Experiment: Chloride 500 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0115 and Shewana3_0116 are separated by 197 nucleotides Shewana3_0116 and Shewana3_0117 are separated by 233 nucleotides Shewana3_0117 and Shewana3_0118 are separated by 1 nucleotides Shewana3_0118 and Shewana3_0119 are separated by 25 nucleotides
Shewana3_0115: Shewana3_0115 - hypothetical protein (RefSeq), at 131,341 to 131,982
_0115
Shewana3_0116: Shewana3_0116 - hypothetical protein (RefSeq), at 132,180 to 132,563
_0116
Shewana3_0117: Shewana3_0117 - MscS mechanosensitive ion channel (RefSeq), at 132,797 to 134,065
_0117
Shewana3_0118: Shewana3_0118 - lysine exporter protein LysE/YggA (RefSeq), at 134,067 to 134,690
_0118
Shewana3_0119: Shewana3_0119 - phospholipid/glycerol acyltransferase (RefSeq), at 134,716 to 135,276
_0119
Position (kb) 132
133
134
135 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 131.870 kb on + strand, within Shewana3_0115 at 131.878 kb on - strand, within Shewana3_0115 at 131.889 kb on + strand, within Shewana3_0115 at 131.897 kb on - strand, within Shewana3_0115 at 131.903 kb on - strand, within Shewana3_0115 at 131.951 kb on - strand at 132.100 kb on + strand at 132.163 kb on + strand at 132.176 kb on + strand at 132.183 kb on - strand at 132.222 kb on + strand, within Shewana3_0116 at 132.247 kb on - strand, within Shewana3_0116 at 132.297 kb on - strand, within Shewana3_0116 at 132.299 kb on + strand, within Shewana3_0116 at 132.334 kb on + strand, within Shewana3_0116 at 132.334 kb on + strand, within Shewana3_0116 at 132.342 kb on - strand, within Shewana3_0116 at 132.368 kb on + strand, within Shewana3_0116 at 132.383 kb on + strand, within Shewana3_0116 at 132.518 kb on + strand, within Shewana3_0116 at 132.526 kb on - strand at 132.526 kb on - strand at 132.558 kb on - strand at 132.637 kb on - strand at 132.697 kb on + strand at 132.737 kb on - strand at 132.752 kb on + strand at 132.890 kb on - strand at 133.008 kb on + strand, within Shewana3_0117 at 133.090 kb on - strand, within Shewana3_0117 at 133.094 kb on + strand, within Shewana3_0117 at 133.104 kb on + strand, within Shewana3_0117 at 133.104 kb on - strand, within Shewana3_0117 at 133.112 kb on - strand, within Shewana3_0117 at 133.326 kb on + strand, within Shewana3_0117 at 133.334 kb on - strand, within Shewana3_0117 at 133.470 kb on - strand, within Shewana3_0117 at 133.734 kb on - strand, within Shewana3_0117 at 134.087 kb on - strand at 134.097 kb on + strand at 134.193 kb on - strand, within Shewana3_0118 at 134.216 kb on + strand, within Shewana3_0118 at 134.307 kb on + strand, within Shewana3_0118 at 134.307 kb on + strand, within Shewana3_0118 at 134.315 kb on - strand, within Shewana3_0118 at 134.316 kb on - strand, within Shewana3_0118 at 134.564 kb on - strand, within Shewana3_0118 at 134.564 kb on - strand, within Shewana3_0118 at 134.564 kb on - strand, within Shewana3_0118 at 134.569 kb on - strand, within Shewana3_0118 at 134.766 kb on + strand at 134.774 kb on - strand, within Shewana3_0119 at 134.774 kb on - strand, within Shewana3_0119
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 500 mM remove 131,870 + Shewana3_0115 0.82 -0.3 131,878 - Shewana3_0115 0.84 +1.1 131,889 + Shewana3_0115 0.85 -1.4 131,897 - Shewana3_0115 0.87 -0.3 131,903 - Shewana3_0115 0.88 -0.2 131,951 - +0.3 132,100 + +1.7 132,163 + -0.1 132,176 + +0.7 132,183 - +1.6 132,222 + Shewana3_0116 0.11 -2.6 132,247 - Shewana3_0116 0.17 +0.5 132,297 - Shewana3_0116 0.30 -1.0 132,299 + Shewana3_0116 0.31 -0.5 132,334 + Shewana3_0116 0.40 -0.1 132,334 + Shewana3_0116 0.40 -1.7 132,342 - Shewana3_0116 0.42 -1.0 132,368 + Shewana3_0116 0.49 +0.7 132,383 + Shewana3_0116 0.53 +0.7 132,518 + Shewana3_0116 0.88 -0.6 132,526 - -0.3 132,526 - +1.0 132,558 - +0.4 132,637 - -0.9 132,697 + -0.0 132,737 - -0.2 132,752 + -0.2 132,890 - +0.6 133,008 + Shewana3_0117 0.17 -0.2 133,090 - Shewana3_0117 0.23 -0.5 133,094 + Shewana3_0117 0.23 -1.0 133,104 + Shewana3_0117 0.24 -1.2 133,104 - Shewana3_0117 0.24 -0.4 133,112 - Shewana3_0117 0.25 -0.1 133,326 + Shewana3_0117 0.42 -0.3 133,334 - Shewana3_0117 0.42 -3.3 133,470 - Shewana3_0117 0.53 -0.7 133,734 - Shewana3_0117 0.74 +0.5 134,087 - -0.8 134,097 + -0.1 134,193 - Shewana3_0118 0.20 -0.7 134,216 + Shewana3_0118 0.24 -0.4 134,307 + Shewana3_0118 0.38 -2.3 134,307 + Shewana3_0118 0.38 +1.1 134,315 - Shewana3_0118 0.40 -0.4 134,316 - Shewana3_0118 0.40 +0.9 134,564 - Shewana3_0118 0.80 -1.4 134,564 - Shewana3_0118 0.80 -0.4 134,564 - Shewana3_0118 0.80 +1.7 134,569 - Shewana3_0118 0.80 +0.3 134,766 + -0.3 134,774 - Shewana3_0119 0.10 -0.6 134,774 - Shewana3_0119 0.10 -0.4
Or see this region's nucleotide sequence