Experiment: formate McAA B12 dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03680 and MMJJ_RS03685 are separated by 367 nucleotides MMJJ_RS03685 and MMJJ_RS03690 overlap by 4 nucleotides MMJJ_RS03690 and MMJJ_RS03695 overlap by 8 nucleotides
MMJJ_RS03680: MMJJ_RS03680 - fructose-1,6-bisphosphatase, at 687,541 to 688,692
_RS03680
MMJJ_RS03685: MMJJ_RS03685 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 689,060 to 690,898
_RS03685
MMJJ_RS03690: MMJJ_RS03690 - indolepyruvate oxidoreductase subunit beta, at 690,895 to 691,494
_RS03690
MMJJ_RS03695: MMJJ_RS03695 - phenylacetate--CoA ligase, at 691,487 to 692,731
_RS03695
Position (kb)
689
690
691 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 688.222 kb on - strand, within MMJJ_RS03680 at 688.262 kb on - strand at 688.731 kb on - strand at 688.736 kb on - strand at 688.791 kb on - strand at 688.812 kb on - strand at 688.813 kb on + strand at 688.813 kb on + strand at 688.816 kb on - strand at 688.824 kb on - strand at 688.831 kb on - strand at 688.832 kb on + strand at 688.832 kb on + strand at 688.833 kb on - strand at 688.833 kb on - strand at 688.834 kb on + strand at 688.835 kb on - strand at 688.835 kb on - strand at 688.835 kb on - strand at 688.835 kb on - strand at 688.835 kb on - strand at 688.835 kb on - strand at 688.835 kb on - strand at 688.836 kb on + strand at 688.836 kb on + strand at 688.836 kb on + strand at 688.839 kb on - strand at 688.858 kb on - strand at 688.859 kb on + strand at 688.862 kb on - strand at 688.862 kb on - strand at 688.863 kb on + strand at 688.867 kb on + strand at 688.867 kb on + strand at 688.871 kb on - strand at 688.871 kb on - strand at 688.871 kb on - strand at 688.872 kb on + strand at 688.872 kb on + strand at 688.872 kb on + strand at 688.872 kb on + strand at 688.872 kb on + strand at 688.874 kb on - strand at 688.875 kb on + strand at 688.881 kb on - strand at 688.882 kb on + strand at 688.886 kb on - strand at 688.897 kb on - strand at 688.897 kb on - strand at 688.908 kb on - strand at 688.908 kb on - strand at 688.937 kb on + strand at 688.958 kb on + strand at 688.973 kb on - strand at 688.974 kb on + strand at 688.977 kb on - strand at 688.978 kb on + strand at 688.978 kb on + strand at 688.979 kb on - strand at 688.980 kb on + strand at 688.980 kb on + strand at 688.982 kb on - strand at 688.982 kb on - strand at 688.982 kb on - strand at 688.982 kb on - strand at 688.982 kb on - strand at 688.983 kb on + strand at 688.983 kb on + strand at 688.983 kb on + strand at 688.984 kb on - strand at 688.985 kb on + strand at 688.985 kb on + strand at 688.986 kb on - strand at 688.987 