Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_06475

Experiment: nitrate 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_06470 and AO353_06475 are separated by 31 nucleotidesAO353_06475 and AO353_06480 are separated by 12 nucleotidesAO353_06480 and AO353_06485 are separated by 9 nucleotides AO353_06470: AO353_06470 - rod shape-determining protein RodA, at 1,441,340 to 1,442,443 _06470 AO353_06475: AO353_06475 - penicillin-binding protein 2, at 1,442,475 to 1,444,370 _06475 AO353_06480: AO353_06480 - 50S rRNA methyltransferase, at 1,444,383 to 1,444,850 _06480 AO353_06485: AO353_06485 - ribosome silencing factor, at 1,444,860 to 1,445,354 _06485 Position (kb) 1442 1443 1444 1445Strain fitness (log2 ratio) -3 -2 -1 0 1at 1442.508 kb on - strandat 1442.518 kb on + strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.385 kb on - strandat 1444.394 kb on - strandat 1444.427 kb on - strandat 1444.427 kb on - strandat 1444.427 kb on - strandat 1444.427 kb on - strandat 1444.784 kb on - strand, within AO353_06480at 1444.784 kb on - strand, within AO353_06480at 1444.784 kb on - strand, within AO353_06480at 1444.784 kb on - strand, within AO353_06480at 1444.784 kb on - strand, within AO353_06480at 1444.784 kb on - strand, within AO353_06480at 1445.329 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 200 mM
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1,442,508 - +0.7
1,442,518 + -2.5
1,444,385 - +0.3
1,444,385 - +0.0
1,444,385 - -1.4
1,444,385 - -0.7
1,444,385 - -0.6
1,444,385 - -1.0
1,444,385 - -2.0
1,444,385 - -0.7
1,444,385 - -2.3
1,444,385 - -3.5
1,444,394 - -0.6
1,444,427 - -1.4
1,444,427 - +0.5
1,444,427 - -1.5
1,444,427 - -0.3
1,444,784 - AO353_06480 0.86 -1.1
1,444,784 - AO353_06480 0.86 -1.1
1,444,784 - AO353_06480 0.86 +0.6
1,444,784 - AO353_06480 0.86 -1.3
1,444,784 - AO353_06480 0.86 +0.7
1,444,784 - AO353_06480 0.86 +0.5
1,445,329 - -2.0

Or see this region's nucleotide sequence