Experiment: MoYLS4 with Bacitracin 1.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2319 and DvMF_R0067 are separated by 206 nucleotides DvMF_R0067 and DvMF_2320 are separated by 200 nucleotides DvMF_2320 and DvMF_2321 are separated by 357 nucleotides
DvMF_2319: DvMF_2319 - phosphoesterase, Icc protein-like protein (RefSeq), at 2,918,195 to 2,918,872
_2319
DvMF_R0067: DvMF_R0067 - tRNA-Met (RefSeq), at 2,919,079 to 2,919,155
_R0067
DvMF_2320: DvMF_2320 - 6-phosphogluconate dehydrogenase NAD-binding (RefSeq), at 2,919,356 to 2,920,219
_2320
DvMF_2321: DvMF_2321 - hypothetical protein (RefSeq), at 2,920,577 to 2,921,281
_2321
Position (kb)
2919
2920
2921 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2918.411 kb on - strand, within DvMF_2319 at 2918.411 kb on - strand, within DvMF_2319 at 2918.483 kb on - strand, within DvMF_2319 at 2918.513 kb on - strand, within DvMF_2319 at 2918.650 kb on + strand, within DvMF_2319 at 2918.722 kb on + strand, within DvMF_2319 at 2918.757 kb on + strand, within DvMF_2319 at 2919.321 kb on - strand at 2919.332 kb on - strand at 2919.417 kb on - strand at 2919.418 kb on - strand at 2919.422 kb on + strand at 2919.783 kb on + strand, within DvMF_2320 at 2919.798 kb on + strand, within DvMF_2320 at 2919.936 kb on - strand, within DvMF_2320 at 2920.102 kb on - strand, within DvMF_2320 at 2920.413 kb on + strand at 2920.432 kb on + strand at 2920.500 kb on - strand at 2920.528 kb on - strand at 2920.661 kb on - strand, within DvMF_2321 at 2920.833 kb on - strand, within DvMF_2321 at 2921.028 kb on + strand, within DvMF_2321 at 2921.094 kb on - strand, within DvMF_2321 at 2921.123 kb on + strand, within DvMF_2321
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1.4 mg/ml remove 2,918,411 - DvMF_2319 0.32 -1.3 2,918,411 - DvMF_2319 0.32 +0.4 2,918,483 - DvMF_2319 0.42 -1.3 2,918,513 - DvMF_2319 0.47 -0.7 2,918,650 + DvMF_2319 0.67 +0.1 2,918,722 + DvMF_2319 0.78 -0.6 2,918,757 + DvMF_2319 0.83 +0.0 2,919,321 - -0.7 2,919,332 - -0.8 2,919,417 - -1.4 2,919,418 - +0.4 2,919,422 + -1.2 2,919,783 + DvMF_2320 0.49 -1.0 2,919,798 + DvMF_2320 0.51 +3.3 2,919,936 - DvMF_2320 0.67 -1.2 2,920,102 - DvMF_2320 0.86 +1.0 2,920,413 + -0.9 2,920,432 + -1.2 2,920,500 - -1.1 2,920,528 - -1.0 2,920,661 - DvMF_2321 0.12 -1.1 2,920,833 - DvMF_2321 0.36 -1.1 2,921,028 + DvMF_2321 0.64 -0.6 2,921,094 - DvMF_2321 0.73 +0.2 2,921,123 + DvMF_2321 0.77 -2.6
Or see this region's nucleotide sequence