Experiment: formate McAA B12 dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03010 and MMJJ_RS03015 are separated by 83 nucleotides
MMJJ_RS03010: MMJJ_RS03010 - replication factor C small subunit, at 563,261 to 564,208
_RS03010
MMJJ_RS03015: MMJJ_RS03015 - Ig-like domain-containing protein, at 564,292 to 566,091
_RS03015
Position (kb)
564
565
566
567 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 563.826 kb on + strand, within MMJJ_RS03010 at 563.866 kb on + strand, within MMJJ_RS03010 at 564.083 kb on + strand, within MMJJ_RS03010 at 564.244 kb on - strand at 564.286 kb on + strand at 564.293 kb on - strand at 564.301 kb on - strand at 564.359 kb on + strand at 564.376 kb on - strand at 564.377 kb on + strand at 564.415 kb on + strand at 564.416 kb on - strand at 564.416 kb on - strand at 564.416 kb on - strand at 564.416 kb on - strand at 564.416 kb on - strand at 564.416 kb on - strand at 564.416 kb on - strand at 564.416 kb on - strand at 564.417 kb on + strand at 564.417 kb on + strand at 564.417 kb on + strand at 564.417 kb on + strand at 564.417 kb on + strand at 564.417 kb on + strand at 564.421 kb on - strand at 564.422 kb on + strand at 564.428 kb on - strand at 564.428 kb on - strand at 564.462 kb on - strand at 564.462 kb on - strand at 564.463 kb on + strand at 564.536 kb on - strand, within MMJJ_RS03015 at 564.536 kb on - strand, within MMJJ_RS03015 at 564.537 kb on + strand, within MMJJ_RS03015 at 564.537 kb on + strand, within MMJJ_RS03015 at 564.617 kb on + strand, within MMJJ_RS03015 at 564.617 kb on + strand, within MMJJ_RS03015 at 564.641 kb on - strand, within MMJJ_RS03015 at 564.667 kb on - strand, within MMJJ_RS03015 at 564.668 kb on + strand, within MMJJ_RS03015 at 564.696 kb on - strand, within MMJJ_RS03015 at 564.697 kb on + strand, within MMJJ_RS03015 at 564.711 kb on - strand, within MMJJ_RS03015 at 564.712 kb on + strand at 564.712 kb on + strand, within MMJJ_RS03015 at 564.771 kb on - strand, within MMJJ_RS03015 at 564.772 kb on + strand, within MMJJ_RS03015 at 564.772 kb on + strand, within MMJJ_RS03015 at 564.787 kb on - strand, within MMJJ_RS03015 at 564.788 kb on + strand, within MMJJ_RS03015 at 564.788 kb on + strand, within MMJJ_RS03015 at 564.788 kb on + strand, within MMJJ_RS03015 at 564.852 kb on + strand, within MMJJ_RS03015 at 564.852 kb on + strand, within MMJJ_RS03015 at 564.888 kb on + strand, within MMJJ_RS03015 at 564.890 kb on - strand, within MMJJ_RS03015 at 564.891 kb on + strand, within MMJJ_RS03015 at 564.921 kb on - strand, within MMJJ_RS03015 at 565.005 kb on - strand, within MMJJ_RS03015 at 565.005 kb on - strand, within MMJJ_RS03015 at 565.005 kb on - strand, within MMJJ_RS03015 at 565.005 kb on - strand, within MMJJ_RS03015 at 565.006 kb on + strand, within MMJJ_RS03015 at 565.007 kb on - strand, within MMJJ_RS03015 at 565.007 kb on - strand, within MMJJ_RS03015 at 565.007 kb on - strand, within MMJJ_RS03015 at 565.007 kb on - strand, within MMJJ_RS03015 