Experiment: MoYLS4 with Bacitracin 1.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2211 and DvMF_2212 are separated by 81 nucleotides DvMF_2212 and DvMF_2213 are separated by 26 nucleotides DvMF_2213 and DvMF_2214 are separated by 103 nucleotides DvMF_2214 and fliP are separated by 139 nucleotides
DvMF_2211: DvMF_2211 - OmpA/MotB domain protein (RefSeq), at 2,764,982 to 2,765,917
_2211
DvMF_2212: DvMF_2212 - flagellar basal body-associated protein FliL (RefSeq), at 2,765,999 to 2,766,514
_2212
DvMF_2213: DvMF_2213 - flagellar motor switch protein FliN (RefSeq), at 2,766,541 to 2,767,083
_2213
DvMF_2214: DvMF_2214 - flagellar biosynthesis protein FliO (RefSeq), at 2,767,187 to 2,767,762
_2214
DvMF_2215: fliP - flagellar biosynthesis protein FliP (RefSeq), at 2,767,902 to 2,768,783
fliP
Position (kb)
2766
2767
2768 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2765.550 kb on - strand, within DvMF_2211 at 2765.679 kb on - strand, within DvMF_2211 at 2765.823 kb on - strand, within DvMF_2211 at 2765.868 kb on + strand at 2765.980 kb on - strand at 2766.054 kb on + strand, within DvMF_2212 at 2766.172 kb on + strand, within DvMF_2212 at 2766.468 kb on + strand at 2766.704 kb on + strand, within DvMF_2213 at 2766.704 kb on + strand, within DvMF_2213 at 2766.712 kb on - strand, within DvMF_2213 at 2766.914 kb on - strand, within DvMF_2213 at 2766.916 kb on + strand, within DvMF_2213 at 2766.996 kb on + strand, within DvMF_2213 at 2767.267 kb on - strand, within DvMF_2214 at 2767.427 kb on - strand, within DvMF_2214 at 2767.529 kb on - strand, within DvMF_2214 at 2767.854 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1.4 mg/ml remove 2,765,550 - DvMF_2211 0.61 -0.5 2,765,679 - DvMF_2211 0.74 -0.9 2,765,823 - DvMF_2211 0.90 -0.7 2,765,868 + -0.1 2,765,980 - -0.9 2,766,054 + DvMF_2212 0.11 +1.0 2,766,172 + DvMF_2212 0.34 +1.1 2,766,468 + +1.2 2,766,704 + DvMF_2213 0.30 -0.6 2,766,704 + DvMF_2213 0.30 +1.9 2,766,712 - DvMF_2213 0.31 +2.0 2,766,914 - DvMF_2213 0.69 +1.4 2,766,916 + DvMF_2213 0.69 +1.7 2,766,996 + DvMF_2213 0.84 +2.9 2,767,267 - DvMF_2214 0.14 -0.3 2,767,427 - DvMF_2214 0.42 +0.7 2,767,529 - DvMF_2214 0.59 +0.8 2,767,854 + +2.7
Or see this region's nucleotide sequence