Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3208 and ksgA are separated by 14 nucleotides ksgA and apaG are separated by 55 nucleotides apaG and apaH are separated by 21 nucleotides apaH and Shewana3_3212 are separated by 96 nucleotides
Shewana3_3208: Shewana3_3208 - 4-hydroxythreonine-4-phosphate dehydrogenase (RefSeq), at 3,852,649 to 3,853,635
_3208
Shewana3_3209: ksgA - dimethyladenosine transferase (RefSeq), at 3,853,650 to 3,854,456
ksgA
Shewana3_3210: apaG - ApaG (RefSeq), at 3,854,512 to 3,854,892
apaG
Shewana3_3211: apaH - diadenosine tetraphosphatase (RefSeq), at 3,854,914 to 3,855,738
apaH
Shewana3_3212: Shewana3_3212 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 3,855,835 to 3,857,856
_3212
Position (kb)
3854
3855 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3854.347 kb on + strand, within ksgA at 3854.495 kb on - strand at 3854.505 kb on - strand at 3854.571 kb on + strand, within apaG at 3854.825 kb on + strand, within apaG at 3854.994 kb on + strand at 3855.312 kb on - strand, within apaH at 3855.338 kb on + strand, within apaH at 3855.413 kb on + strand, within apaH at 3855.759 kb on + strand at 3855.759 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 3,854,347 + ksgA Shewana3_3209 0.86 +0.5 3,854,495 - +2.1 3,854,505 - +1.5 3,854,571 + apaG Shewana3_3210 0.15 -0.1 3,854,825 + apaG Shewana3_3210 0.82 -3.0 3,854,994 + -0.7 3,855,312 - apaH Shewana3_3211 0.48 -0.1 3,855,338 + apaH Shewana3_3211 0.51 -1.2 3,855,413 + apaH Shewana3_3211 0.60 -0.3 3,855,759 + -0.1 3,855,759 + +1.3
Or see this region's nucleotide sequence