Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and Shewana3_1610 are separated by 47 nucleotides Shewana3_1610 and Shewana3_R0079 are separated by 210 nucleotides Shewana3_R0079 and Shewana3_R0080 are separated by 20 nucleotides Shewana3_R0080 and Shewana3_R0081 are separated by 48 nucleotides Shewana3_R0081 and Shewana3_R0082 are separated by 131 nucleotides
Shewana3_1609: uvrC - excinuclease ABC subunit C (RefSeq), at 1,889,881 to 1,891,710
uvrC
Shewana3_1610: Shewana3_1610 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (RefSeq), at 1,891,758 to 1,892,306
_1610
Shewana3_R0079: Shewana3_R0079 - tRNA-Gly (RefSeq), at 1,892,517 to 1,892,592
_R0079
Shewana3_R0080: Shewana3_R0080 - tRNA-Cys (RefSeq), at 1,892,613 to 1,892,686
_R0080
Shewana3_R0081: Shewana3_R0081 - tRNA-Leu (RefSeq), at 1,892,735 to 1,892,821
_R0081
Shewana3_R0082: Shewana3_R0082 - tRNA-Leu (RefSeq), at 1,892,953 to 1,893,039
_R0082
Position (kb) 1891
1892
1893 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1891.120 kb on - strand, within uvrC at 1891.138 kb on - strand, within uvrC at 1891.379 kb on + strand, within uvrC at 1891.461 kb on - strand, within uvrC at 1891.578 kb on - strand at 1891.579 kb on - strand at 1891.589 kb on - strand at 1892.555 kb on + strand, within Shewana3_R0079 at 1892.569 kb on + strand, within Shewana3_R0079 at 1893.016 kb on + strand, within Shewana3_R0082 at 1893.282 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 1,891,120 - uvrC Shewana3_1609 0.68 +1.4 1,891,138 - uvrC Shewana3_1609 0.69 -0.9 1,891,379 + uvrC Shewana3_1609 0.82 -1.4 1,891,461 - uvrC Shewana3_1609 0.86 -0.6 1,891,578 - -1.8 1,891,579 - -0.2 1,891,589 - -1.4 1,892,555 + Shewana3_R0079 0.50 +2.5 1,892,569 + Shewana3_R0079 0.68 -0.1 1,893,016 + Shewana3_R0082 0.72 +0.7 1,893,282 + +0.4
Or see this region's nucleotide sequence