Experiment: Sisomicin 0.008 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt AO353_25155 and AO353_25160 are separated by 33 nucleotides AO353_25160 and AO353_25165 overlap by 23 nucleotides AO353_25165 and AO353_25170 are separated by 343 nucleotides 
        AO353_25155: AO353_25155 - pyruvate dehydrogenase, at 5,549,269 to 5,550,288 
        _25155 
         
        
        AO353_25160: AO353_25160 - ABC transporter substrate-binding protein, at 5,550,322 to 5,551,299 
        _25160 
         
        
        AO353_25165: AO353_25165 - ATP-NAD kinase, at 5,551,277 to 5,552,377 
        _25165 
         
        
        AO353_25170: AO353_25170 - Fis family transcriptional regulator, at 5,552,721 to 5,554,613 
        _25170 
         Position (kb) 5551 
5552 
5553 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 
3 at 5550.310 kb on + strand at 5550.311 kb on - strand at 5550.312 kb on + strand at 5550.312 kb on + strand at 5550.313 kb on - strand at 5550.313 kb on - strand at 5550.338 kb on + strand at 5550.339 kb on - strand at 5550.339 kb on - strand at 5550.573 kb on - strand, within AO353_25160 at 5550.576 kb on - strand, within AO353_25160 at 5551.037 kb on + strand, within AO353_25160 at 5551.037 kb on + strand, within AO353_25160 at 5551.038 kb on - strand, within AO353_25160 at 5551.060 kb on + strand, within AO353_25160 at 5551.060 kb on + strand, within AO353_25160 at 5551.060 kb on + strand, within AO353_25160 at 5551.061 kb on - strand, within AO353_25160 at 5551.172 kb on + strand, within AO353_25160 at 5551.172 kb on + strand, within AO353_25160 at 5551.173 kb on - strand, within AO353_25160 at 5551.173 kb on - strand, within AO353_25160 at 5551.303 kb on + strand at 5551.389 kb on + strand, within AO353_25165 at 5551.390 kb on - strand, within AO353_25165 at 5551.390 kb on - strand, within AO353_25165 at 5551.390 kb on - strand, within AO353_25165 at 5551.390 kb on - strand, within AO353_25165 at 5551.522 kb on + strand, within AO353_25165 at 5551.522 kb on + strand, within AO353_25165 at 5551.523 kb on - strand, within AO353_25165 at 5551.853 kb on - strand, within AO353_25165 at 5551.934 kb on + strand, within AO353_25165 at 5551.934 kb on + strand, within AO353_25165 at 5551.935 kb on - strand, within AO353_25165 at 5551.935 kb on - strand, within AO353_25165 at 5551.935 kb on - strand, within AO353_25165 at 5552.350 kb on + strand at 5552.350 kb on + strand at 5552.350 kb on + strand at 5552.350 kb on + strand at 5552.350 kb on + strand at 5552.350 kb on + strand at 5552.351 kb on - strand at 5552.351 kb on - strand at 5552.351 kb on - strand at 5552.354 kb on + strand at 5552.354 kb on + strand at 5552.354 kb on + strand at 5552.355 kb on - strand at 5552.355 kb on - strand at 5552.355 kb on - strand at 5552.436 kb on + strand at 5552.437 kb on - strand at 5552.437 kb on - strand at 5552.473 kb on - strand at 5552.679 kb on + strand at 5552.680 kb on - strand