Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23605 and AO353_23610 overlap by 1 nucleotides AO353_23610 and AO353_23615 overlap by 4 nucleotides AO353_23615 and AO353_23620 are separated by 44 nucleotides AO353_23620 and AO353_23625 overlap by 4 nucleotides
AO353_23605: AO353_23605 - NAD kinase, at 5,168,184 to 5,169,074
_23605
AO353_23610: AO353_23610 - serine/threonine protein phosphatase, at 5,169,074 to 5,170,045
_23610
AO353_23615: AO353_23615 - peptidase S54, at 5,170,042 to 5,170,917
_23615
AO353_23620: AO353_23620 - hypothetical protein, at 5,170,962 to 5,171,222
_23620
AO353_23625: AO353_23625 - hypothetical protein, at 5,171,219 to 5,172,049
_23625
Position (kb)
5170
5171 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5169.091 kb on + strand at 5169.092 kb on - strand at 5169.092 kb on - strand at 5169.095 kb on + strand at 5169.095 kb on + strand at 5169.095 kb on + strand at 5169.095 kb on + strand at 5169.368 kb on + strand, within AO353_23610 at 5169.368 kb on + strand, within AO353_23610 at 5169.368 kb on + strand, within AO353_23610 at 5169.368 kb on + strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.470 kb on + strand, within AO353_23610 at 5169.470 kb on + strand, within AO353_23610 at 5169.471 kb on - strand, within AO353_23610 at 5169.560 kb on + strand, within AO353_23610 at 5169.560 kb on + strand, within AO353_23610 at 5169.561 kb on - strand, within AO353_23610 at 5169.561 kb on - strand, within AO353_23610 at 5169.561 kb on - strand, within AO353_23610 at 5169.752 kb on + strand at 5169.752 kb on + strand, within AO353_23610 at 5169.752 kb on + strand, within AO353_23610 at 5169.753 kb on - strand, within AO353_23610 at 5169.753 kb on - strand at 5169.829 kb on + strand, within AO353_23610 at 5169.829 kb on + strand, within AO353_23610 at 5169.843 kb on - strand, within AO353_23610 at 5169.878 kb on + strand, within AO353_23610 at 5169.878 kb on + strand, within AO353_23610 at 5169.932 kb on + strand, within AO353_23610 at 5169.932 kb on + strand, within AO353_23610 at 5169.932 kb on + strand, within AO353_23610 at 5169.947 kb on + strand, within AO353_23610 at 5170.061 kb on + strand at 5170.061 kb on + strand at 5170.061 kb on + strand at 5170.062 kb on - strand at 5170.062 kb on - strand at 5170.201 kb on + strand, within AO353_23615 at 5170.420 kb on + strand, within AO353_23615 at 5170.420 kb on + strand, within AO353_23615 at 5170.421 kb on - strand, within AO353_23615 at 5170.621 kb on + strand, within AO353_23615 at 5170.622 kb on - strand, within AO353_23615 at 5170.622 kb on - strand at 5170.630 kb on + strand, within AO353_23615 at 5170.631 kb on - strand, within AO353_23615 at 5170.633 kb on + strand, within AO353_23615 at 5170.636 kb on + strand, within AO353_23615 at 5170.706 kb on - strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.888 kb on + strand at 5170.888 kb on + strand at 5170.889 kb on - strand at 5170.889 kb on - strand at 5170.889 kb on - strand at 5170.889 kb on - strand at 5170.889 kb on - strand at 5170.927 kb on + strand at 5171.007 kb on + strand, within AO353_23620 at 5171.008 kb on - strand, within AO353_23620 at 5171.008 kb on - strand, within AO353_23620 at 5171.181 kb on - strand, within AO353_23620 at 5171.181 kb on - strand, within AO353_23620 at 5171.192 kb on + strand at 5171.192 kb on + strand, within AO353_23620 at 5171.286 kb on - strand at 5171.286 kb on - strand at 5171.354 kb on + strand, within AO353_23625 at 5171.354 kb on + strand, within AO353_23625 at 5171.355 kb on - strand, within AO353_23625 at 5171.417 