Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_00920 and AO353_00925 are separated by 262 nucleotides AO353_00925 and AO353_00930 are separated by 170 nucleotides AO353_00930 and AO353_00935 are separated by 174 nucleotides
AO353_00920: AO353_00920 - alpha-L-glutamate ligase, at 209,309 to 210,295
_00920
AO353_00925: AO353_00925 - aminodeoxychorismate synthase component I, at 210,558 to 211,901
_00925
AO353_00930: AO353_00930 - phosphoserine phosphatase, at 212,072 to 212,689
_00930
AO353_00935: AO353_00935 - phosphoadenosine phosphosulfate reductase, at 212,864 to 213,598
_00935
Position (kb) 210
211
212 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 209.699 kb on + strand, within AO353_00920 at 209.699 kb on + strand, within AO353_00920 at 209.699 kb on + strand, within AO353_00920 at 209.699 kb on + strand, within AO353_00920 at 209.700 kb on - strand, within AO353_00920 at 209.777 kb on + strand, within AO353_00920 at 209.777 kb on + strand, within AO353_00920 at 209.778 kb on - strand, within AO353_00920 at 209.778 kb on - strand, within AO353_00920 at 209.986 kb on - strand, within AO353_00920 at 209.986 kb on - strand, within AO353_00920 at 210.005 kb on + strand, within AO353_00920 at 210.005 kb on + strand, within AO353_00920 at 210.041 kb on + strand, within AO353_00920 at 210.041 kb on + strand, within AO353_00920 at 210.041 kb on + strand, within AO353_00920 at 210.041 kb on + strand, within AO353_00920 at 210.041 kb on + strand, within AO353_00920 at 210.041 kb on + strand, within AO353_00920 at 210.042 kb on - strand, within AO353_00920 at 210.042 kb on - strand, within AO353_00920 at 210.063 kb on - strand, within AO353_00920 at 210.063 kb on - strand, within AO353_00920 at 210.294 kb on - strand at 210.294 kb on - strand at 210.343 kb on + strand at 210.343 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.364 kb on + strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.365 kb on - strand at 210.390 kb on + strand at 210.391 kb on - strand at 210.471 kb on + strand at 210.471 kb on + strand at 210.471 kb on + strand at 210.471 kb on + strand at 210.471 kb on + strand at 210.471 kb on + strand at 210.471 kb on + strand at 210.471 kb on + strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.472 kb on - strand at 210.494 kb on - strand at 210.516 kb on + strand at 210.516 kb on + strand at 210.516 kb on + strand at 210.517 kb on - strand at 210.517 kb on - strand at 210.517 kb on - strand at 210.517 kb on - strand at 210.517 kb on - strand at 210.575 kb on - strand at 210.575 kb on - strand at 210.575 kb on - strand at 210.575 kb on - strand at 210.575 kb on - strand at 210.643 kb on + strand at 210.643 kb on + strand at 210.644 kb on - strand at 210.644 kb on - strand at 210.644 kb on - strand at 210.644 kb on - strand at 210.684 kb on + strand at 210.684 kb on + strand at 210.684 kb on + strand at 210.684 kb on + strand at 210.685 kb on - strand at 210.817 kb on + strand, within AO353_00925 at 210.817 kb on + strand, within AO353_00925 at 210.817 kb on + strand, within AO353_00925 at 210.817 kb on + strand, within AO353_00925 at 210.818 kb on - strand, within AO353_00925 at 210.818 kb on - strand, within AO353_00925 at 210.844 kb on - strand, within AO353_00925 at 210.933 kb on + strand, within AO353_00925 at 210.933 kb on + strand, within AO353_00925 at 210.934 kb on - strand, within AO353_00925 at 210.934 kb on - strand, within AO353_00925 at 210.934 kb on - strand, within AO353_00925 at 211.110 kb on + strand, within AO353_00925 at 211.111 kb on - strand, within AO353_00925 at 211.140 kb on + strand, within AO353_00925 at 211.140 kb on + strand, within AO353_00925 at 211.140 kb on + strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.141 kb on - strand, within AO353_00925 at 