Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_06470 and AO353_06475 are separated by 31 nucleotides AO353_06475 and AO353_06480 are separated by 12 nucleotides AO353_06480 and AO353_06485 are separated by 9 nucleotides
AO353_06470: AO353_06470 - rod shape-determining protein RodA, at 1,441,340 to 1,442,443
_06470
AO353_06475: AO353_06475 - penicillin-binding protein 2, at 1,442,475 to 1,444,370
_06475
AO353_06480: AO353_06480 - 50S rRNA methyltransferase, at 1,444,383 to 1,444,850
_06480
AO353_06485: AO353_06485 - ribosome silencing factor, at 1,444,860 to 1,445,354
_06485
Position (kb)
1442
1443
1444
1445 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1442.508 kb on - strand at 1442.518 kb on + strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.385 kb on - strand at 1444.394 kb on - strand at 1444.427 kb on - strand at 1444.427 kb on - strand at 1444.427 kb on - strand at 1444.427 kb on - strand at 1444.784 kb on - strand, within AO353_06480 at 1444.784 kb on - strand, within AO353_06480 at 1444.784 kb on - strand, within AO353_06480 at 1444.784 kb on - strand, within AO353_06480 at 1444.784 kb on - strand, within AO353_06480 at 1444.784 kb on - strand, within AO353_06480 at 1445.329 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 1,442,508 - +0.7 1,442,518 + -2.5 1,444,385 - +0.3 1,444,385 - +0.0 1,444,385 - -1.4 1,444,385 - -0.7 1,444,385 - -0.6 1,444,385 - -1.0 1,444,385 - -2.0 1,444,385 - -0.7 1,444,385 - -2.3 1,444,385 - -3.5 1,444,394 - -0.6 1,444,427 - -1.4 1,444,427 - +0.5 1,444,427 - -1.5 1,444,427 - -0.3 1,444,784 - AO353_06480 0.86 -1.1 1,444,784 - AO353_06480 0.86 -1.1 1,444,784 - AO353_06480 0.86 +0.6 1,444,784 - AO353_06480 0.86 -1.3 1,444,784 - AO353_06480 0.86 +0.7 1,444,784 - AO353_06480 0.86 +0.5 1,445,329 - -2.0
Or see this region's nucleotide sequence