Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26380 and BPHYT_RS26385 are separated by 27 nucleotides BPHYT_RS26385 and BPHYT_RS26390 are separated by 87 nucleotides BPHYT_RS26390 and BPHYT_RS26395 are separated by 373 nucleotides BPHYT_RS26395 and BPHYT_RS26400 overlap by 4 nucleotides
BPHYT_RS26380: BPHYT_RS26380 - cobalamin biosynthesis protein CobT, at 1,491,243 to 1,492,991
_RS26380
BPHYT_RS26385: BPHYT_RS26385 - glutamine amidotransferase, at 1,493,019 to 1,493,852
_RS26385
BPHYT_RS26390: BPHYT_RS26390 - antibiotic resistance protein, at 1,493,940 to 1,494,617
_RS26390
BPHYT_RS26395: BPHYT_RS26395 - membrane protein, at 1,494,991 to 1,495,206
_RS26395
BPHYT_RS26400: BPHYT_RS26400 - sodium:solute symporter, at 1,495,203 to 1,496,675
_RS26400
Position (kb)
1493
1494
1495 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1493.498 kb on - strand, within BPHYT_RS26385 at 1493.853 kb on - strand at 1493.853 kb on - strand at 1494.182 kb on + strand, within BPHYT_RS26390 at 1494.617 kb on + strand at 1494.777 kb on + strand at 1494.857 kb on - strand at 1494.928 kb on + strand at 1494.964 kb on + strand at 1494.964 kb on + strand at 1495.450 kb on - strand, within BPHYT_RS26400
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 1,493,498 - BPHYT_RS26385 0.57 -0.3 1,493,853 - -0.1 1,493,853 - -3.3 1,494,182 + BPHYT_RS26390 0.36 -3.5 1,494,617 + +2.6 1,494,777 + +0.5 1,494,857 - -0.1 1,494,928 + +0.2 1,494,964 + -0.0 1,494,964 + +1.8 1,495,450 - BPHYT_RS26400 0.17 -0.0
Or see this region's nucleotide sequence