Experiment: MoYLS4 with Bacitracin 1.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0259 and DvMF_R0015 are separated by 190 nucleotides DvMF_R0015 and DvMF_0260 are separated by 120 nucleotides DvMF_0260 and DvMF_0261 are separated by 279 nucleotides
DvMF_0259: DvMF_0259 - protein of unknown function DUF34 (RefSeq), at 296,363 to 297,397
_0259
DvMF_R0015: DvMF_R0015 - tRNA-Met (RefSeq), at 297,588 to 297,663
_R0015
DvMF_0260: DvMF_0260 - Thioredoxin-disulfide reductase (RefSeq), at 297,784 to 298,740
_0260
DvMF_0261: DvMF_0261 - putative chemotaxis phosphatase, CheZ (RefSeq), at 299,020 to 299,766
_0261
Position (kb) 297
298
299 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 297.092 kb on - strand, within DvMF_0259 at 297.118 kb on + strand, within DvMF_0259 at 297.441 kb on + strand at 297.485 kb on - strand at 297.832 kb on + strand at 297.837 kb on + strand at 297.840 kb on - strand at 297.965 kb on + strand, within DvMF_0260 at 298.159 kb on + strand, within DvMF_0260 at 298.159 kb on + strand, within DvMF_0260 at 298.178 kb on - strand, within DvMF_0260 at 298.242 kb on + strand, within DvMF_0260 at 298.768 kb on + strand at 299.150 kb on - strand, within DvMF_0261 at 299.212 kb on + strand, within DvMF_0261 at 299.212 kb on + strand, within DvMF_0261 at 299.311 kb on + strand, within DvMF_0261 at 299.406 kb on + strand, within DvMF_0261 at 299.518 kb on - strand, within DvMF_0261
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1.4 mg/ml remove 297,092 - DvMF_0259 0.70 -3.2 297,118 + DvMF_0259 0.73 -4.0 297,441 + -1.4 297,485 - -1.7 297,832 + -1.0 297,837 + -1.4 297,840 - -0.8 297,965 + DvMF_0260 0.19 -1.7 298,159 + DvMF_0260 0.39 -1.6 298,159 + DvMF_0260 0.39 -0.6 298,178 - DvMF_0260 0.41 -0.3 298,242 + DvMF_0260 0.48 -1.8 298,768 + -0.3 299,150 - DvMF_0261 0.17 -0.6 299,212 + DvMF_0261 0.26 -1.1 299,212 + DvMF_0261 0.26 -0.1 299,311 + DvMF_0261 0.39 -0.4 299,406 + DvMF_0261 0.52 -0.6 299,518 - DvMF_0261 0.67 -0.8
Or see this region's nucleotide sequence