Experiment: MoYLS4 with Bacitracin 1.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3138 and DvMF_3139 are separated by 6 nucleotides DvMF_3139 and DvMF_3140 are separated by 335 nucleotides DvMF_3140 and DvMF_3141 are separated by 473 nucleotides
DvMF_3138: DvMF_3138 - cobalt ABC transporter, inner membrane subunit CbiQ (RefSeq), at 3,957,986 to 3,958,783
_3138
DvMF_3139: DvMF_3139 - cobalt transport protein CbiM (RefSeq), at 3,958,790 to 3,959,404
_3139
DvMF_3140: DvMF_3140 - Citrate transporter (RefSeq), at 3,959,740 to 3,961,173
_3140
DvMF_3141: DvMF_3141 - tRNA guanosine-2'-O-methyltransferase (RefSeq), at 3,961,647 to 3,962,210
_3141
Position (kb)
3959
3960
3961
3962 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3958.832 kb on + strand at 3959.000 kb on + strand, within DvMF_3139 at 3959.083 kb on - strand, within DvMF_3139 at 3959.124 kb on + strand, within DvMF_3139 at 3959.399 kb on + strand at 3959.474 kb on + strand at 3959.629 kb on + strand at 3959.652 kb on + strand at 3959.750 kb on - strand at 3961.566 kb on - strand at 3961.693 kb on + strand at 3961.779 kb on - strand, within DvMF_3141 at 3961.789 kb on - strand, within DvMF_3141 at 3962.067 kb on + strand, within DvMF_3141
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1.4 mg/ml remove 3,958,832 + -0.4 3,959,000 + DvMF_3139 0.34 +0.1 3,959,083 - DvMF_3139 0.48 +0.3 3,959,124 + DvMF_3139 0.54 -0.1 3,959,399 + -0.6 3,959,474 + +0.3 3,959,629 + -0.9 3,959,652 + -0.7 3,959,750 - -0.3 3,961,566 - -1.3 3,961,693 + -2.4 3,961,779 - DvMF_3141 0.23 -2.8 3,961,789 - DvMF_3141 0.25 -2.5 3,962,067 + DvMF_3141 0.74 -2.1
Or see this region's nucleotide sequence