kb on + strand, within AO353_23625 at 5171.487 kb on - strand, within AO353_23625 at 5171.487 kb on - strand, within AO353_23625 at 5171.552 kb on + strand, within AO353_23625 at 5171.552 kb on + strand, within AO353_23625 at 5171.553 kb on - strand, within AO353_23625 at 5171.553 kb on - strand, within AO353_23625 at 5171.553 kb on - strand, within AO353_23625 at 5171.596 kb on + strand, within AO353_23625 at 5171.596 kb on + strand, within AO353_23625 at 5171.856 kb on + strand, within AO353_23625 at 5171.856 kb on + strand, within AO353_23625
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,169,091 + -0.4 5,169,092 - -0.2 5,169,092 - -0.2 5,169,095 + -0.3 5,169,095 + +0.8 5,169,095 + -2.9 5,169,095 + +1.3 5,169,368 + AO353_23610 0.30 -0.4 5,169,368 + AO353_23610 0.30 -1.0 5,169,368 + AO353_23610 0.30 +0.0 5,169,368 + AO353_23610 0.30 +0.7 5,169,369 - AO353_23610 0.30 -0.2 5,169,369 - AO353_23610 0.30 -0.6 5,169,369 - AO353_23610 0.30 -1.2 5,169,369 - AO353_23610 0.30 +0.2 5,169,369 - AO353_23610 0.30 -0.6 5,169,470 + AO353_23610 0.41 +0.5 5,169,470 + AO353_23610 0.41 +1.3 5,169,471 - AO353_23610 0.41 -0.8 5,169,560 + AO353_23610 0.50 +0.2 5,169,560 + AO353_23610 0.50 +0.8 5,169,561 - AO353_23610 0.50 -0.8 5,169,561 - AO353_23610 0.50 -0.2 5,169,561 - AO353_23610 0.50 +0.0 5,169,752 + -0.6 5,169,752 + AO353_23610 0.70 -0.8 5,169,752 + AO353_23610 0.70 -2.2 5,169,753 - AO353_23610 0.70 -0.2 5,169,753 - -0.2 5,169,829 + AO353_23610 0.78 -0.7 5,169,829 + AO353_23610 0.78 +0.0 5,169,843 - AO353_23610 0.79 -1.3 5,169,878 + AO353_23610 0.83 -0.4 5,169,878 + AO353_23610 0.83 -0.5 5,169,932 + AO353_23610 0.88 -1.3 5,169,932 + AO353_23610 0.88 +0.7 5,169,932 + AO353_23610 0.88 -0.8 5,169,947 + AO353_23610 0.90 +1.2 5,170,061 + -1.2 5,170,061 + +0.2 5,170,061 + -0.2 5,170,062 - -0.6 5,170,062 - -0.8 5,170,201 + AO353_23615 0.18 -2.2 5,170,420 + AO353_23615 0.43 -0.8 5,170,420 + AO353_23615 0.43 +0.2 5,170,421 - AO353_23615 0.43 -0.4 5,170,621 + AO353_23615 0.66 +0.2 5,170,622 - AO353_23615 0.66 -2.0 5,170,622 - +0.2 5,170,630 + AO353_23615 0.67 -1.7 5,170,631 - AO353_23615 0.67 +0.6 5,170,633 + AO353_23615 0.67 +0.2 5,170,636 + AO353_23615 0.68 -2.0 5,170,706 - AO353_23615 0.76 -3.8 5,170,785 + AO353_23615 0.85 -1.2 5,170,785 + AO353_23615 0.85 -2.8 5,170,785 + AO353_23615 0.85 -2.7 5,170,785 + AO353_23615 0.85 -1.2 5,170,785 + AO353_23615 0.85 -1.0 5,170,786 - AO353_23615 0.85 -1.2 5,170,786 - AO353_23615 0.85 -1.7 5,170,786 - AO353_23615 0.85 -0.6 5,170,786 - AO353_23615 0.85 -2.2 5,170,786 - AO353_23615 0.85 -1.6 5,170,786 - AO353_23615 0.85 -0.2 5,170,786 - AO353_23615 0.85 -0.4 5,170,888 + -1.5 5,170,888 + -1.5 5,170,889 - -1.7 5,170,889 - -1.5 5,170,889 - -1.0 5,170,889 - -1.8 5,170,889 - +3.7 5,170,927 + +1.9 5,171,007 + AO353_23620 0.17 +1.5 5,171,008 - AO353_23620 0.18 +1.9 5,171,008 - AO353_23620 0.18 +1.0 5,171,181 - AO353_23620 0.84 -0.2 5,171,181 - AO353_23620 0.84 +0.6 5,171,192 + +0.2 5,171,192 + AO353_23620 0.88 -1.2 5,171,286 - -0.3 5,171,286 - +1.2 5,171,354 + AO353_23625 0.16 -1.0 5,171,354 + AO353_23625 0.16 -1.0 5,171,355 - AO353_23625 0.16 +0.6 5,171,417 + AO353_23625 0.24 -0.3 5,171,487 - AO353_23625 0.32 +0.6 5,171,487 - AO353_23625 0.32 +0.2 5,171,552 + AO353_23625 0.40 -2.5 5,171,552 + AO353_23625 0.40 +1.6 5,171,553 - AO353_23625 0.40 +0.3 5,171,553 - AO353_23625 0.40 +0.6 5,171,553 - AO353_23625 0.40 -0.5 5,171,596 + AO353_23625 0.45 +0.8 5,171,596 + AO353_23625 0.45 +0.7 5,171,856 + AO353_23625 0.77 -0.4 5,171,856 + AO353_23625 0.77 -1.3
Or see this region's nucleotide sequence