Experiment: MoYLS4 with Bacitracin 1.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0016 and DvMF_0017 are separated by 71 nucleotides DvMF_0017 and DvMF_0018 are separated by 3 nucleotides DvMF_0018 and DvMF_0019 are separated by 37 nucleotides
DvMF_0016: DvMF_0016 - transferase, putative (RefSeq), at 21,831 to 22,622
_0016
DvMF_0017: DvMF_0017 - Methyltransferase type 11 (RefSeq), at 22,694 to 23,458
_0017
DvMF_0018: DvMF_0018 - adenylylsulfate kinase (RefSeq), at 23,462 to 24,049
_0018
DvMF_0019: DvMF_0019 - hypothetical protein (RefSeq), at 24,087 to 25,421
_0019
Position (kb) 23
24
25 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 23.086 kb on + strand, within DvMF_0017 at 23.304 kb on + strand, within DvMF_0017 at 23.334 kb on + strand, within DvMF_0017 at 23.460 kb on - strand at 23.723 kb on - strand, within DvMF_0018 at 24.058 kb on + strand at 24.058 kb on + strand at 24.066 kb on - strand at 24.066 kb on - strand at 24.109 kb on - strand at 24.463 kb on + strand, within DvMF_0019 at 24.764 kb on + strand, within DvMF_0019 at 24.894 kb on + strand, within DvMF_0019 at 24.979 kb on + strand, within DvMF_0019 at 24.980 kb on - strand, within DvMF_0019 at 25.033 kb on - strand, within DvMF_0019 at 25.049 kb on + strand, within DvMF_0019
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Bacitracin 1.4 mg/ml remove 23,086 + DvMF_0017 0.51 +1.3 23,304 + DvMF_0017 0.80 -0.8 23,334 + DvMF_0017 0.84 -0.4 23,460 - -1.0 23,723 - DvMF_0018 0.44 +0.0 24,058 + -1.1 24,058 + +0.0 24,066 - -0.1 24,066 - -0.3 24,109 - -0.2 24,463 + DvMF_0019 0.28 +0.5 24,764 + DvMF_0019 0.51 -0.4 24,894 + DvMF_0019 0.60 -0.5 24,979 + DvMF_0019 0.67 -0.6 24,980 - DvMF_0019 0.67 +0.7 25,033 - DvMF_0019 0.71 -3.2 25,049 + DvMF_0019 0.72 -0.2
Or see this region's nucleotide sequence