211.161 kb on + strand, within AO353_00925 at 211.162 kb on - strand, within AO353_00925 at 211.162 kb on - strand, within AO353_00925 at 211.339 kb on + strand, within AO353_00925 at 211.339 kb on + strand, within AO353_00925 at 211.339 kb on + strand, within AO353_00925 at 211.340 kb on - strand, within AO353_00925 at 211.340 kb on - strand, within AO353_00925 at 211.340 kb on - strand, within AO353_00925 at 211.340 kb on - strand, within AO353_00925 at 211.704 kb on + strand, within AO353_00925 at 211.722 kb on + strand, within AO353_00925 at 211.722 kb on + strand, within AO353_00925 at 211.722 kb on + strand, within AO353_00925 at 211.723 kb on - strand, within AO353_00925 at 211.723 kb on - strand, within AO353_00925 at 211.723 kb on - strand, within AO353_00925 at 211.723 kb on - strand, within AO353_00925 at 211.723 kb on - strand, within AO353_00925 at 211.732 kb on + strand, within AO353_00925 at 211.732 kb on + strand, within AO353_00925 at 211.766 kb on + strand, within AO353_00925 at 211.766 kb on + strand, within AO353_00925 at 211.766 kb on + strand, within AO353_00925 at 211.767 kb on - strand at 211.767 kb on - strand at 211.846 kb on - strand at 211.993 kb on + strand at 211.994 kb on - strand at 211.994 kb on - strand at 212.002 kb on - strand at 212.002 kb on - strand at 212.073 kb on + strand at 212.073 kb on + strand at 212.073 kb on + strand at 212.229 kb on + strand, within AO353_00930 at 212.229 kb on + strand, within AO353_00930 at 212.229 kb on + strand, within AO353_00930 at 212.229 kb on + strand, within AO353_00930 at 212.230 kb on - strand, within AO353_00930 at 212.230 kb on - strand, within AO353_00930 at 212.259 kb on + strand, within AO353_00930 at 212.259 kb on + strand, within AO353_00930 at 212.259 kb on + strand, within AO353_00930 at 212.259 kb on + strand, within AO353_00930 at 212.260 kb on - strand, within AO353_00930 at 212.260 kb on - strand, within AO353_00930 at 212.260 kb on - strand, within AO353_00930 at 212.260 kb on - strand, within AO353_00930 at 212.410 kb on - strand, within AO353_00930 at 212.585 kb on - strand, within AO353_00930 at 212.706 kb on + strand at 212.706 kb on + strand at 212.707 kb on - strand at 212.707 kb on - strand at 212.707 kb on - strand at 212.748 kb on + strand at 212.758 kb on + strand at 212.758 kb on + strand at 212.758 kb on + strand at 212.758 kb on + strand at 212.758 kb on + strand at 212.758 kb on + strand at 212.759 kb on - strand at 212.808 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.822 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand at 212.824 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 209,699 + AO353_00920 0.40 +3.6 209,699 + AO353_00920 0.40 +2.1 209,699 + AO353_00920 0.40 +1.9 209,699 + AO353_00920 0.40 +1.5 209,700 - AO353_00920 0.40 +1.9 209,777 + AO353_00920 0.47 +0.9 209,777 + AO353_00920 0.47 +3.3 209,778 - AO353_00920 0.48 +3.6 209,778 - AO353_00920 0.48 +1.9 209,986 - AO353_00920 0.69 +3.5 209,986 - AO353_00920 0.69 +2.2 210,005 + AO353_00920 0.71 +3.9 210,005 + AO353_00920 0.71 +2.1 210,041 + AO353_00920 0.74 +2.9 210,041 + AO353_00920 0.74 +3.3 210,041 + AO353_00920 0.74 +3.3 210,041 + AO353_00920 0.74 +2.7 210,041 + AO353_00920 0.74 +2.7 210,041 + AO353_00920 0.74 -0.7 210,042 - AO353_00920 0.74 +1.9 210,042 - AO353_00920 0.74 +3.7 210,063 - AO353_00920 0.76 +3.1 210,063 - AO353_00920 0.76 +1.7 210,294 - -1.3 210,294 - -1.4 210,343 + -0.5 210,343 + -1.6 210,364 + -0.6 210,364 + +0.1 210,364 + +0.1 210,364 + +0.0 210,364 + +0.2 210,364 + -0.7 210,364 + +0.3 210,364 + -1.7 210,364 + -0.7 210,364 + +1.2 210,364 + -0.8 210,364 + +1.3 210,365 - +0.5 210,365 - +0.8 210,365 - -0.2 210,365 - +0.5 210,365 - -1.0 210,365 - +0.1 210,365 - -0.6 210,365 - +0.6 210,365 - +1.6 210,365 - -2.7 210,365 - -0.2 