Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25280 and AO353_25285 are separated by 151 nucleotides AO353_25285 and AO353_25290 overlap by 4 nucleotides AO353_25290 and AO353_25295 are separated by 3 nucleotides
AO353_25280: AO353_25280 - gamma-glutamyltransferase, at 5,576,496 to 5,578,106
_25280
AO353_25285: AO353_25285 - transglutaminase, at 5,578,258 to 5,579,055
_25285
AO353_25290: AO353_25290 - hypothetical protein, at 5,579,052 to 5,580,002
_25290
AO353_25295: AO353_25295 - hypothetical protein, at 5,580,006 to 5,581,415
_25295
Position (kb) 5578
5579
5580 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5577.331 kb on + strand, within AO353_25280 at 5577.331 kb on + strand, within AO353_25280 at 5577.384 kb on + strand, within AO353_25280 at 5577.385 kb on - strand, within AO353_25280 at 5577.385 kb on - strand, within AO353_25280 at 5577.517 kb on + strand, within AO353_25280 at 5577.518 kb on - strand, within AO353_25280 at 5577.518 kb on - strand, within AO353_25280 at 5577.518 kb on - strand, within AO353_25280 at 5577.576 kb on - strand, within AO353_25280 at 5578.076 kb on - strand at 5578.081 kb on + strand at 5578.082 kb on - strand at 5578.123 kb on + strand at 5578.228 kb on - strand at 5578.228 kb on - strand at 5578.259 kb on + strand at 5578.260 kb on - strand at 5578.260 kb on - strand at 5578.362 kb on - strand, within AO353_25285 at 5578.430 kb on + strand, within AO353_25285 at 5578.514 kb on + strand, within AO353_25285 at 5578.514 kb on + strand, within AO353_25285 at 5578.564 kb on - strand, within AO353_25285 at 5578.564 kb on - strand, within AO353_25285 at 5578.626 kb on + strand, within AO353_25285 at 5578.641 kb on - strand, within AO353_25285 at 5578.641 kb on - strand, within AO353_25285 at 5578.832 kb on + strand, within AO353_25285 at 5578.832 kb on + strand, within AO353_25285 at 5578.833 kb on - strand, within AO353_25285 at 5578.876 kb on + strand, within AO353_25285 at 5578.876 kb on + strand, within AO353_25285 at 5578.876 kb on + strand, within AO353_25285 at 5578.876 kb on + strand, within AO353_25285 at 5578.876 kb on + strand, within AO353_25285 at 5578.876 kb on + strand, within AO353_25285 at 5578.876 kb on + strand, within AO353_25285 at 5578.876 kb on + strand, within AO353_25285 at 5578.877 kb on - strand, within AO353_25285 at 5578.916 kb on - strand, within AO353_25285 at 5578.916 kb on - strand, within AO353_25285 at 5579.019 kb on - strand at 5579.024 kb on + strand at 5579.024 kb on + strand at 5579.024 kb on + strand at 5579.024 kb on + strand at 5579.025 kb on - strand at 5579.025 kb on - strand at 5579.056 kb on - strand at 5579.068 kb on + strand at 5579.398 kb on + strand, within AO353_25290 at 5579.401 kb on + strand, within AO353_25290 at 5579.446 kb on + strand, within AO353_25290 at 5579.525 kb on - strand, within AO353_25290 at 5579.615 kb on - strand, within AO353_25290 at 5579.743 kb on + strand, within AO353_25290 at 5579.743 kb on + strand, within AO353_25290 at 5579.743 kb on + strand, within AO353_25290 at 5579.743 kb on + strand, within AO353_25290 at 5579.743 kb on + strand, within AO353_25290 at 5579.743 kb on + strand, within AO353_25290 at 5579.743 kb on + strand, within AO353_25290 