kb on + strand at 689.044 kb on - strand at 689.044 kb on - strand at 689.044 kb on - strand at 689.044 kb on - strand at 689.047 kb on - strand at 689.065 kb on - strand at 689.068 kb on - strand at 689.102 kb on - strand at 689.178 kb on - strand at 689.201 kb on + strand at 689.204 kb on - strand at 689.205 kb on + strand at 689.207 kb on - strand at 689.208 kb on + strand at 689.208 kb on + strand at 689.208 kb on + strand at 689.287 kb on + strand, within MMJJ_RS03685 at 689.290 kb on - strand, within MMJJ_RS03685 at 689.337 kb on - strand, within MMJJ_RS03685 at 689.342 kb on - strand, within MMJJ_RS03685 at 689.343 kb on + strand, within MMJJ_RS03685 at 689.523 kb on + strand, within MMJJ_RS03685 at 689.535 kb on - strand, within MMJJ_RS03685 at 689.548 kb on - strand, within MMJJ_RS03685 at 689.548 kb on - strand, within MMJJ_RS03685 at 689.549 kb on + strand, within MMJJ_RS03685 at 689.549 kb on + strand, within MMJJ_RS03685 at 689.580 kb on - strand, within MMJJ_RS03685 at 689.580 kb on - strand, within MMJJ_RS03685 at 689.580 kb on - strand, within MMJJ_RS03685 at 689.580 kb on - strand, within MMJJ_RS03685 at 689.581 kb on + strand, within MMJJ_RS03685 at 689.583 kb on - strand, within MMJJ_RS03685 at 689.583 kb on - strand, within MMJJ_RS03685 at 689.583 kb on - strand, within MMJJ_RS03685 at 689.584 kb on + strand, within MMJJ_RS03685 at 689.595 kb on - strand, within MMJJ_RS03685 at 689.595 kb on - strand, within MMJJ_RS03685 at 689.595 kb on - strand, within MMJJ_RS03685 at 689.595 kb on - strand, within MMJJ_RS03685 at 689.596 kb on + strand, within MMJJ_RS03685 at 689.615 kb on + strand, within MMJJ_RS03685 at 689.617 kb on + strand, within MMJJ_RS03685 at 689.617 kb on + strand, within MMJJ_RS03685 at 689.617 kb on + strand, within MMJJ_RS03685 at 689.706 kb on - strand, within MMJJ_RS03685 at 689.787 kb on + strand, within MMJJ_RS03685 at 689.787 kb on + strand, within MMJJ_RS03685 at 689.796 kb on - strand, within MMJJ_RS03685 at 689.914 kb on + strand, within MMJJ_RS03685 at 689.938 kb on + strand, within MMJJ_RS03685 at 689.949 kb on - strand, within MMJJ_RS03685 at 690.036 kb on - strand, within MMJJ_RS03685 at 690.037 kb on + strand, within MMJJ_RS03685 at 690.066 kb on - strand, within MMJJ_RS03685 at 690.078 kb on - strand, within MMJJ_RS03685 at 690.119 kb on + strand, within MMJJ_RS03685 at 690.129 kb on - strand, within MMJJ_RS03685 at 690.191 kb on - strand, within MMJJ_RS03685 at 690.256 kb on - strand, within MMJJ_RS03685 at 690.266 kb on - strand, within MMJJ_RS03685 at 690.267 kb on + strand, within MMJJ_RS03685 at 690.292 kb on + strand, within MMJJ_RS03685 at 