Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_07370 and AO353_07375 are separated by 199 nucleotides AO353_07375 and AO353_07380 are separated by 489 nucleotides AO353_07380 and AO353_07385 are separated by 67 nucleotides
AO353_07370: AO353_07370 - tRNA threonylcarbamoyl adenosine modification protein TsaD, at 1,618,530 to 1,619,555
_07370
AO353_07375: AO353_07375 - 30S ribosomal protein S21, at 1,619,755 to 1,619,970
_07375
AO353_07380: AO353_07380 - DNA primase, at 1,620,460 to 1,622,421
_07380
AO353_07385: AO353_07385 - RNA polymerase subunit sigma-70, at 1,622,489 to 1,624,336
_07385
Position (kb)
1620
1621
1622
1623 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1619.599 kb on - strand at 1619.599 kb on - strand at 1620.022 kb on + strand at 1620.022 kb on + strand at 1620.022 kb on + strand at 1620.226 kb on + strand at 1620.226 kb on + strand at 1620.226 kb on + strand at 1620.226 kb on + strand at 1620.226 kb on + strand at 1620.226 kb on + strand at 1620.226 kb on + strand at 1620.226 kb on + strand at 1620.233 kb on + strand at 1620.304 kb on + strand at 1620.304 kb on + strand at 1620.328 kb on + strand at 1620.328 kb on + strand at 1620.328 kb on + strand at 1620.328 kb on + strand at 1620.358 kb on + strand at 1620.358 kb on + strand at 1620.394 kb on + strand at 1620.394 kb on + strand at 1620.394 kb on + strand at 1620.394 kb on + strand at 1620.394 kb on + strand at 1620.394 kb on + strand at 1620.394 kb on + strand at 1620.394 kb on + strand at 1622.375 kb on + strand at 1622.375 kb on + strand at 1622.375 kb on + strand at 1622.427 kb on + strand at 1622.431 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,619,599 - +1.8 1,619,599 - +0.2 1,620,022 + +0.8 1,620,022 + +0.7 1,620,022 + +2.8 1,620,226 + -1.1 1,620,226 + -0.4 1,620,226 + +0.8 1,620,226 + -0.0 1,620,226 + +1.2 1,620,226 + -0.2 1,620,226 + -0.6 1,620,226 + -0.0 1,620,233 + -0.3 1,620,304 + -0.8 1,620,304 + +2.0 1,620,328 + +0.2 1,620,328 + -0.6 1,620,328 + -0.7 1,620,328 + -1.9 1,620,358 + +1.7 1,620,358 + -1.0 1,620,394 + +2.0 1,620,394 + -2.2 1,620,394 + -0.9 1,620,394 + -0.3 1,620,394 + +0.4 1,620,394 + -1.3 1,620,394 + -0.5 1,620,394 + -0.6 1,622,375 + -0.4 1,622,375 + -1.9 1,622,375 + +0.4 1,622,427 + +0.2 1,622,431 + +2.8
Or see this region's nucleotide sequence