Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS08235

Experiment: formate McAA B12 dropout

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS08225 and MMJJ_RS08230 are separated by 68 nucleotidesMMJJ_RS08230 and MMJJ_RS08235 are separated by 142 nucleotidesMMJJ_RS08235 and MMJJ_RS08240 are separated by 80 nucleotidesMMJJ_RS08240 and MMJJ_RS08245 are separated by 28 nucleotides MMJJ_RS08225: MMJJ_RS08225 - peptidylprolyl isomerase, at 1,514,997 to 1,515,449 _RS08225 MMJJ_RS08230: MMJJ_RS08230 - ribose-5-phosphate isomerase RpiA, at 1,515,518 to 1,516,237 _RS08230 MMJJ_RS08235: MMJJ_RS08235 - UPF0147 family protein, at 1,516,380 to 1,516,655 _RS08235 MMJJ_RS08240: MMJJ_RS08240 - L-fuculose phosphate aldolase, at 1,516,736 to 1,517,278 _RS08240 MMJJ_RS08245: MMJJ_RS08245 - ATP-dependent protease LonB, at 1,517,307 to 1,519,385 _RS08245 Position (kb) 1516 1517Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1515.435 kb on + strandat 1515.436 kb on - strandat 1515.437 kb on + strandat 1515.462 kb on + strandat 1515.470 kb on - strandat 1515.470 kb on - strandat 1515.489 kb on - strandat 1515.498 kb on + strandat 1515.498 kb on + strandat 1515.524 kb on + strandat 1515.550 kb on + strandat 1515.699 kb on + strand, within MMJJ_RS08230at 1515.700 kb on - strand, within MMJJ_RS08230at 1515.715 kb on - strand, within MMJJ_RS08230at 1515.716 kb on + strand, within MMJJ_RS08230at 1515.802 kb on + strand, within MMJJ_RS08230at 1515.986 kb on - strand, within MMJJ_RS08230at 1516.181 kb on + strandat 1516.225 kb on + strandat 1516.250 kb on - strandat 1516.254 kb on - strandat 1516.283 kb on - strandat 1516.283 kb on - strandat 1516.283 kb on - strandat 1516.284 kb on + strandat 1516.284 kb on + strandat 1516.296 kb on + strandat 1516.317 kb on - strandat 1516.317 kb on - strandat 1516.318 kb on + strandat 1516.318 kb on + strandat 1516.327 kb on - strandat 1516.328 kb on + strandat 1516.328 kb on + strandat 1516.328 kb on + strandat 1516.350 kb on - strandat 1516.354 kb on - strandat 1516.355 kb on + strandat 1516.458 kb on + strand, within MMJJ_RS08235at 1516.511 kb on + strand, within MMJJ_RS08235at 1516.511 kb on + strand, within MMJJ_RS08235at 1516.590 kb on + strand, within MMJJ_RS08235at 1516.653 kb on - strandat 1516.657 kb on - strandat 1516.672 kb on - strandat 1516.714 kb on - strandat 1516.715 kb on + strandat 1516.722 kb on - strandat 1516.754 kb on + strandat 1516.756 kb on - strandat 1516.756 kb on - strandat 1516.757 kb on + strandat 1516.758 kb on - strandat 1516.759 kb on + strandat 1516.759 kb on + strandat 1516.764 kb on + strandat 1516.782 kb on + strandat 1516.782 kb on + strandat 1516.800 kb on - strand, within MMJJ_RS08240at 1516.800 kb on - strand, within MMJJ_RS08240at 1516.801 kb on + strand, within MMJJ_RS08240at 1516.812 kb on + strand, within MMJJ_RS08240at 1516.813 kb on - strand, within MMJJ_RS08240at 1516.829 kb on + strand, within MMJJ_RS08240at 1516.853 kb on + strand, within MMJJ_RS08240at 