Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1225 and Shewana3_1226 are separated by 144 nucleotides Shewana3_1226 and Shewana3_1227 are separated by 32 nucleotides Shewana3_1227 and Shewana3_1228 overlap by 4 nucleotides
Shewana3_1225: Shewana3_1225 - hypothetical protein (RefSeq), at 1,428,213 to 1,429,304
_1225
Shewana3_1226: Shewana3_1226 - hypothetical protein (RefSeq), at 1,429,449 to 1,430,570
_1226
Shewana3_1227: Shewana3_1227 - type IV pilus biogenesis/stability protein PilW (RefSeq), at 1,430,603 to 1,431,391
_1227
Shewana3_1228: Shewana3_1228 - hypothetical protein (RefSeq), at 1,431,388 to 1,432,515
_1228
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1428.805 kb on + strand, within Shewana3_1225 at 1428.845 kb on - strand, within Shewana3_1225 at 1428.944 kb on + strand, within Shewana3_1225 at 1428.952 kb on - strand, within Shewana3_1225 at 1429.108 kb on - strand, within Shewana3_1225 at 1429.217 kb on + strand at 1429.225 kb on - strand at 1429.225 kb on - strand at 1429.227 kb on + strand at 1429.230 kb on - strand at 1429.273 kb on + strand at 1429.359 kb on + strand at 1429.373 kb on - strand at 1429.740 kb on - strand, within Shewana3_1226 at 1429.796 kb on + strand, within Shewana3_1226 at 1429.827 kb on + strand, within Shewana3_1226 at 1430.022 kb on - strand, within Shewana3_1226 at 1430.136 kb on - strand, within Shewana3_1226 at 1430.224 kb on - strand, within Shewana3_1226 at 1430.230 kb on - strand, within Shewana3_1226 at 1430.278 kb on + strand, within Shewana3_1226 at 1430.329 kb on + strand, within Shewana3_1226 at 1430.329 kb on + strand, within Shewana3_1226 at 1430.337 kb on - strand, within Shewana3_1226 at 1430.821 kb on - strand, within Shewana3_1227 at 1430.925 kb on - strand, within Shewana3_1227 at 1431.499 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 1,428,805 + Shewana3_1225 0.54 +0.5 1,428,845 - Shewana3_1225 0.58 +1.2 1,428,944 + Shewana3_1225 0.67 +0.3 1,428,952 - Shewana3_1225 0.68 -1.0 1,429,108 - Shewana3_1225 0.82 -0.6 1,429,217 + +2.0 1,429,225 - -0.8 1,429,225 - -0.4 1,429,227 + -1.8 1,429,230 - -1.5 1,429,273 + +1.6 1,429,359 + -0.6 1,429,373 - -0.7 1,429,740 - Shewana3_1226 0.26 +1.4 1,429,796 + Shewana3_1226 0.31 -4.0 1,429,827 + Shewana3_1226 0.34 -4.5 1,430,022 - Shewana3_1226 0.51 +0.4 1,430,136 - Shewana3_1226 0.61 -2.7 1,430,224 - Shewana3_1226 0.69 -0.2 1,430,230 - Shewana3_1226 0.70 -0.2 1,430,278 + Shewana3_1226 0.74 -4.4 1,430,329 + Shewana3_1226 0.78 -4.4 1,430,329 + Shewana3_1226 0.78 -2.8 1,430,337 - Shewana3_1226 0.79 -1.4 1,430,821 - Shewana3_1227 0.28 +0.4 1,430,925 - Shewana3_1227 0.41 +0.4 1,431,499 + -0.2
Or see this region's nucleotide sequence