690.292 kb on + strand, within MMJJ_RS03685 at 690.304 kb on - strand, within MMJJ_RS03685 at 690.305 kb on + strand, within MMJJ_RS03685 at 690.331 kb on - strand, within MMJJ_RS03685 at 690.332 kb on + strand, within MMJJ_RS03685 at 690.385 kb on - strand, within MMJJ_RS03685 at 690.385 kb on - strand, within MMJJ_RS03685 at 690.426 kb on - strand, within MMJJ_RS03685 at 690.426 kb on - strand, within MMJJ_RS03685 at 690.427 kb on + strand, within MMJJ_RS03685 at 690.430 kb on - strand, within MMJJ_RS03685 at 690.431 kb on + strand, within MMJJ_RS03685 at 690.441 kb on + strand, within MMJJ_RS03685 at 690.457 kb on - strand, within MMJJ_RS03685 at 690.457 kb on - strand, within MMJJ_RS03685 at 690.544 kb on - strand, within MMJJ_RS03685 at 690.545 kb on + strand, within MMJJ_RS03685 at 690.545 kb on + strand, within MMJJ_RS03685 at 690.631 kb on - strand, within MMJJ_RS03685 at 690.631 kb on - strand, within MMJJ_RS03685 at 690.631 kb on - strand, within MMJJ_RS03685 at 690.653 kb on + strand, within MMJJ_RS03685 at 690.653 kb on + strand, within MMJJ_RS03685 at 690.659 kb on - strand, within MMJJ_RS03685 at 690.661 kb on - strand, within MMJJ_RS03685 at 690.749 kb on + strand at 690.749 kb on + strand at 690.751 kb on + strand at 690.796 kb on - strand at 690.796 kb on - strand at 690.796 kb on - strand at 690.796 kb on - strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.876 kb on - strand at 690.908 kb on - strand at 690.912 kb on + strand at 690.912 kb on + strand at 690.996 kb on - strand, within MMJJ_RS03690 at 691.040 kb on - strand, within MMJJ_RS03690 at 691.107 kb on + strand, within MMJJ_RS03690 at 691.110 kb on + strand, within MMJJ_RS03690 at 691.222 kb on - strand, within MMJJ_RS03690 at 691.223 kb on + strand, within MMJJ_RS03690 at 691.254 kb on - strand, within MMJJ_RS03690 at 691.255 kb on + strand, within MMJJ_RS03690 at 691.258 kb on - strand, within MMJJ_RS03690 at 691.258 kb on - strand, within MMJJ_RS03690 at 691.258 kb on - strand, within MMJJ_RS03690 at 691.259 kb on + strand, within MMJJ_RS03690 at 691.259 kb on + strand, within MMJJ_RS03690 at 691.354 kb on - strand, within MMJJ_RS03690 at 691.379 kb on + strand, within MMJJ_RS03690 at 691.379 kb on + strand, within MMJJ_RS03690 at 691.432 kb on + strand, within MMJJ_RS03690 at 691.492 kb on - strand at 691.495 kb on - strand at 691.511 kb on - strand at 691.590 kb on - strand at 691.591 kb on + strand at 691.656 kb on - strand, within MMJJ_RS03695 at 691.713 kb on - strand, within MMJJ_RS03695 at 691.757 kb on - strand, within MMJJ_RS03695 at 691.758 kb on + strand, within MMJJ_RS03695 at 691.805 kb on - strand, within MMJJ_RS03695 at 691.806 kb on + strand, within MMJJ_RS03695 at 691.834 kb on - strand at 691.835 kb on + strand, within MMJJ_RS03695 at 691.839 kb on - strand, within MMJJ_RS03695 at 691.891 kb on + strand, within MMJJ_RS03695
