Experiment: Amylose from potato
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0280 and BT0281 are separated by 249 nucleotides BT0281 and BT0282 are separated by 301 nucleotides
BT0280: BT0280 - transposase for insertion sequence element ISRM3 (NCBI ptt file), at 324,541 to 325,758
BT0280
BT0281: BT0281 - putative DNA-binding protein (NCBI ptt file), at 326,008 to 326,649
BT0281
BT0282: BT0282 - conserved hypothetical protein (NCBI ptt file), at 326,951 to 328,246
BT0282
Position (kb) 326
327 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 326.039 kb on - strand at 326.066 kb on + strand at 326.067 kb on - strand at 326.068 kb on + strand at 326.208 kb on - strand, within BT0281 at 326.314 kb on - strand, within BT0281 at 326.323 kb on - strand, within BT0281 at 326.330 kb on - strand, within BT0281 at 326.330 kb on - strand, within BT0281 at 326.331 kb on + strand, within BT0281 at 326.332 kb on - strand, within BT0281 at 326.417 kb on - strand, within BT0281 at 326.626 kb on - strand at 326.627 kb on + strand at 326.849 kb on - strand at 326.917 kb on - strand at 327.046 kb on - strand at 327.061 kb on + strand at 327.062 kb on - strand at 327.461 kb on - strand, within BT0282 at 327.476 kb on - strand, within BT0282 at 327.555 kb on - strand, within BT0282 at 327.558 kb on + strand, within BT0282 at 327.559 kb on - strand, within BT0282 at 327.646 kb on - strand, within BT0282
Per-strain Table
Position Strand Gene LocusTag Fraction Amylose from potato remove 326,039 - -1.9 326,066 + -4.2 326,067 - -0.8 326,068 + -0.8 326,208 - BT0281 0.31 -2.2 326,314 - BT0281 0.48 -1.6 326,323 - BT0281 0.49 -1.3 326,330 - BT0281 0.50 -2.5 326,330 - BT0281 0.50 -0.9 326,331 + BT0281 0.50 +1.0 326,332 - BT0281 0.50 -1.5 326,417 - BT0281 0.64 -2.5 326,626 - -0.5 326,627 + -0.6 326,849 - -1.5 326,917 - -1.6 327,046 - +0.5 327,061 + -1.2 327,062 - -0.5 327,461 - BT0282 0.39 -1.6 327,476 - BT0282 0.41 -0.4 327,555 - BT0282 0.47 -1.3 327,558 + BT0282 0.47 -1.1 327,559 - BT0282 0.47 +0.4 327,646 - BT0282 0.54 -0.3
Or see this region's nucleotide sequence