at 5579.743 kb on + strand, within AO353_25290 at 5579.744 kb on - strand, within AO353_25290 at 5579.744 kb on - strand, within AO353_25290 at 5579.744 kb on - strand, within AO353_25290 at 5579.744 kb on - strand, within AO353_25290 at 5579.744 kb on - strand, within AO353_25290 at 5579.744 kb on - strand, within AO353_25290 at 5579.747 kb on + strand, within AO353_25290 at 5579.747 kb on + strand, within AO353_25290 at 5579.748 kb on - strand, within AO353_25290 at 5579.748 kb on - strand, within AO353_25290 at 5579.824 kb on + strand, within AO353_25290 at 5579.825 kb on - strand, within AO353_25290 at 5579.825 kb on - strand, within AO353_25290 at 5579.915 kb on - strand at 5579.975 kb on - strand at 5580.007 kb on + strand at 5580.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,577,331 + AO353_25280 0.52 -0.6 5,577,331 + AO353_25280 0.52 +0.0 5,577,384 + AO353_25280 0.55 -0.1 5,577,385 - AO353_25280 0.55 +0.4 5,577,385 - AO353_25280 0.55 -1.4 5,577,517 + AO353_25280 0.63 +0.1 5,577,518 - AO353_25280 0.63 +2.1 5,577,518 - AO353_25280 0.63 +0.5 5,577,518 - AO353_25280 0.63 +0.6 5,577,576 - AO353_25280 0.67 -1.1 5,578,076 - -1.2 5,578,081 + -1.4 5,578,082 - -0.2 5,578,123 + +0.5 5,578,228 - -0.9 5,578,228 - -0.5 5,578,259 + -2.1 5,578,260 - +1.4 5,578,260 - +0.7 5,578,362 - AO353_25285 0.13 +1.5 5,578,430 + AO353_25285 0.22 +1.1 5,578,514 + AO353_25285 0.32 +1.9 5,578,514 + AO353_25285 0.32 +0.4 5,578,564 - AO353_25285 0.38 -2.1 5,578,564 - AO353_25285 0.38 +1.6 5,578,626 + AO353_25285 0.46 +0.8 5,578,641 - AO353_25285 0.48 -0.2 5,578,641 - AO353_25285 0.48 +0.1 5,578,832 + AO353_25285 0.72 -0.4 5,578,832 + AO353_25285 0.72 +0.3 5,578,833 - AO353_25285 0.72 -0.5 5,578,876 + AO353_25285 0.77 -0.8 5,578,876 + AO353_25285 0.77 -1.5 5,578,876 + AO353_25285 0.77 +0.4 5,578,876 + AO353_25285 0.77 -0.4 5,578,876 + AO353_25285 0.77 -0.5 5,578,876 + AO353_25285 0.77 +0.0 5,578,876 + AO353_25285 0.77 -1.2 5,578,876 + AO353_25285 0.77 +0.9 5,578,877 - AO353_25285 0.78 -0.5 5,578,916 - AO353_25285 0.82 +1.1 5,578,916 - AO353_25285 0.82 -1.6 5,579,019 - -0.7 5,579,024 + -0.8 5,579,024 + -0.7 5,579,024 + +0.2 5,579,024 + +0.4 5,579,025 - +0.5 5,579,025 - -0.7 5,579,056 - -1.1 5,579,068 + +0.1 5,579,398 + AO353_25290 0.36 -0.3 5,579,401 + AO353_25290 0.37 +3.6 5,579,446 + AO353_25290 0.41 +0.5 5,579,525 - AO353_25290 0.50 +0.8 5,579,615 - AO353_25290 0.59 +1.3 5,579,743 + AO353_25290 0.73 -0.5 5,579,743 + AO353_25290 0.73 -0.9 5,579,743 + AO353_25290 0.73 +1.0 5,579,743 + AO353_25290 0.73 -2.1 5,579,743 + AO353_25290 0.73 -0.7 5,579,743 + AO353_25290 0.73 +0.2 5,579,743 + AO353_25290 0.73 +1.5 5,579,743 + AO353_25290 0.73 -1.4 5,579,744 - AO353_25290 0.73 +1.1 5,579,744 - AO353_25290 0.73 -0.4 5,579,744 - AO353_25290 0.73 +0.5 5,579,744 - AO353_25290 0.73 +0.4 5,579,744 - AO353_25290 0.73 +0.4 5,579,744 - AO353_25290 0.73 +0.5 5,579,747 + AO353_25290 0.73 -0.5 5,579,747 + AO353_25290 0.73 -1.0 5,579,748 - AO353_25290 0.73 +0.0 5,579,748 - AO353_25290 0.73 +1.5 5,579,824 + AO353_25290 0.81 -0.9 5,579,825 - AO353_25290 0.81 +0.9 5,579,825 - AO353_25290 0.81 -0.5 5,579,915 - +0.4 5,579,975 - -2.9 5,580,007 + +1.8 5,580,008 - -1.6
Or see this region's nucleotide sequence