Experiment: MoYLS4 with Bacitracin 1.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3039 and DvMF_3040 are separated by 443 nucleotides DvMF_3040 and DvMF_3041 overlap by 4 nucleotides DvMF_3041 and DvMF_3042 are separated by 104 nucleotides
DvMF_3039: DvMF_3039 - peptidase M24 (RefSeq), at 3,836,186 to 3,837,445
_3039
DvMF_3040: DvMF_3040 - coenzyme A binding protein (RefSeq), at 3,837,889 to 3,838,311
_3040
DvMF_3041: DvMF_3041 - protein of unknown function UPF0153 (RefSeq), at 3,838,308 to 3,838,835
_3041
DvMF_3042: DvMF_3042 - heat shock protein DnaJ domain protein (RefSeq), at 3,838,940 to 3,839,908
_3042
Position (kb)
3838
3839 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3837.429 kb on + strand at 3837.491 kb on + strand at 3837.499 kb on - strand at 3837.499 kb on - strand at 3837.546 kb on - strand at 3837.670 kb on - strand at 3837.943 kb on - strand at 3838.586 kb on + strand, within DvMF_3041 at 3838.594 kb on - strand, within DvMF_3041 at 3839.067 kb on - strand, within DvMF_3042 at 3839.307 kb on - strand, within DvMF_3042 at 3839.601 kb on + strand, within DvMF_3042 at 3839.609 kb on - strand, within DvMF_3042 at 3839.691 kb on - strand, within DvMF_3042 at 3839.765 kb on - strand, within DvMF_3042
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1.4 mg/ml remove 3,837,429 + -0.7 3,837,491 + +0.1 3,837,499 - -0.4 3,837,499 - -0.4 3,837,546 - +1.2 3,837,670 - -0.8 3,837,943 - +5.3 3,838,586 + DvMF_3041 0.53 -0.1 3,838,594 - DvMF_3041 0.54 +0.6 3,839,067 - DvMF_3042 0.13 -0.3 3,839,307 - DvMF_3042 0.38 +1.1 3,839,601 + DvMF_3042 0.68 -0.6 3,839,609 - DvMF_3042 0.69 -0.9 3,839,691 - DvMF_3042 0.78 -0.5 3,839,765 - DvMF_3042 0.85 -0.3
Or see this region's nucleotide sequence