Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0154 and Shewana3_0155 are separated by 1 nucleotides Shewana3_0155 and Shewana3_0156 overlap by 14 nucleotides Shewana3_0156 and Shewana3_0157 overlap by 20 nucleotides Shewana3_0157 and Shewana3_0158 overlap by 4 nucleotides
Shewana3_0154: Shewana3_0154 - general secretion pathway protein G (RefSeq), at 179,970 to 180,404
_0154
Shewana3_0155: Shewana3_0155 - general secretion pathway protein H (RefSeq), at 180,406 to 180,978
_0155
Shewana3_0156: Shewana3_0156 - general secretion pathway protein I (RefSeq), at 180,965 to 181,333
_0156
Shewana3_0157: Shewana3_0157 - general secretion pathway protein J (RefSeq), at 181,314 to 182,081
_0157
Shewana3_0158: Shewana3_0158 - general secretion pathway protein K (RefSeq), at 182,078 to 183,076
_0158
Position (kb) 181
182
183 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 180.314 kb on - strand, within Shewana3_0154 at 180.329 kb on + strand, within Shewana3_0154 at 180.405 kb on + strand at 180.413 kb on - strand at 180.683 kb on - strand, within Shewana3_0155 at 180.748 kb on + strand, within Shewana3_0155 at 180.792 kb on + strand, within Shewana3_0155 at 180.877 kb on - strand, within Shewana3_0155 at 180.917 kb on + strand, within Shewana3_0155 at 180.950 kb on + strand at 180.957 kb on - strand at 180.979 kb on + strand at 180.979 kb on + strand at 180.979 kb on + strand at 180.979 kb on + strand at 180.987 kb on - strand at 181.053 kb on + strand, within Shewana3_0156 at 181.199 kb on + strand, within Shewana3_0156 at 181.303 kb on + strand at 181.353 kb on - strand at 181.423 kb on - strand, within Shewana3_0157 at 181.423 kb on - strand, within Shewana3_0157 at 181.423 kb on - strand, within Shewana3_0157 at 181.425 kb on + strand, within Shewana3_0157 at 181.425 kb on + strand, within Shewana3_0157 at 181.541 kb on + strand, within Shewana3_0157 at 181.558 kb on + strand, within Shewana3_0157 at 181.568 kb on + strand, within Shewana3_0157 at 181.576 kb on - strand, within Shewana3_0157 at 181.671 kb on - strand, within Shewana3_0157 at 181.855 kb on - strand, within Shewana3_0157 at 181.856 kb on - strand, within Shewana3_0157 at 181.979 kb on + strand, within Shewana3_0157 at 182.082 kb on - strand at 182.202 kb on + strand, within Shewana3_0158 at 182.208 kb on + strand, within Shewana3_0158 at 182.290 kb on - strand, within Shewana3_0158 at 182.300 kb on - strand, within Shewana3_0158 at 182.484 kb on - strand, within Shewana3_0158 at 182.752 kb on + strand, within Shewana3_0158 at 182.753 kb on - strand, within Shewana3_0158 at 182.869 kb on + strand, within Shewana3_0158 at 182.919 kb on - strand, within Shewana3_0158
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 180,314 - Shewana3_0154 0.79 -2.4 180,329 + Shewana3_0154 0.83 -2.9 180,405 + +0.3 180,413 - -2.8 180,683 - Shewana3_0155 0.48 -2.9 180,748 + Shewana3_0155 0.60 -3.2 180,792 + Shewana3_0155 0.67 -3.4 180,877 - Shewana3_0155 0.82 -0.9 180,917 + Shewana3_0155 0.89 +0.7 180,950 + +0.4 180,957 - -1.6 180,979 + -2.4 180,979 + +0.4 180,979 + -1.5 180,979 + -0.4 180,987 - -2.1 181,053 + Shewana3_0156 0.24 -2.3 181,199 + Shewana3_0156 0.63 -0.9 181,303 + +0.1 181,353 - -1.4 181,423 - Shewana3_0157 0.14 -2.8 181,423 - Shewana3_0157 0.14 -0.7 181,423 - Shewana3_0157 0.14 -2.4 181,425 + Shewana3_0157 0.14 -4.4 181,425 + Shewana3_0157 0.14 -3.8 181,541 + Shewana3_0157 0.30 -1.1 181,558 + Shewana3_0157 0.32 -3.0 181,568 + Shewana3_0157 0.33 -0.7 181,576 - Shewana3_0157 0.34 -0.1 181,671 - Shewana3_0157 0.46 -4.3 181,855 - Shewana3_0157 0.70 -4.2 181,856 - Shewana3_0157 0.71 -1.5 181,979 + Shewana3_0157 0.87 -1.0 182,082 - -3.2 182,202 + Shewana3_0158 0.12 +0.3 182,208 + Shewana3_0158 0.13 -2.1 182,290 - Shewana3_0158 0.21 -1.3 182,300 - Shewana3_0158 0.22 +2.1 182,484 - Shewana3_0158 0.41 -1.8 182,752 + Shewana3_0158 0.67 -1.0 182,753 - Shewana3_0158 0.68 -3.5 182,869 + Shewana3_0158 0.79 -4.4 182,919 - Shewana3_0158 0.84 -2.2
Or see this region's nucleotide sequence