Experiment: MoYLS4 with Bacitracin 1.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0112 and DvMF_0113 are separated by 397 nucleotides DvMF_0113 and DvMF_0114 are separated by 22 nucleotides DvMF_0114 and DvMF_0115 are separated by 17 nucleotides
DvMF_0112: DvMF_0112 - ATP-dependent protease La (RefSeq), at 121,895 to 124,357
_0112
DvMF_0113: DvMF_0113 - hypothetical protein (RefSeq), at 124,755 to 125,330
_0113
DvMF_0114: DvMF_0114 - hypothetical protein (RefSeq), at 125,353 to 125,619
_0114
DvMF_0115: DvMF_0115 - hypothetical protein (RefSeq), at 125,637 to 126,578
_0115
Position (kb) 124
125
126 Strain fitness (log2 ratio) -2
-1
0
1 at 123.831 kb on - strand, within DvMF_0112 at 124.375 kb on - strand at 124.789 kb on + strand at 124.854 kb on + strand, within DvMF_0113 at 124.869 kb on - strand, within DvMF_0113 at 125.031 kb on + strand, within DvMF_0113 at 125.032 kb on + strand, within DvMF_0113 at 125.035 kb on - strand, within DvMF_0113 at 125.209 kb on - strand, within DvMF_0113 at 125.270 kb on - strand, within DvMF_0113 at 125.398 kb on + strand, within DvMF_0114 at 125.485 kb on - strand, within DvMF_0114 at 125.719 kb on - strand at 125.762 kb on + strand, within DvMF_0115 at 125.875 kb on + strand, within DvMF_0115 at 126.089 kb on - strand, within DvMF_0115
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1.4 mg/ml remove 123,831 - DvMF_0112 0.79 -0.9 124,375 - -0.0 124,789 + -0.6 124,854 + DvMF_0113 0.17 -1.6 124,869 - DvMF_0113 0.20 -0.3 125,031 + DvMF_0113 0.48 -0.2 125,032 + DvMF_0113 0.48 -0.4 125,035 - DvMF_0113 0.49 -0.5 125,209 - DvMF_0113 0.79 +0.8 125,270 - DvMF_0113 0.89 -0.5 125,398 + DvMF_0114 0.17 -0.6 125,485 - DvMF_0114 0.49 -1.8 125,719 - -1.3 125,762 + DvMF_0115 0.13 -2.0 125,875 + DvMF_0115 0.25 -1.2 126,089 - DvMF_0115 0.48 -0.4
Or see this region's nucleotide sequence