210,365 - +0.8 210,365 - -2.3 210,365 - -1.0 210,365 - +1.7 210,390 + -0.1 210,391 - +0.9 210,471 + +0.7 210,471 + +1.3 210,471 + -1.7 210,471 + +0.6 210,471 + +0.3 210,471 + +0.5 210,471 + +0.3 210,471 + +0.3 210,472 - -0.7 210,472 - -0.4 210,472 - -0.5 210,472 - -2.2 210,472 - +0.1 210,472 - +0.3 210,472 - -1.1 210,472 - -1.1 210,472 - +1.0 210,472 - -1.7 210,472 - +0.6 210,472 - -0.3 210,472 - -3.1 210,472 - +0.8 210,472 - -0.7 210,494 - -1.8 210,516 + -0.8 210,516 + +2.3 210,516 + -1.2 210,517 - -0.1 210,517 - +0.0 210,517 - -0.8 210,517 - -0.3 210,517 - +0.5 210,575 - +1.0 210,575 - -1.7 210,575 - +1.5 210,575 - +0.3 210,575 - +1.8 210,643 + +0.2 210,643 + +0.0 210,644 - +0.0 210,644 - -0.6 210,644 - +0.2 210,644 - +0.8 210,684 + +0.3 210,684 + -1.7 210,684 + +0.5 210,684 + -1.1 210,685 - -0.2 210,817 + AO353_00925 0.19 -1.1 210,817 + AO353_00925 0.19 -0.9 210,817 + AO353_00925 0.19 -0.2 210,817 + AO353_00925 0.19 -1.1 210,818 - AO353_00925 0.19 +0.5 210,818 - AO353_00925 0.19 -0.2 210,844 - AO353_00925 0.21 -0.2 210,933 + AO353_00925 0.28 -0.9 210,933 + AO353_00925 0.28 -1.2 210,934 - AO353_00925 0.28 +0.9 210,934 - AO353_00925 0.28 +2.3 210,934 - AO353_00925 0.28 -1.6 211,110 + AO353_00925 0.41 -1.5 211,111 - AO353_00925 0.41 -0.1 211,140 + AO353_00925 0.43 -0.1 211,140 + AO353_00925 0.43 -1.2 211,140 + AO353_00925 0.43 -2.9 211,141 - AO353_00925 0.43 +1.0 211,141 - AO353_00925 0.43 -1.6 211,141 - AO353_00925 0.43 -0.7 211,141 - AO353_00925 0.43 -1.1 211,141 - AO353_00925 0.43 -1.7 211,141 - AO353_00925 0.43 +0.3 211,141 - AO353_00925 0.43 +1.5 211,141 - AO353_00925 0.43 +0.0 211,141 - AO353_00925 0.43 +0.3 211,141 - AO353_00925 0.43 +1.7 211,141 - AO353_00925 0.43 -0.8 211,161 + AO353_00925 0.45 +0.2 211,162 - AO353_00925 0.45 +1.1 211,162 - AO353_00925 0.45 +0.1 211,339 + AO353_00925 0.58 +0.8 211,339 + AO353_00925 0.58 -1.6 211,339 + AO353_00925 0.58 +1.3 211,340 - AO353_00925 0.58 -0.1 211,340 - AO353_00925 0.58 -1.2 211,340 - AO353_00925 0.58 -0.8 211,340 - AO353_00925 0.58 -0.7 211,704 + AO353_00925 0.85 +0.0 211,722 + AO353_00925 0.87 +0.6 211,722 + AO353_00925 0.87 -0.6 211,722 + AO353_00925 0.87 +1.1 211,723 - AO353_00925 0.87 +0.3 211,723 - AO353_00925 0.87 +0.0 211,723 - AO353_00925 0.87 +1.7 211,723 - AO353_00925 0.87 -0.5 211,723 - AO353_00925 0.87 +0.3 211,732 + AO353_00925 0.87 -1.5 211,732 + AO353_00925 0.87 +2.3 211,766 + AO353_00925 0.90 -1.4 211,766 + AO353_00925 0.90 -2.4 211,766 + AO353_00925 0.90 +0.0 211,767 - -0.2 211,767 - +0.0 211,846 - +0.9 211,993 + -1.6 211,994 - -0.5 211,994 - +0.3 212,002 - +0.6 212,002 - +0.1 212,073 + +0.2 212,073 + +2.3 212,073 + -0.5 212,229 + AO353_00930 0.25 +1.0 212,229 + AO353_00930 0.25 -2.2 212,229 + AO353_00930 0.25 -1.4 212,229 + AO353_00930 0.25 +0.8 212,230 - AO353_00930 0.26 -1.0 212,230 - AO353_00930 0.26 -0.5 212,259 + AO353_00930 0.30 -0.8 212,259 + AO353_00930 0.30 +0.4 212,259 + AO353_00930 0.30 +0.9 212,259 + AO353_00930 0.30 -0.7 212,260 - AO353_00930 0.30 -0.5 212,260 - AO353_00930 0.30 +1.0 212,260 - AO353_00930 0.30 +0.0 212,260 - AO353_00930 0.30 +0.3 212,410 - AO353_00930 0.55 -0.2 212,585 - AO353_00930 0.83 -1.5 212,706 + +1.6 212,706 + -1.0 212,707 - -0.2 212,707 - -1.7 212,707 - -0.8 212,748 + +0.3 212,758 + -0.9 212,758 + -1.0 212,758 + -0.5 212,758 + +0.0 212,758 + +0.3 212,758 + -1.1 212,759 - +0.7 212,808 + +0.7 212,822 + -0.1 212,822 + -0.7 212,822 + +0.3 212,822 + -0.2 212,822 + +0.2 212,822 + -0.5 212,822 + -0.2 212,822 + -1.1 212,822 + -0.7 212,822 + +0.3 212,822 + +2.3 212,822 + +0.9 212,822 + +2.3 212,824 + -0.2 212,824 + -0.8 212,824 + -0.2 212,824 + +0.1 212,824 + +0.9 212,824 + +1.3 212,824 + +0.1 212,824 + +1.1 212,824 + +1.3 212,824 + -2.4 212,824 + +1.0 212,824 + +0.3 212,824 + -1.1
Or see this region's nucleotide sequence