Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_22955 and AO353_22960 are separated by 155 nucleotides AO353_22960 and AO353_22965 are separated by 0 nucleotides AO353_22965 and AO353_22970 are separated by 84 nucleotides AO353_22970 and AO353_22975 are separated by 91 nucleotides
AO353_22955: AO353_22955 - tRNA hydroxylase, at 5,042,366 to 5,042,977
_22955
AO353_22960: AO353_22960 - transcriptional regulator, at 5,043,133 to 5,043,840
_22960
AO353_22965: AO353_22965 - ATPase, at 5,043,841 to 5,045,118
_22965
AO353_22970: AO353_22970 - structural protein MipA, at 5,045,203 to 5,046,006
_22970
AO353_22975: AO353_22975 - alanine acetyltransferase, at 5,046,098 to 5,046,586
_22975
Position (kb) 5043
5044
5045
5046 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 5042.849 kb on + strand, within AO353_22955 at 5042.849 kb on + strand, within AO353_22955 at 5043.007 kb on + strand at 5043.007 kb on + strand at 5043.007 kb on + strand at 5043.008 kb on - strand at 5043.091 kb on + strand at 5043.091 kb on + strand at 5043.091 kb on + strand at 5043.092 kb on - strand at 5043.092 kb on - strand at 5043.093 kb on + strand at 5043.093 kb on + strand at 5043.094 kb on - strand at 5043.094 kb on - strand at 5043.094 kb on - strand at 5043.132 kb on + strand at 5043.132 kb on + strand at 5043.132 kb on + strand at 5043.132 kb on + strand at 5043.132 kb on + strand at 5043.133 kb on - strand at 5043.133 kb on - strand at 5043.133 kb on - strand at 5043.133 kb on - strand at 5043.133 kb on - strand at 5043.158 kb on + strand at 5043.158 kb on + strand at 5043.158 kb on + strand at 5043.158 kb on + strand at 5043.159 kb on - strand at 5043.159 kb on - strand at 5043.159 kb on - strand at 5043.159 kb on - strand at 5043.159 kb on - strand at 5043.159 kb on - strand at 5043.439 kb on + strand, within AO353_22960 at 5043.439 kb on + strand, within AO353_22960 at 5043.440 kb on - strand, within AO353_22960 at 5043.440 kb on - strand, within AO353_22960 at 5043.440 kb on - strand, within AO353_22960 at 5043.440 kb on - strand, within AO353_22960 at 5043.440 kb on - strand, within AO353_22960 at 5043.440 kb on - strand, within AO353_22960 at 5043.440 kb on - strand, within AO353_22960 at 5043.467 kb on + strand, within AO353_22960 at 5043.730 kb on + strand, within AO353_22960 at 5043.730 kb on + strand, within AO353_22960 at 5043.730 kb on + strand, within AO353_22960 at 5043.730 kb on + strand, within AO353_22960 at 5043.731 kb on - strand, within AO353_22960 at 5043.731 kb on - strand, within AO353_22960 at 5043.731 kb on - strand, within AO353_22960 at 5043.731 kb on - strand, within AO353_22960 at 5043.731 kb on - strand, within AO353_22960 at 5043.808 kb on + strand at 5043.809 kb on - strand at 5043.809 kb on - strand at 5043.809 kb on - strand at 5043.838 kb on + strand at 5043.838 kb on + strand at 5043.839 kb on - strand at 5043.839 kb on - strand at 