1516.915 kb on + strand, within MMJJ_RS08240at 1516.915 kb on + strand, within MMJJ_RS08240at 1516.923 kb on - strand, within MMJJ_RS08240at 1516.923 kb on - strand, within MMJJ_RS08240at 1516.924 kb on + strand, within MMJJ_RS08240at 1516.924 kb on + strand, within MMJJ_RS08240at 1516.924 kb on + strand, within MMJJ_RS08240at 1516.924 kb on + strand, within MMJJ_RS08240at 1516.924 kb on + strand, within MMJJ_RS08240at 1516.924 kb on + strand, within MMJJ_RS08240at 1516.928 kb on - strand, within MMJJ_RS08240at 1516.970 kb on - strand, within MMJJ_RS08240at 1517.004 kb on - strand, within MMJJ_RS08240at 1517.004 kb on - strand, within MMJJ_RS08240at 1517.005 kb on + strand, within MMJJ_RS08240at 1517.024 kb on - strand, within MMJJ_RS08240at 1517.024 kb on - strand, within MMJJ_RS08240at 1517.052 kb on - strand, within MMJJ_RS08240at 1517.054 kb on - strand, within MMJJ_RS08240at 1517.072 kb on - strand, within MMJJ_RS08240at 1517.073 kb on + strand, within MMJJ_RS08240at 1517.083 kb on - strand, within MMJJ_RS08240at 1517.097 kb on + strand, within MMJJ_RS08240at 1517.156 kb on - strand, within MMJJ_RS08240at 1517.170 kb on - strand, within MMJJ_RS08240at 1517.171 kb on + strand, within MMJJ_RS08240at 1517.171 kb on + strand, within MMJJ_RS08240at 1517.173 kb on - strand, within MMJJ_RS08240at 1517.173 kb on - strand, within MMJJ_RS08240at 1517.173 kb on - strand, within MMJJ_RS08240at 1517.175 kb on - strand, within MMJJ_RS08240at 1517.175 kb on - strand, within MMJJ_RS08240at 1517.175 kb on - strand, within MMJJ_RS08240at 1517.175 kb on - strand, within MMJJ_RS08240at 1517.176 kb on + strand, within MMJJ_RS08240at 1517.176 kb on + strand, within MMJJ_RS08240at 1517.176 kb on + strand, within MMJJ_RS08240at 1517.176 kb on + strand, within MMJJ_RS08240at 1517.176 kb on + strand, within MMJJ_RS08240at 1517.197 kb on - strand, within MMJJ_RS08240at 1517.201 kb on - strand, within MMJJ_RS08240at 1517.201 kb on - strand, within MMJJ_RS08240at 1517.202 kb on + strand, within MMJJ_RS08240at 1517.226 kb on - strandat 1517.226 kb on - strandat 1517.238 kb on - strandat 1517.238 kb on - strandat 1517.240 kb on - strandat 1517.240 kb on - strandat 1517.244 kb on + strandat 1517.244 kb on + strandat 1517.244 kb on + strandat 1517.259 kb on + strandat 1517.304 kb on + strandat 1517.304 kb on + strandat 1517.304 kb on + strandat 1517.310 kb on - strandat 1517.310 kb on - strandat 1517.311 kb on + strandat 1517.316 kb on + strandat 1517.316 kb on + strandat 1517.316 kb on + strandat 1517.332 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction formate McAA B12 dropout
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1,515,435 + +2.5
1,515,436 - +0.2
1,515,437 + +0.8
1,515,462 + +0.2
1,515,470 - -1.6
1,515,470 - -1.3
1,515,489 - -1.3
1,515,498 + -0.7
1,515,498 + -0.1
1,515,524 + -2.1
1,515,550 + -1.3
1,515,699 + MMJJ_RS08230 0.25 -2.1
1,515,700 - MMJJ_RS08230 0.25 -2.9
1,515,715 - MMJJ_RS08230 0.27 -2.0
1,515,716 + MMJJ_RS08230 0.28 +0.2
