Experiment: formate McAA B12 dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05645 and MMJJ_RS05650 are separated by 29 nucleotides MMJJ_RS05650 and MMJJ_RS05655 are separated by 209 nucleotides MMJJ_RS05655 and MMJJ_RS05660 are separated by 1 nucleotides
MMJJ_RS05645: MMJJ_RS05645 - 4Fe-4S binding protein, at 1,052,927 to 1,054,114
_RS05645
MMJJ_RS05650: MMJJ_RS05650 - formylmethanofuran dehydrogenase subunit B, at 1,054,144 to 1,055,448
_RS05650
MMJJ_RS05655: MMJJ_RS05655 - HisA/HisF family protein, at 1,055,658 to 1,056,326
_RS05655
MMJJ_RS05660: MMJJ_RS05660 - sulfopyruvate decarboxylase subunit beta, at 1,056,328 to 1,056,885
_RS05660
Position (kb)
1054
1055
1056 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1053.282 kb on - strand, within MMJJ_RS05645 at 1053.387 kb on - strand at 1053.408 kb on - strand, within MMJJ_RS05645 at 1053.409 kb on + strand, within MMJJ_RS05645 at 1053.410 kb on - strand, within MMJJ_RS05645 at 1053.410 kb on - strand, within MMJJ_RS05645 at 1053.410 kb on - strand, within MMJJ_RS05645 at 1053.410 kb on - strand, within MMJJ_RS05645 at 1053.420 kb on - strand, within MMJJ_RS05645 at 1053.781 kb on + strand at 1053.850 kb on - strand, within MMJJ_RS05645 at 1053.851 kb on + strand at 1053.988 kb on + strand, within MMJJ_RS05645 at 1053.988 kb on + strand at 1053.988 kb on + strand at 1054.037 kb on + strand at 1054.067 kb on + strand at 1054.166 kb on - strand at 1054.832 kb on - strand, within MMJJ_RS05650 at 1054.988 kb on + strand at 1054.989 kb on - strand, within MMJJ_RS05650 at 1054.992 kb on - strand, within MMJJ_RS05650 at 1055.023 kb on - strand at 1055.024 kb on + strand, within MMJJ_RS05650 at 1055.139 kb on + strand, within MMJJ_RS05650 at 1055.240 kb on - strand, within MMJJ_RS05650 at 1055.240 kb on - strand, within MMJJ_RS05650 at 1055.241 kb on + strand, within MMJJ_RS05650 at 1055.570 kb on - strand at 1055.570 kb on - strand at 1055.571 kb on + strand at 1055.576 kb on + strand at 1055.598 kb on - strand at 1055.600 kb on - strand at 1055.602 kb on - strand at 1055.603 kb on + strand at 1055.608 kb on + strand at 1055.634 kb on - strand at 1055.639 kb on + strand at 1055.639 kb on + strand at 1055.684 kb on + strand at 1055.684 kb on + strand at 1055.731 kb on + strand, within MMJJ_RS05655 at 1055.750 kb on + strand, within MMJJ_RS05655 at 1055.753 kb on + strand, within MMJJ_RS05655 at 1055.779 kb on - strand, within MMJJ_RS05655 at 1056.058 kb on - strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.059 kb on + strand, within MMJJ_RS05655 at 1056.070 kb on - strand, within MMJJ_RS05655 at 1056.161 kb on - strand, within MMJJ_RS05655 at 1056.161 kb on - strand, within MMJJ_RS05655 at 1056.162 kb on + strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.189 kb on - strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.190 kb on + strand, within MMJJ_RS05655 at 1056.206 kb on - strand, within MMJJ_RS05655 at 1056.270 kb on + strand at 1056.270 kb on + strand at 1056.275 kb on + strand at 1056.275 kb on + strand at 1056.324 kb on - strand at 1056.324 kb on - strand at 1056.325 kb on + strand at 1056.325 kb on + strand at 1056.328 kb on + strand at 1056.336 kb on - strand at 1056.337 kb on + strand at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.386 kb on - strand, within MMJJ_RS05660 at 1056.426 kb on - strand, within MMJJ_RS05660
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA B12 dropout remove 1,053,282 - MMJJ_RS05645 0.30 -0.9 1,053,387 - -1.4 1,053,408 - MMJJ_RS05645 0.40 +0.7 1,053,409 + MMJJ_RS05645 0.41 -2.4 1,053,410 - MMJJ_RS05645 0.41 -3.5 1,053,410 - MMJJ_RS05645 0.41 -0.6 1,053,410 - MMJJ_RS05645 0.41 -0.3 1,053,410 - MMJJ_RS05645 0.41 -2.0 1,053,420 - MMJJ_RS05645 0.41 -1.9 1,053,781 + -0.0 1,053,850 - MMJJ_RS05645 0.78 -2.0 1,053,851 + -1.6 1,053,988 + MMJJ_RS05645 0.89 -2.7 1,053,988 + -0.6 1,053,988 + -1.4 1,054,037 + -3.0 1,054,067 + -1.2 1,054,166 - -1.4 1,054,832 - MMJJ_RS05650 0.53 -0.6 1,054,988 + -0.6 1,054,989 - MMJJ_RS05650 0.65 -2.9 1,054,992 - MMJJ_RS05650 0.65 -2.6 1,055,023 - -1.4 1,055,024 + MMJJ_RS05650 0.67 -2.0 1,055,139 + MMJJ_RS05650 0.76 -2.6 1,055,240 - MMJJ_RS05650 0.84 -2.0 1,055,240 - MMJJ_RS05650 0.84 -2.2 1,055,241 + MMJJ_RS05650 0.84 -1.9 1,055,570 - -0.4 1,055,570 - +0.3 1,055,571 + -0.3 1,055,576 + +0.6 1,055,598 - -0.3 1,055,600 - -0.3 1,055,602 - +0.2 1,055,603 + -0.6 1,055,608 + +1.0 1,055,634 - +1.0 1,055,639 + +0.6 1,055,639 + -0.4 1,055,684 + -1.9 1,055,684 + -2.9 1,055,731 + MMJJ_RS05655 0.11 -2.0 1,055,750 + MMJJ_RS05655 0.14 -3.5 1,055,753 + MMJJ_RS05655 0.14 -3.4 1,055,779 - MMJJ_RS05655 0.18 -2.5 1,056,058 - MMJJ_RS05655 0.60 -3.4 1,056,059 + MMJJ_RS05655 0.60 -1.9 1,056,059 + MMJJ_RS05655 0.60 -2.4 1,056,059 + MMJJ_RS05655 0.60 -1.4 1,056,070 - MMJJ_RS05655 0.62 -0.2 1,056,161 - MMJJ_RS05655 0.75 -4.1 1,056,161 - MMJJ_RS05655 0.75 -2.4 1,056,162 + MMJJ_RS05655 0.75 -1.6 1,056,189 - MMJJ_RS05655 0.79 -3.4 1,056,189 - MMJJ_RS05655 0.79 -2.3 1,056,189 - MMJJ_RS05655 0.79 -2.0 1,056,189 - MMJJ_RS05655 0.79 -1.9 1,056,189 - MMJJ_RS05655 0.79 -0.9 1,056,190 + MMJJ_RS05655 0.80 -3.0 1,056,190 + MMJJ_RS05655 0.80 -2.0 1,056,190 + MMJJ_RS05655 0.80 -2.9 1,056,190 + MMJJ_RS05655 0.80 -2.7 1,056,206 - MMJJ_RS05655 0.82 -3.3 1,056,270 + -2.9 1,056,270 + -2.8 1,056,275 + -1.8 1,056,275 + -2.7 1,056,324 - +0.6 1,056,324 - +0.4 1,056,325 + -0.0 1,056,325 + +0.1 1,056,328 + +0.9 1,056,336 - +0.0 1,056,337 + +1.6 1,056,386 - MMJJ_RS05660 0.10 +1.4 1,056,386 - MMJJ_RS05660 0.10 -0.0 1,056,386 - MMJJ_RS05660 0.10 -0.1 1,056,426 - MMJJ_RS05660 0.18 +1.4
Or see this region's nucleotide sequence