5043.846 kb on - strand at 5043.846 kb on - strand at 5043.865 kb on + strand at 5043.865 kb on + strand at 5043.866 kb on - strand at 5043.866 kb on - strand at 5043.866 kb on - strand at 5043.866 kb on - strand at 5043.866 kb on - strand at 5043.866 kb on - strand at 5043.913 kb on + strand at 5043.913 kb on + strand at 5043.914 kb on - strand at 5043.952 kb on + strand at 5043.952 kb on + strand at 5043.952 kb on + strand at 5043.952 kb on + strand at 5043.952 kb on + strand at 5043.952 kb on + strand at 5043.952 kb on + strand at 5043.952 kb on + strand at 5043.953 kb on - strand at 5043.953 kb on - strand at 5043.953 kb on - strand at 5043.953 kb on - strand at 5043.955 kb on - strand at 5043.982 kb on + strand, within AO353_22965 at 5043.983 kb on - strand, within AO353_22965 at 5043.983 kb on - strand, within AO353_22965 at 5043.983 kb on - strand, within AO353_22965 at 5043.983 kb on - strand, within AO353_22965 at 5044.234 kb on + strand, within AO353_22965 at 5044.234 kb on + strand, within AO353_22965 at 5044.234 kb on + strand, within AO353_22965 at 5044.234 kb on + strand, within AO353_22965 at 5044.234 kb on + strand, within AO353_22965 at 5044.235 kb on - strand, within AO353_22965 at 5044.235 kb on - strand, within AO353_22965 at 5044.235 kb on - strand, within AO353_22965 at 5044.247 kb on - strand, within AO353_22965 at 5044.253 kb on + strand, within AO353_22965 at 5044.282 kb on + strand, within AO353_22965 at 5044.283 kb on - strand, within AO353_22965 at 5044.283 kb on - strand, within AO353_22965 at 5044.582 kb on + strand, within AO353_22965 at 5044.582 kb on + strand, within AO353_22965 at 5044.582 kb on + strand, within AO353_22965 at 5044.582 kb on + strand, within AO353_22965 at 5044.583 kb on - strand, within AO353_22965 at 5044.583 kb on - strand, within AO353_22965 at 5044.583 kb on - strand, within AO353_22965 at 5044.583 kb on - strand, within AO353_22965 at 5044.583 kb on - strand, within AO353_22965 at 5044.583 kb on - strand, within AO353_22965 at 5044.583 kb on - strand, within AO353_22965 at 5044.583 kb on - strand, within AO353_22965 at 5044.622 kb on + strand, within AO353_22965 at 5044.623 kb on - strand, within AO353_22965 at 5044.623 kb on - strand, within AO353_22965 at 5044.630 kb on + strand, within AO353_22965 at 5044.630 kb on + strand, within AO353_22965 at 5044.630 kb on + strand, within AO353_22965 at 5044.630 kb on + strand, within AO353_22965 at 5044.631 kb on - strand, within AO353_22965 at 5044.631 kb on - strand, within AO353_22965 at 5044.631 kb on - strand, within AO353_22965 at 5044.631 kb on - strand, within AO353_22965 at 5044.862 kb on + strand, within AO353_22965 at 5044.862 kb on + strand, within AO353_22965 at 5045.116 kb on + strand at 5045.116 kb on + strand at 5045.116 kb on + strand at 5045.116 kb on + strand at 5045.117 kb on - strand at 5045.117 kb on - strand at 5045.117 kb on - strand at 5045.125 kb on + strand at 5045.125 kb on + strand at 5045.126 kb on - strand at 5045.126 kb on - strand at 5045.126 kb on - strand at 5045.170 kb on - strand at 5045.204 kb on + strand at 5045.204 kb on + strand at 5045.204 kb on + strand at 5045.204 kb on + strand at 5045.204 kb on + strand at 5045.204 kb on + strand at 5045.204 kb on + strand at 5045.204 kb on + strand at 5045.204 kb on + strand at 5045.205 kb on - strand at 5045.205 kb on - strand at 5045.205 kb on - strand at 5045.205 kb on - strand at 5045.213 kb on + strand at 5045.213 kb on + strand at 5045.213 kb on + strand at 5045.214 kb on - strand at 5045.214 kb on - strand at 5045.214 kb on - strand at 5045.214 kb on - strand at 5045.288 kb on + strand, within AO353_22970 at 5045.288 kb on + strand, within AO353_22970 at 5045.289 kb on - strand, within AO353_22970 at 5045.289 kb on - strand, within AO353_22970 at 5045.289 kb on - strand, within AO353_22970 at 5045.387 kb on + strand, within AO353_22970 at 5045.447 kb on + strand, within AO353_22970 at 5045.448 kb on - strand, within AO353_22970 at 5045.448 kb on - strand, within AO353_22970 at 5045.448 kb on - strand, within AO353_22970 at 5045.448 kb on - strand, within AO353_22970 at 5045.507 kb on + strand, within AO353_22970 at 5045.507 kb on + strand, within AO353_22970 at 5045.508 kb on - strand, within AO353_22970 at 5045.508 kb on - strand, within AO353_22970 at 5045.523 kb on + strand, within AO353_22970 at 5045.544 kb on - strand, within AO353_22970 at 5045.544 kb on - strand, within AO353_22970 at 5045.544 kb on - strand, within AO353_22970 at 5045.544 kb on - strand, within AO353_22970 at 5045.627 kb on + strand, within AO353_22970 at 5045.627 kb on + strand, within AO353_22970 at 5045.627 kb on + strand, within AO353_22970 at 5045.628 kb on - strand, within AO353_22970 at 5045.634 kb on + strand, within AO353_22970 at 5045.635 kb on - strand, within AO353_22970 at 5045.741 kb on + strand, within AO353_22970 at 5045.741 kb on + strand, within AO353_22970 at 5045.741 kb on + strand, within AO353_22970 at 5045.741 kb on + strand, within AO353_22970 at 5045.741 kb on + strand, within AO353_22970 at 5045.741 kb on + strand, within AO353_22970 at 5045.741 kb on + strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.742 kb on - strand, within AO353_22970 at 5045.814 kb on - strand, within AO353_22970 at 5045.814 kb on - strand, within AO353_22970 at 5045.873 kb on + strand, within AO353_22970 at 5045.874 kb on - strand, within AO353_22970 at 5045.909 kb on + strand, within AO353_22970 at 5045.909 kb on + strand, within AO353_22970 at 5045.909 kb on + strand, within AO353_22970 at 5045.910 kb on - strand, within AO353_22970 at 5045.910 kb on - strand, within AO353_22970 at 5045.933 kb on + strand at 5045.933 kb on + strand at 5045.934 kb on - strand at 5046.025 kb on + strand at 5046.