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA B12 dropout remove 688,222 - MMJJ_RS03680 0.59 -1.8 688,262 - +0.8 688,731 - -0.8 688,736 - -1.5 688,791 - +1.8 688,812 - +1.0 688,813 + +1.8 688,813 + +0.2 688,816 - -0.9 688,824 - -0.1 688,831 - +0.7 688,832 + -2.2 688,832 + -2.2 688,833 - +1.8 688,833 - +1.5 688,834 + +1.6 688,835 - -2.2 688,835 - +1.2 688,835 - +0.8 688,835 - +0.7 688,835 - -0.2 688,835 - +0.2 688,835 - -0.7 688,836 + -0.2 688,836 + -0.5 688,836 + -0.9 688,839 - -0.6 688,858 - -0.2 688,859 + +0.2 688,862 - -1.1 688,862 - +0.2 688,863 + +0.8 688,867 + +0.4 688,867 + +0.8 688,871 - -0.1 688,871 - -0.4 688,871 - +0.5 688,872 + -0.5 688,872 + +0.1 688,872 + +0.5 688,872 + +0.5 688,872 + +1.0 688,874 - -0.1 688,875 + +1.4 688,881 - +0.6 688,882 + +1.0 688,886 - +0.0 688,897 - -0.4 688,897 - -1.3 688,908 - -0.8 688,908 - +0.3 688,937 + -0.1 688,958 + -1.2 688,973 - -0.1 688,974 + +0.1 688,977 - -0.5 688,978 + +0.1 688,978 + +0.8 688,979 - -0.1 688,980 + -0.6 688,980 + +0.2 688,982 - -1.1 688,982 - -0.5 688,982 - +0.4 688,982 - -0.6 688,982 - +0.2 688,983 + -3.8 688,983 + -0.5 688,983 + +0.1 688,984 - +0.8 688,985 + -0.9 688,985 + -0.8 688,986 - -0.2 688,987 + +0.3 689,044 - -0.7 689,044 - -1.3 689,044 - -0.2 689,044 - +0.1 689,047 - +0.5 689,065 - +0.9 689,068 - -0.9 689,102 - +0.5 689,178 - +0.1 689,201 + -0.1 689,204 - -0.6 689,205 + -0.2 689,207 - -0.2 689,208 + +0.8 689,208 + +0.3 689,208 + +0.8 689,287 + MMJJ_RS03685 0.12 +1.4 689,290 - MMJJ_RS03685 0.13 +0.1 689,337 - MMJJ_RS03685 0.15 -0.1 689,342 - MMJJ_RS03685 0.15 +0.4 689,343 + MMJJ_RS03685 0.15 -0.6 689,523 + MMJJ_RS03685 0.25 -0.8 689,535 - MMJJ_RS03685 0.26 +0.5 689,548 - MMJJ_RS03685 0.27 +0.5 689,548 - MMJJ_RS03685 0.27 +0.8 689,549 + MMJJ_RS03685 0.27 +0.1 689,549 + MMJJ_RS03685 0.27 +1.0 689,580 - MMJJ_RS03685 0.28 +1.3 689,580 - MMJJ_RS03685 0.28 +1.3 689,580 - MMJJ_RS03685 0.28 +0.9 689,580 - MMJJ_RS03685 0.28 +0.1 689,581 + MMJJ_RS03685 0.28 -0.3 689,583 - MMJJ_RS03685 0.28 -0.1 689,583 - MMJJ_RS03685 0.28 -0.5 689,583 - MMJJ_RS03685 0.28 -0.3 689,584 + MMJJ_RS03685 0.28 +0.5 689,595 - MMJJ_RS03685 0.29 -1.7 689,595 - MMJJ_RS03685 0.29 +0.6 689,595 - MMJJ_RS03685 0.29 +0.9 689,595 - MMJJ_RS03685 0.29 -0.6 689,596 + MMJJ_RS03685 0.29 +1.3 689,615 + MMJJ_RS03685 0.30 +0.3 689,617 + MMJJ_RS03685 0.30 +0.5 689,617 + MMJJ_RS03685 0.30 +0.1 689,617 + MMJJ_RS03685 0.30 -0.5 689,706 - MMJJ_RS03685 