Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_12355 and AO353_12360 are separated by 25 nucleotides AO353_12360 and AO353_12365 overlap by 8 nucleotides AO353_12365 and AO353_12370 are separated by 81 nucleotides AO353_12370 and AO353_12375 overlap by 4 nucleotides AO353_12375 and AO353_12380 overlap by 1 nucleotides
AO353_12355: AO353_12355 - preprotein translocase subunit SecA, at 2,719,580 to 2,719,861
_12355
AO353_12360: AO353_12360 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 2,719,887 to 2,720,219
_12360
AO353_12365: AO353_12365 - phosphoribosyl-AMP cyclohydrolase, at 2,720,212 to 2,720,610
_12365
AO353_12370: AO353_12370 - ubiquinone biosynthesis protein UbiB, at 2,720,692 to 2,722,296
_12370
AO353_12375: AO353_12375 - sterol-binding protein, at 2,722,293 to 2,722,916
_12375
AO353_12380: AO353_12380 - ubiquinone biosynthesis methyltransferase UbiE, at 2,722,916 to 2,723,686
_12380
Position (kb)
2720
2721
2722
2723 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2719.878 kb on - strand at 2719.997 kb on + strand, within AO353_12360 at 2719.997 kb on + strand, within AO353_12360 at 2719.997 kb on + strand, within AO353_12360 at 2719.997 kb on + strand, within AO353_12360 at 2719.997 kb on + strand, within AO353_12360 at 2719.998 kb on - strand, within AO353_12360 at 2719.998 kb on - strand, within AO353_12360 at 2719.998 kb on - strand, within AO353_12360 at 2719.998 kb on - strand, within AO353_12360 at 2719.998 kb on - strand, within AO353_12360 at 2719.998 kb on - strand, within AO353_12360 at 2719.998 kb on - strand, within AO353_12360 at 2719.998 kb on - strand, within AO353_12360 at 2720.000 kb on + strand, within AO353_12360 at 2720.000 kb on + strand, within AO353_12360 at 2720.000 kb on + strand, within AO353_12360 at 2720.001 kb on - strand, within AO353_12360 at 2720.001 kb on - strand, within AO353_12360 at 2720.001 kb on - strand, within AO353_12360 at 2720.001 kb on - strand, within AO353_12360 at 2720.001 kb on - strand, within AO353_12360 at 2720.001 kb on - strand, within AO353_12360 at 2720.026 kb on + strand, within AO353_12360 at 2720.027 kb on - strand, within AO353_12360 at 2720.027 kb on - strand, within AO353_12360 at 2720.031 kb on + strand, within AO353_12360 at 2720.031 kb on + strand, within AO353_12360 at 2720.031 kb on + strand, within AO353_12360 at 2720.031 kb on + strand at 2720.031 kb on + strand, within AO353_12360 at 2720.031 kb on + strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.032 kb on - strand, within AO353_12360 at 2720.134 kb on + strand, within AO353_12360 at 2720.135 kb on - strand, within AO353_12360 at 2720.135 kb on - strand, within AO353_12360 at 2720.149 kb on + strand, within AO353_12360 at 2720.149 kb on + strand, within AO353_12360 at 2720.149 kb on + strand, within AO353_12360 at 2720.149 kb on + strand, within AO353_12360 at 2720.149 kb on + strand, within AO353_12360 at 2720.150 kb on - strand, within AO353_12360 at 2720.150 kb on - strand, within AO353_12360 at 2720.150 kb on - strand, within AO353_12360 at 2720.150 kb on - strand, within AO353_12360 at 2720.175 kb on - strand, within AO353_12360 at 2720.234 kb on + strand at 2720.234 kb on + strand at 2720.235 kb on - strand at 2720.235 kb on - strand at 2720.235 kb on - strand at 