -0.2 365,777 + BBR_RS11630 0.28 +3.7 365,780 - BBR_RS11630 0.29 +3.1 365,785 - BBR_RS11630 0.29 +0.8 365,785 - BBR_RS11630 0.29 +2.6 365,785 - BBR_RS11630 0.29 -1.3 365,786 - BBR_RS11630 0.29 +1.3 365,786 - BBR_RS11630 0.29 +0.9
Or see this region's nucleotide sequence