at 565.008 kb on + strand, within MMJJ_RS03015 at 565.008 kb on + strand, within MMJJ_RS03015 at 565.038 kb on - strand, within MMJJ_RS03015 at 565.290 kb on - strand, within MMJJ_RS03015 at 565.290 kb on - strand, within MMJJ_RS03015 at 565.533 kb on + strand, within MMJJ_RS03015 at 565.533 kb on + strand, within MMJJ_RS03015 at 565.533 kb on + strand, within MMJJ_RS03015 at 565.539 kb on + strand, within MMJJ_RS03015 at 565.539 kb on + strand, within MMJJ_RS03015 at 565.576 kb on - strand, within MMJJ_RS03015 at 565.576 kb on - strand at 565.576 kb on - strand, within MMJJ_RS03015 at 565.590 kb on - strand, within MMJJ_RS03015 at 565.590 kb on - strand, within MMJJ_RS03015
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA B12 dropout remove 563,826 + MMJJ_RS03010 0.60 -1.7 563,866 + MMJJ_RS03010 0.64 -3.2 564,083 + MMJJ_RS03010 0.87 -0.7 564,244 - -0.8 564,286 + -0.3 564,293 - +0.5 564,301 - +0.4 564,359 + -0.2 564,376 - -0.1 564,377 + +1.2 564,415 + +0.4 564,416 - -0.0 564,416 - +0.1 564,416 - +0.4 564,416 - -0.3 564,416 - +0.6 564,416 - +0.7 564,416 - +0.0 564,416 - +0.9 564,417 + +0.9 564,417 + -0.7 564,417 + +0.2 564,417 + +0.2 564,417 + +0.2 564,417 + -0.4 564,421 - -0.7 564,422 + +0.1 564,428 - -0.9 564,428 - -0.4 564,462 - -3.5 564,462 - +0.3 564,463 + +0.2 564,536 - MMJJ_RS03015 0.14 +0.4 564,536 - MMJJ_RS03015 0.14 +1.4 564,537 + MMJJ_RS03015 0.14 +0.6 564,537 + MMJJ_RS03015 0.14 +0.3 564,617 + MMJJ_RS03015 0.18 -0.2 564,617 + MMJJ_RS03015 0.18 -0.1 564,641 - MMJJ_RS03015 0.19 -0.5 564,667 - MMJJ_RS03015 0.21 +0.3 564,668 + MMJJ_RS03015 0.21 +2.0 564,696 - MMJJ_RS03015 0.22 -0.2 564,697 + MMJJ_RS03015 0.23 +0.7 564,711 - MMJJ_RS03015 0.23 +0.6 564,712 + -0.2 564,712 + MMJJ_RS03015 0.23 +0.7 564,771 - MMJJ_RS03015 0.27 -0.2 564,772 + MMJJ_RS03015 0.27 +0.5 564,772 + MMJJ_RS03015 0.27 +0.1 564,787 - MMJJ_RS03015 0.28 -1.7 564,788 + MMJJ_RS03015 0.28 +0.8 564,788 + MMJJ_RS03015 0.28 -0.2 564,788 + MMJJ_RS03015 0.28 +0.1 564,852 + MMJJ_RS03015 0.31 -0.4 564,852 + MMJJ_RS03015 0.31 +0.6 564,888 + MMJJ_RS03015 0.33 +0.6 564,890 - MMJJ_RS03015 0.33 +0.7 564,891 + MMJJ_RS03015 0.33 +0.3 564,921 - MMJJ_RS03015 0.35 +0.5 565,005 - MMJJ_RS03015 0.40 +0.6 565,005 - MMJJ_RS03015 0.40 -0.2 565,005 - MMJJ_RS03015 0.40 -0.0 565,005 - MMJJ_RS03015 0.40 +0.1 565,006 + MMJJ_RS03015 0.40 -0.7 565,007 - MMJJ_RS03015 0.40 +0.6 565,007 - MMJJ_RS03015 0.40 +1.2 565,007 - MMJJ_RS03015 0.40 -0.3 565,007 - MMJJ_RS03015 0.40 +1.0 565,008 + MMJJ_RS03015 0.40 -1.0 565,008 + MMJJ_RS03015 0.40 -0.9 565,038 - MMJJ_RS03015 0.41 -1.2 565,290 - MMJJ_RS03015 0.55 +1.3 565,290 - MMJJ_RS03015 0.55 -0.0 565,533 + MMJJ_RS03015 0.69 -0.1 565,533 + MMJJ_RS03015 0.69 +0.6 565,533 + MMJJ_RS03015 0.69 -0.2 565,539 + MMJJ_RS03015 0.69 -1.5 565,539 + MMJJ_RS03015 0.69 -0.7 565,576 - MMJJ_RS03015 0.71 +1.0 565,576 - -0.7 565,576 - MMJJ_RS03015 0.71 +0.5 565,590 - MMJJ_RS03015 0.72 +0.9 565,590 - MMJJ_RS03015 0.72 -0.3
Or see this region's nucleotide sequence