2720.235 kb on - strand at 2720.235 kb on - strand at 2720.292 kb on - strand, within AO353_12365 at 2720.292 kb on - strand, within AO353_12365 at 2720.462 kb on + strand, within AO353_12365 at 2720.463 kb on - strand, within AO353_12365 at 2720.463 kb on - strand, within AO353_12365 at 2720.463 kb on - strand, within AO353_12365 at 2720.541 kb on + strand, within AO353_12365 at 2720.541 kb on + strand, within AO353_12365 at 2720.541 kb on + strand, within AO353_12365 at 2720.541 kb on + strand, within AO353_12365 at 2720.542 kb on - strand, within AO353_12365 at 2720.542 kb on - strand, within AO353_12365 at 2720.542 kb on - strand, within AO353_12365 at 2720.643 kb on + strand at 2720.644 kb on - strand at 2722.559 kb on - strand, within AO353_12375
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 2,719,878 - +1.1 2,719,997 + AO353_12360 0.33 +4.2 2,719,997 + AO353_12360 0.33 +3.3 2,719,997 + AO353_12360 0.33 +4.7 2,719,997 + AO353_12360 0.33 +4.1 2,719,997 + AO353_12360 0.33 +3.9 2,719,998 - AO353_12360 0.33 -0.8 2,719,998 - AO353_12360 0.33 -1.4 2,719,998 - AO353_12360 0.33 -0.5 2,719,998 - AO353_12360 0.33 -0.4 2,719,998 - AO353_12360 0.33 +0.2 2,719,998 - AO353_12360 0.33 -1.7 2,719,998 - AO353_12360 0.33 +0.7 2,719,998 - AO353_12360 0.33 +0.7 2,720,000 + AO353_12360 0.34 +1.8 2,720,000 + AO353_12360 0.34 +2.4 2,720,000 + AO353_12360 0.34 +3.8 2,720,001 - AO353_12360 0.34 -0.7 2,720,001 - AO353_12360 0.34 +1.1 2,720,001 - AO353_12360 0.34 -0.5 2,720,001 - AO353_12360 0.34 -0.9 2,720,001 - AO353_12360 0.34 +0.4 2,720,001 - AO353_12360 0.34 +2.3 2,720,026 + AO353_12360 0.42 +2.9 2,720,027 - AO353_12360 0.42 -0.4 2,720,027 - AO353_12360 0.42 -0.7 2,720,031 + AO353_12360 0.43 +2.9 2,720,031 + AO353_12360 0.43 -0.8 2,720,031 + AO353_12360 0.43 +4.2 2,720,031 + +4.3 2,720,031 + AO353_12360 0.43 +1.5 2,720,031 + AO353_12360 0.43 +2.5 2,720,032 - AO353_12360 0.44 +0.7 2,720,032 - AO353_12360 0.44 +0.2 2,720,032 - AO353_12360 0.44 +0.8 2,720,032 - AO353_12360 0.44 -1.3 2,720,032 - AO353_12360 0.44 -1.5 2,720,032 - AO353_12360 0.44 +0.2 2,720,032 - AO353_12360 0.44 +1.2 2,720,032 - AO353_12360 0.44 +0.7 2,720,032 - AO353_12360 0.44 -0.0 2,720,134 + AO353_12360 0.74 +2.2 2,720,135 - AO353_12360 0.74 -0.9 2,720,135 - AO353_12360 0.74 -1.2 2,720,149 + AO353_12360 0.79 +4.0 2,720,149 + AO353_12360 0.79 +2.6 2,720,149 + AO353_12360 0.79 +2.3 2,720,149 + AO353_12360 0.79 +5.4 2,720,149 + AO353_12360 0.79 +1.8 2,720,150 - AO353_12360 0.79 +1.2 2,720,150 - AO353_12360 0.79 +1.7 2,720,150 - AO353_12360 0.79 -0.1 2,720,150 - AO353_12360 0.79 +0.7 2,720,175 - AO353_12360 0.86 +1.4 2,720,234 + +4.8 2,720,234 + +3.0 2,720,235 - -1.1 2,720,235 - -0.5 2,720,235 - +0.9 2,720,235 - -0.3 2,720,235 - -0.1 2,720,292 - AO353_12365 0.20 +0.7 2,720,292 - AO353_12365 0.20 +0.5 2,720,462 + AO353_12365 0.63 +0.7 2,720,463 - AO353_12365 0.63 +0.8 2,720,463 - AO353_12365 0.63 +1.0 2,720,463 - AO353_12365 0.63 +1.4 2,720,541 + AO353_12365 0.82 +0.4 2,720,541 + AO353_12365 0.82 +3.6 2,720,541 + AO353_12365 0.82 +1.7 2,720,541 + AO353_12365 0.82 -0.5 2,720,542 - AO353_12365 0.83 +1.0 2,720,542 - AO353_12365 0.83 +1.1 2,720,542 - AO353_12365 0.83 +0.3 2,720,643 + +2.0 2,720,644 - +1.7 2,722,559 - AO353_12375 0.43 +4.1
Or see this region's nucleotide sequence