Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26955 and BPHYT_RS26960 are separated by 155 nucleotides BPHYT_RS26960 and BPHYT_RS26965 are separated by 73 nucleotides BPHYT_RS26965 and BPHYT_RS26970 overlap by 14 nucleotides
BPHYT_RS26955: BPHYT_RS26955 - succinylglutamate desuccinylase, at 1,625,416 to 1,626,429
_RS26955
BPHYT_RS26960: BPHYT_RS26960 - LysR family transcriptional regulator, at 1,626,585 to 1,627,469
_RS26960
BPHYT_RS26965: BPHYT_RS26965 - L-lactate dehydrogenase, LldG subunit (from data) , at 1,627,543 to 1,628,130
_RS26965
BPHYT_RS26970: BPHYT_RS26970 - L-lactate dehydrogenase, LldF subunit (from data) , at 1,628,117 to 1,629,511
_RS26970
Position (kb)
1626
1627
1628 Strain fitness (log2 ratio)
-2
-1
0
1 at 1625.813 kb on - strand, within BPHYT_RS26955 at 1626.491 kb on - strand at 1626.491 kb on - strand at 1626.500 kb on - strand at 1626.554 kb on + strand at 1626.554 kb on + strand at 1626.856 kb on + strand, within BPHYT_RS26960 at 1627.279 kb on + strand, within BPHYT_RS26960 at 1627.769 kb on + strand, within BPHYT_RS26965 at 1628.212 kb on + strand at 1628.213 kb on - strand at 1628.225 kb on - strand at 1628.286 kb on + strand, within BPHYT_RS26970 at 1628.385 kb on + strand, within BPHYT_RS26970 at 1628.386 kb on - strand, within BPHYT_RS26970 at 1628.467 kb on + strand, within BPHYT_RS26970
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 1,625,813 - BPHYT_RS26955 0.39 +0.9 1,626,491 - -0.0 1,626,491 - -2.7 1,626,500 - +0.3 1,626,554 + +0.5 1,626,554 + +0.5 1,626,856 + BPHYT_RS26960 0.31 +0.4 1,627,279 + BPHYT_RS26960 0.78 -1.2 1,627,769 + BPHYT_RS26965 0.38 -1.2 1,628,212 + +1.4 1,628,213 - -2.2 1,628,225 - +0.0 1,628,286 + BPHYT_RS26970 0.12 +0.5 1,628,385 + BPHYT_RS26970 0.19 -0.4 1,628,386 - BPHYT_RS26970 0.19 -1.6 1,628,467 + BPHYT_RS26970 0.25 +0.7
Or see this region's nucleotide sequence