at 5552.695 kb on - strand at 5552.869 kb on + strand at 5552.870 kb on - strand at 5553.081 kb on + strand, within AO353_25170 at 5553.081 kb on + strand, within AO353_25170 at 5553.082 kb on - strand, within AO353_25170 at 5553.082 kb on - strand, within AO353_25170 at 5553.082 kb on - strand, within AO353_25170 at 5553.082 kb on - strand, within AO353_25170 at 5553.116 kb on + strand, within AO353_25170 at 5553.117 kb on - strand, within AO353_25170 at 5553.117 kb on - strand, within AO353_25170 at 5553.117 kb on - strand, within AO353_25170 at 5553.117 kb on - strand, within AO353_25170 at 5553.117 kb on - strand, within AO353_25170 at 5553.117 kb on - strand, within AO353_25170 at 5553.375 kb on + strand, within AO353_25170  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Sisomicin 0.008 mg/ml remove 5,550,310 +  +0.7 5,550,311 -  -0.9 5,550,312 +  +2.1 5,550,312 +  +1.3 5,550,313 -  +1.1 5,550,313 -  -0.1 5,550,338 +  -0.8 5,550,339 -  -0.7 5,550,339 -  -1.6 5,550,573 -  AO353_25160 0.26  +0.1 5,550,576 -  AO353_25160 0.26  +0.4 5,551,037 +  AO353_25160 0.73  +0.6 5,551,037 +  AO353_25160 0.73  -0.2 5,551,038 -  AO353_25160 0.73  -0.9 5,551,060 +  AO353_25160 0.75  +0.3 5,551,060 +  AO353_25160 0.75  -0.7 5,551,060 +  AO353_25160 0.75  -1.6 5,551,061 -  AO353_25160 0.76  +0.9 5,551,172 +  AO353_25160 0.87  -1.2 5,551,172 +  AO353_25160 0.87  +1.1 5,551,173 -  AO353_25160 0.87  +0.3 5,551,173 -  AO353_25160 0.87  +1.4 5,551,303 +  +0.1 5,551,389 +  AO353_25165 0.10  -0.7 5,551,390 -  AO353_25165 0.10  -1.6 5,551,390 -  AO353_25165 0.10  +1.1 5,551,390 -  AO353_25165 0.10  -0.9 5,551,390 -  AO353_25165 0.10  -0.7 5,551,522 +  AO353_25165 0.22  -0.1 5,551,522 +  AO353_25165 0.22  -1.9 5,551,523 -  AO353_25165 0.22  -0.1 5,551,853 -  AO353_25165 0.52  -0.2 5,551,934 +  AO353_25165 0.60  -0.9 5,551,934 +  AO353_25165 0.60  -1.8 5,551,935 -  AO353_25165 0.60  -0.7 5,551,935 -  AO353_25165 0.60  -1.1 5,551,935 -  AO353_25165 0.60  -0.8 5,552,350 +  -0.7 5,552,350 +  -1.1 5,552,350 +  -0.7 5,552,350 +  -1.2 5,552,350 +  -0.7 5,552,350 +  -1.1 5,552,351 -  -2.0 5,552,351 -  -0.7 5,552,351 -  -0.5 5,552,354 +  -0.2 5,552,354 +  -0.1 5,552,354 +  -0.9 5,552,355 -  +1.1 5,552,355 -  +0.9 5,552,355 -  +0.5 5,552,436 +  -2.6 5,552,437 -  -0.7 5,552,437 -  -1.7 5,552,473 -  +0.2 5,552,679 +  -0.2 5,552,680 -  +3.1 5,552,695 -  -0.7 5,552,869 +  -1.9 5,552,870 -  +1.3 5,553,081 +  AO353_25170 0.19  -1.2 5,553,081 +  AO353_25170 0.19  -1.2 5,553,082 -  AO353_25170 0.19  -0.2 5,553,082 -  AO353_25170 0.19  -0.1 5,553,082 -  AO353_25170 0.19  -1.1 5,553,082 -  AO353_25170 0.19  +0.5 5,553,116 +  AO353_25170 0.21  +0.3 5,553,117 -  AO353_25170 0.21  +0.1 5,553,117 -  AO353_25170 0.21  +0.1 5,553,117 -  AO353_25170 0.21  +0.4 5,553,117 -  AO353_25170 0.21  -0.2 5,553,117 -  AO353_25170 0.21  -0.5 5,553,117 -  AO353_25170 0.21  -0.9 5,553,375 +  AO353_25170 0.35  +0.1 
Or see this region's nucleotide sequence