0.35 -2.2 689,787 + MMJJ_RS03685 0.40 +0.7 689,787 + MMJJ_RS03685 0.40 +0.4 689,796 - MMJJ_RS03685 0.40 +0.3 689,914 + MMJJ_RS03685 0.46 +0.9 689,938 + MMJJ_RS03685 0.48 -0.4 689,949 - MMJJ_RS03685 0.48 -0.7 690,036 - MMJJ_RS03685 0.53 +0.9 690,037 + MMJJ_RS03685 0.53 -0.9 690,066 - MMJJ_RS03685 0.55 +0.8 690,078 - MMJJ_RS03685 0.55 -0.4 690,119 + MMJJ_RS03685 0.58 +0.4 690,129 - MMJJ_RS03685 0.58 -1.0 690,191 - MMJJ_RS03685 0.62 +0.3 690,256 - MMJJ_RS03685 0.65 -0.8 690,266 - MMJJ_RS03685 0.66 +1.2 690,267 + MMJJ_RS03685 0.66 -0.5 690,292 + MMJJ_RS03685 0.67 +1.1 690,292 + MMJJ_RS03685 0.67 +0.1 690,304 - MMJJ_RS03685 0.68 +1.2 690,305 + MMJJ_RS03685 0.68 -1.5 690,331 - MMJJ_RS03685 0.69 -2.0 690,332 + MMJJ_RS03685 0.69 +0.3 690,385 - MMJJ_RS03685 0.72 -1.8 690,385 - MMJJ_RS03685 0.72 -0.2 690,426 - MMJJ_RS03685 0.74 -1.0 690,426 - MMJJ_RS03685 0.74 +0.6 690,427 + MMJJ_RS03685 0.74 +0.3 690,430 - MMJJ_RS03685 0.74 -0.7 690,431 + MMJJ_RS03685 0.75 -0.5 690,441 + MMJJ_RS03685 0.75 -0.3 690,457 - MMJJ_RS03685 0.76 -0.1 690,457 - MMJJ_RS03685 0.76 -0.1 690,544 - MMJJ_RS03685 0.81 +1.5 690,545 + MMJJ_RS03685 0.81 +0.3 690,545 + MMJJ_RS03685 0.81 +0.2 690,631 - MMJJ_RS03685 0.85 -0.1 690,631 - MMJJ_RS03685 0.85 +0.8 690,631 - MMJJ_RS03685 0.85 +1.1 690,653 + MMJJ_RS03685 0.87 -0.3 690,653 + MMJJ_RS03685 0.87 +0.7 690,659 - MMJJ_RS03685 0.87 +1.0 690,661 - MMJJ_RS03685 0.87 +0.5 690,749 + -0.8 690,749 + -1.5 690,751 + -0.1 690,796 - +0.6 690,796 - +0.7 690,796 - +0.1 690,796 - +0.9 690,797 + -1.3 690,797 + +0.3 690,797 + -0.9 690,797 + +0.4 690,797 + -2.2 690,797 + +0.3 690,876 - -0.9 690,908 - +0.3 690,912 + +1.4 690,912 + -0.0 690,996 - MMJJ_RS03690 0.17 -0.1 691,040 - MMJJ_RS03690 0.24 -0.8 691,107 + MMJJ_RS03690 0.35 -1.5 691,110 + MMJJ_RS03690 0.36 -2.0 691,222 - MMJJ_RS03690 0.55 +0.6 691,223 + MMJJ_RS03690 0.55 -1.2 691,254 - MMJJ_RS03690 0.60 -0.3 691,255 + MMJJ_RS03690 0.60 +0.0 691,258 - MMJJ_RS03690 0.60 -0.3 691,258 - MMJJ_RS03690 0.60 +0.7 691,258 - MMJJ_RS03690 0.60 +0.1 691,259 + MMJJ_RS03690 0.61 +0.5 691,259 + MMJJ_RS03690 0.61 -0.5 691,354 - MMJJ_RS03690 0.77 +0.3 691,379 + MMJJ_RS03690 0.81 -0.8 691,379 + MMJJ_RS03690 0.81 +0.6 691,432 + MMJJ_RS03690 0.90 -0.5 691,492 - +0.7 691,495 - -2.1 691,511 - +0.3 691,590 - -0.2 691,591 + +0.7 691,656 - MMJJ_RS03695 0.14 -0.1 691,713 - MMJJ_RS03695 0.18 +0.3 691,757 - MMJJ_RS03695 0.22 +0.6 691,758 + MMJJ_RS03695 0.22 -0.4 691,805 - MMJJ_RS03695 0.26 +0.4 691,806 + MMJJ_RS03695 0.26 +0.8 691,834 - -0.8 691,835 + MMJJ_RS03695 0.28 +0.7 691,839 - MMJJ_RS03695 0.28 +0.5 691,891 + MMJJ_RS03695 0.32 +0.4
Or see this region's nucleotide sequence