1,515,802 + MMJJ_RS08230 0.39 -1.8
1,515,986 - MMJJ_RS08230 0.65 +0.0
1,516,181 + -1.0
1,516,225 + -3.0
1,516,250 - +0.6
1,516,254 - +0.5
1,516,283 - -0.0
1,516,283 - +0.2
1,516,283 - +0.1
1,516,284 + +0.6
1,516,284 + +0.1
1,516,296 + +0.4
1,516,317 - -1.8
1,516,317 - -0.8
1,516,318 + -0.6
1,516,318 + +0.3
1,516,327 - +0.0
1,516,328 + -0.2
1,516,328 + -0.2
1,516,328 + -0.2
1,516,350 - -0.1
1,516,354 - -0.8
1,516,355 + +0.4
1,516,458 + MMJJ_RS08235 0.28 +0.8
1,516,511 + MMJJ_RS08235 0.47 +0.0
1,516,511 + MMJJ_RS08235 0.47 -3.0
1,516,590 + MMJJ_RS08235 0.76 -0.6
1,516,653 - +0.7
1,516,657 - -0.5
1,516,672 - +0.4
1,516,714 - +0.3
1,516,715 + -1.7
1,516,722 - +0.5
1,516,754 + -0.1
1,516,756 - +0.8
1,516,756 - -0.1
1,516,757 + -0.1
1,516,758 - -0.5
1,516,759 + +0.0
1,516,759 + +1.3
1,516,764 + +0.3
1,516,782 + -0.1
1,516,782 + +0.3
1,516,800 - MMJJ_RS08240 0.12 -0.8
1,516,800 - MMJJ_RS08240 0.12 -0.7
1,516,801 + MMJJ_RS08240 0.12 -0.2
1,516,812 + MMJJ_RS08240 0.14 +1.3
1,516,813 - MMJJ_RS08240 0.14 -0.9
1,516,829 + MMJJ_RS08240 0.17 +0.5
1,516,853 + MMJJ_RS08240 0.22 +0.6
1,516,915 + MMJJ_RS08240 0.33 -1.4
1,516,915 + MMJJ_RS08240 0.33 +0.2
1,516,923 - MMJJ_RS08240 0.34 +1.4
1,516,923 - MMJJ_RS08240 0.34 -0.1
1,516,924 + MMJJ_RS08240 0.35 -0.2
1,516,924 + MMJJ_RS08240 0.35 -0.1
1,516,924 + MMJJ_RS08240 0.35 +1.0
1,516,924 + MMJJ_RS08240 0.35 +0.6
1,516,924 + MMJJ_RS08240 0.35 +0.0
1,516,924 + MMJJ_RS08240 0.35 -0.4
1,516,928 - MMJJ_RS08240 0.35 +0.3
1,516,970 - MMJJ_RS08240 0.43 -1.1
1,517,004 - MMJJ_RS08240 0.49 +0.3
1,517,004 - MMJJ_RS08240 0.49 +0.1
1,517,005 + MMJJ_RS08240 0.50 +0.1
1,517,024 - MMJJ_RS08240 0.53 +0.5
1,517,024 - MMJJ_RS08240 0.53 +0.8
1,517,052 - MMJJ_RS08240 0.58 +0.6
1,517,054 - MMJJ_RS08240 0.59 +1.6
1,517,072 - MMJJ_RS08240 0.62 +0.6
1,517,073 + MMJJ_RS08240 0.62 +0.4
1,517,083 - MMJJ_RS08240 0.64 -1.0
1,517,097 + MMJJ_RS08240 0.66 -0.9
1,517,156 - MMJJ_RS08240 0.77 -0.1
1,517,170 - MMJJ_RS08240 0.80 -0.5
1,517,171 + MMJJ_RS08240 0.80 +0.7
1,517,171 + MMJJ_RS08240 0.80 -0.1
1,517,173 - MMJJ_RS08240 0.80 +0.2
1,517,173 - MMJJ_RS08240 0.80 +0.4
1,517,173 - MMJJ_RS08240 0.80 +0.1
1,517,175 - MMJJ_RS08240 0.81 +0.8
1,517,175 - MMJJ_RS08240 0.81 -0.9
1,517,175 - MMJJ_RS08240 0.81 +0.5
1,517,175 - MMJJ_RS08240 0.81 -1.0
1,517,176 + MMJJ_RS08240 0.81 -0.1
1,517,176 + MMJJ_RS08240 0.81 -0.1
1,517,176 + MMJJ_RS08240 0.81 -0.4
1,517,176 + MMJJ_RS08240 0.81 +1.5
1,517,176 + MMJJ_RS08240 0.81 +0.2
1,517,197 - MMJJ_RS08240 0.85 +0.7
1,517,201 - MMJJ_RS08240 0.86 +0.0
1,517,201 - MMJJ_RS08240 0.86 -0.9
1,517,202 + MMJJ_RS08240 0.86 -0.0
1,517,226 - +0.5
1,517,226 - -0.4
1,517,238 - -0.1
1,517,238 - +1.2
1,517,240 - -0.0
1,517,240 - +0.8
1,517,244 + -0.1
1,517,244 + -0.9
1,517,244 + +0.9
1,517,259 + +0.6
1,517,304 + +0.1
1,517,304 + -2.3
1,517,304 + -0.6
1,517,310 - +0.1
1,517,310 - -0.6
1,517,311 + +0.5
1,517,316 + +0.9
1,517,316 + -0.7
1,517,316 + -1.0
1,517,332 + +0.5

Or see this region's nucleotide sequence