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,042,849 + AO353_22955 0.79 +2.8 5,042,849 + AO353_22955 0.79 +0.1 5,043,007 + -0.1 5,043,007 + +2.8 5,043,007 + -0.6 5,043,008 - -0.5 5,043,091 + -1.3 5,043,091 + +2.3 5,043,091 + +0.5 5,043,092 - -1.9 5,043,092 - +0.6 5,043,093 + +0.6 5,043,093 + +2.2 5,043,094 - +0.0 5,043,094 - -2.1 5,043,094 - +0.7 5,043,132 + +0.0 5,043,132 + +0.6 5,043,132 + +1.9 5,043,132 + +5.4 5,043,132 + -0.1 5,043,133 - -1.5 5,043,133 - +0.0 5,043,133 - -0.1 5,043,133 - -1.1 5,043,133 - -1.4 5,043,158 + +0.8 5,043,158 + +0.0 5,043,158 + +2.4 5,043,158 + +2.2 5,043,159 - +1.5 5,043,159 - -1.4 5,043,159 - +0.9 5,043,159 - -0.2 5,043,159 - -0.6 5,043,159 - -2.9 5,043,439 + AO353_22960 0.43 +2.1 5,043,439 + AO353_22960 0.43 +3.5 5,043,440 - AO353_22960 0.43 +1.6 5,043,440 - AO353_22960 0.43 +0.1 5,043,440 - AO353_22960 0.43 -0.0 5,043,440 - AO353_22960 0.43 +0.3 5,043,440 - AO353_22960 0.43 +0.7 5,043,440 - AO353_22960 0.43 -0.6 5,043,440 - AO353_22960 0.43 -2.1 5,043,467 + AO353_22960 0.47 +1.4 5,043,730 + AO353_22960 0.84 -0.1 5,043,730 + AO353_22960 0.84 +0.4 5,043,730 + AO353_22960 0.84 +0.2 5,043,730 + AO353_22960 0.84 +1.2 5,043,731 - AO353_22960 0.84 -0.1 5,043,731 - AO353_22960 0.84 -0.6 5,043,731 - AO353_22960 0.84 -0.6 5,043,731 - AO353_22960 0.84 -1.1 5,043,731 - AO353_22960 0.84 -1.4 5,043,808 + +5.9 5,043,809 - -0.6 5,043,809 - +1.3 5,043,809 - -1.0 5,043,838 + -1.5 5,043,838 + +0.3 5,043,839 - +0.2 5,043,839 - -0.1 5,043,846 - -1.5 5,043,846 - -0.9 5,043,865 + -0.6 5,043,865 + -1.6 5,043,866 - -1.0 5,043,866 - -1.4 5,043,866 - +1.2 5,043,866 - -1.0 5,043,866 - +0.0 5,043,866 - +0.8 5,043,913 + -1.6 5,043,913 + +0.2 5,043,914 - +0.2 5,043,952 + -0.4 5,043,952 + +3.2 5,043,952 + -0.3 5,043,952 + +1.4 5,043,952 + -0.4 5,043,952 + +0.0 5,043,952 + -0.1 5,043,952 + -3.3 5,043,953 - -1.7 5,043,953 - +1.8 5,043,953 - +0.0 5,043,953 - +0.6 5,043,955 - -0.6 5,043,982 + AO353_22965 0.11 +0.4 5,043,983 - AO353_22965 0.11 -1.0 5,043,983 - AO353_22965 0.11 -1.1 5,043,983 - AO353_22965 0.11 +1.6 5,043,983 - AO353_22965 0.11 -0.6 5,044,234 + AO353_22965 0.31 -0.8 5,044,234 + AO353_22965 0.31 +0.7 5,044,234 + AO353_22965 0.31 -1.0 5,044,234 + AO353_22965 0.31 +0.9 5,044,234 + AO353_22965 0.31 +1.0 5,044,235 - AO353_22965 0.31 -0.4 5,044,235 - AO353_22965 0.31 -1.7 5,044,235 - AO353_22965 0.31 -0.4 5,044,247 - AO353_22965 0.32 +0.4 5,044,253 + AO353_22965 0.32 -0.4 5,044,282 + AO353_22965 0.35 -0.4 5,044,283 - AO353_22965 0.35 -1.3 5,044,283 - AO353_22965 0.35 -2.1 5,044,582 + AO353_22965 0.58 -1.3 5,044,582 + AO353_22965 0.58 -1.0 5,044,582 + AO353_22965 0.58 -0.5 5,044,582 + AO353_22965 0.58 -0.4 5,044,583 - AO353_22965 0.58 -0.6 5,044,583 - AO353_22965 0.58 -1.5 5,044,583 - AO353_22965 0.58 -1.3 5,044,583 - AO353_22965 0.58 -0.1 5,044,583 - AO353_22965 0.58 -1.4 5,044,583 - AO353_22965 0.58 -0.6 5,044,583 - AO353_22965 0.58 -1.5 5,044,583 - AO353_22965 0.58 -1.1 5,044,622 + AO353_22965 0.61 -0.1 5,044,623 - AO353_22965 0.61 +0.5 5,044,623 - AO353_22965 0.61 +1.0 5,044,630 + AO353_22965 0.62 -0.4 5,044,630 + AO353_22965 0.62 -0.3 5,044,630 + AO353_22965 0.62 +0.6 5,044,630 + AO353_22965 0.62 -2.0 5,044,631 - AO353_22965 0.62 -1.5 5,044,631 - AO353_22965 0.62 +0.9 5,044,631 - AO353_22965 0.62 +0.7 5,044,631 - AO353_22965 0.62 -0.1 5,044,862 + AO353_22965 0.80 -0.6 5,044,862 + AO353_22965 0.80 -1.3 5,045,116 + -0.8 5,045,116 + -0.1 5,045,116 + +0.6 5,045,116 + -2.3 5,045,117 - +2.5 5,045,117 - +1.0 5,045,117 - -0.8 5,045,125 + +0.2 5,045,125 + +0.2 5,045,126 - -0.3 5,045,126 - +0.4 5,045,126 - -0.8 5,045,170 - -1.7 5,045,204 + +0.6 5,045,204 + +1.7 5,045,204 + -1.0 5,045,204 + +0.1 5,045,204 + -1.1 5,045,204 + -0.6 5,045,204 + +1.2 5,045,204 + -0.6 5,045,204 + -0.5 5,045,205 - +0.4 5,045,205 - +0.1 5,045,205 - +0.4 5,045,205 - -0.3 5,045,213 + +1.0 5,045,213 + -1.7 5,045,213 + -1.4 5,045,214 - +1.7 5,045,214 - -0.3 5,045,214 - -0.5 5,045,214 - +0.2 5,045,288 + AO353_22970 0.11 +1.1 5,045,288 + AO353_22970 0.11 +1.9 5,045,289 - AO353_22970 0.11 +0.2 5,045,289 - AO353_22970 0.11 +1.2 5,045,289 - AO353_22970 0.11 -0.1 5,045,387 + AO353_22970 0.23 +1.6 5,045,447 + AO353_22970 0.30 +0.6 5,045,448 - AO353_22970 0.30 -1.0 5,045,448 - AO353_22970 0.30 -1.1 5,045,448 - AO353_22970 0.30 +0.9 5,045,448 - AO353_22970 0.30 +0.6 5,045,507 + AO353_22970 0.38 -0.8 5,045,507 + AO353_22970 0.38 +0.7 5,045,508 - AO353_22970 0.38 -0.6 5,045,508 - AO353_22970 0.38 -1.5 5,045,523 + AO353_22970 0.40 +1.5 5,045,544 - AO353_22970 0.42 -1.2 5,045,544 - AO353_22970 0.42 -1.4 5,045,544 - AO353_22970 0.42 -0.1 5,045,544 - AO353_22970 0.42 -1.0 5,045,627 + AO353_22970 0.53 +0.9 5,045,627 + AO353_22970 0.53 +0.6 5,045,627 + AO353_22970 0.53 -2.5 5,045,628 - AO353_22970 0.53 -0.2 5,045,634 + AO353_22970 0.54 +0.2 5,045,635 - AO353_22970 0.54 -0.8 5,045,741 + AO353_22970 0.67 -0.9 5,045,741 + AO353_22970 0.67 +0.0 5,045,741 + AO353_22970 0.67 +0.4 5,045,741 + AO353_22970 0.67 +0.0 5,045,741 + AO353_22970 0.67 -1.3 5,045,741 + AO353_22970 0.67 -0.4 5,045,741 + AO353_22970 0.67 -1.0 5,045,742 - AO353_22970 0.67 +2.4 5,045,742 - AO353_22970 0.67 +0.4 5,045,742 - AO353_22970 0.67 -1.1 5,045,742 - AO353_22970 0.67 -1.1 5,045,742 - AO353_22970 0.67 +0.9 5,045,742 - AO353_22970 0.67 -1.5 5,045,742 - AO353_22970 0.67 -1.4 5,045,742 - AO353_22970 0.67 -0.4 5,045,742 - AO353_22970 0.67 -0.3 5,045,742 - AO353_22970 0.67 +0.2 5,045,814 - AO353_22970 0.76 +0.4 5,045,814 - AO353_22970 0.76 +0.0 5,045,873 + AO353_22970 0.83 +0.4 5,045,874 - AO353_22970 0.83 +1.9 5,045,909 + AO353_22970 0.88 -1.0 5,045,909 + AO353_22970 0.88 +0.2 5,045,909 + AO353_22970 0.88 -1.4 5,045,910 - AO353_22970 0.88 +0.1 5,045,910 - AO353_22970 0.88 -3.1 5,045,933 + +2.2 5,045,933 + -0.1 5,045,934 - +0.3 5,046,025 + -1.8 5,046,042 - -0.6
Or see this region's nucleotide sequence