Experiment: MoYLS4 with Bacitracin 1.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1970 and DvMF_R0052 are separated by 445 nucleotides DvMF_R0052 and DvMF_1971 are separated by 155 nucleotides DvMF_1971 and DvMF_1972 are separated by 99 nucleotides DvMF_1972 and DvMF_1973 overlap by 4 nucleotides
DvMF_1970: DvMF_1970 - hypothetical protein (RefSeq), at 2,435,371 to 2,435,865
_1970
DvMF_R0052: DvMF_R0052 - tRNA-Leu (RefSeq), at 2,436,311 to 2,436,397
_R0052
DvMF_1971: DvMF_1971 - CRISPR-associated helicase Cas3 (RefSeq), at 2,436,553 to 2,438,775
_1971
DvMF_1972: DvMF_1972 - CRISPR-associated protein Cas5 family (RefSeq), at 2,438,875 to 2,439,540
_1972
DvMF_1973: DvMF_1973 - CRISPR-associated protein, Csd1 family (RefSeq), at 2,439,537 to 2,441,426
_1973
Position (kb)
2436
2437
2438
2439 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2435.596 kb on - strand, within DvMF_1970 at 2435.831 kb on - strand at 2435.885 kb on + strand at 2435.885 kb on + strand at 2435.895 kb on + strand at 2435.913 kb on - strand at 2436.095 kb on + strand at 2436.426 kb on + strand at 2436.434 kb on - strand at 2436.443 kb on - strand at 2436.470 kb on + strand at 2436.478 kb on - strand at 2436.762 kb on + strand at 2436.773 kb on + strand at 2436.845 kb on + strand, within DvMF_1971 at 2436.853 kb on - strand, within DvMF_1971 at 2436.892 kb on - strand, within DvMF_1971 at 2436.977 kb on - strand, within DvMF_1971 at 2437.159 kb on + strand, within DvMF_1971 at 2437.238 kb on + strand, within DvMF_1971 at 2437.281 kb on - strand, within DvMF_1971 at 2437.325 kb on + strand, within DvMF_1971 at 2437.335 kb on + strand, within DvMF_1971 at 2437.369 kb on + strand, within DvMF_1971 at 2437.414 kb on + strand, within DvMF_1971 at 2437.504 kb on - strand, within DvMF_1971 at 2437.510 kb on + strand, within DvMF_1971 at 2437.510 kb on + strand, within DvMF_1971 at 2437.549 kb on - strand, within DvMF_1971 at 2437.655 kb on - strand, within DvMF_1971 at 2437.828 kb on - strand, within DvMF_1971 at 2437.868 kb on - strand, within DvMF_1971 at 2437.888 kb on - strand, within DvMF_1971 at 2437.893 kb on - strand, within DvMF_1971 at 2437.944 kb on + strand, within DvMF_1971 at 2438.029 kb on - strand, within DvMF_1971 at 2438.106 kb on - strand, within DvMF_1971 at 2438.263 kb on + strand, within DvMF_1971 at 2438.271 kb on - strand, within DvMF_1971 at 2438.430 kb on - strand, within DvMF_1971 at 2438.580 kb on - strand at 2438.617 kb on + strand at 2438.657 kb on - strand at 2438.704 kb on - strand at 2438.711 kb on - strand at 2438.725 kb on - strand at 2438.738 kb on + strand at 2438.832 kb on + strand at 2438.908 kb on + strand at 2438.934 kb on + strand at 2439.086 kb on - strand, within DvMF_1972 at 2439.188 kb on + strand, within DvMF_1972 at 2439.202 kb on + strand, within DvMF_1972 at 2439.360 kb on + strand, within DvMF_1972 at 2439.368 kb on - strand, within DvMF_1972 at 2439.529 kb on - strand at 2439.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1.4 mg/ml remove 2,435,596 - DvMF_1970 0.45 +0.5 2,435,831 - -1.2 2,435,885 + -2.8 2,435,885 + +4.2 2,435,895 + -0.6 2,435,913 - +0.8 2,436,095 + -0.6 2,436,426 + +0.1 2,436,434 - +3.5 2,436,443 - -0.4 2,436,470 + -1.2 2,436,478 - -2.0 2,436,762 + +0.2 2,436,773 + -0.9 2,436,845 + DvMF_1971 0.13 -0.2 2,436,853 - DvMF_1971 0.13 -0.2 2,436,892 - DvMF_1971 0.15 -1.7 2,436,977 - DvMF_1971 0.19 -1.2 2,437,159 + DvMF_1971 0.27 -0.8 2,437,238 + DvMF_1971 0.31 -0.6 2,437,281 - DvMF_1971 0.33 -1.2 2,437,325 + DvMF_1971 0.35 -2.0 2,437,335 + DvMF_1971 0.35 -0.5 2,437,369 + DvMF_1971 0.37 +0.4 2,437,414 + DvMF_1971 0.39 -0.2 2,437,504 - DvMF_1971 0.43 +0.5 2,437,510 + DvMF_1971 0.43 +1.5 2,437,510 + DvMF_1971 0.43 -1.3 2,437,549 - DvMF_1971 0.45 -0.8 2,437,655 - DvMF_1971 0.50 -0.7 2,437,828 - DvMF_1971 0.57 -0.6 2,437,868 - DvMF_1971 0.59 -0.0 2,437,888 - DvMF_1971 0.60 -0.8 2,437,893 - DvMF_1971 0.60 -1.5 2,437,944 + DvMF_1971 0.63 -0.6 2,438,029 - DvMF_1971 0.66 -0.9 2,438,106 - DvMF_1971 0.70 -0.7 2,438,263 + DvMF_1971 0.77 -0.5 2,438,271 - DvMF_1971 0.77 -1.2 2,438,430 - DvMF_1971 0.84 -0.4 2,438,580 - -1.1 2,438,617 + -0.6 2,438,657 - -0.4 2,438,704 - -0.6 2,438,711 - +0.0 2,438,725 - -2.4 2,438,738 + -0.8 2,438,832 + -0.5 2,438,908 + +0.5 2,438,934 + -1.6 2,439,086 - DvMF_1972 0.32 -2.7 2,439,188 + DvMF_1972 0.47 -0.4 2,439,202 + DvMF_1972 0.49 -1.4 2,439,360 + DvMF_1972 0.73 -0.8 2,439,368 - DvMF_1972 0.74 -0.8 2,439,529 - -1.1 2,439,617 - -1.0
Or see this region's nucleotide sequence