Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20855 and BPHYT_RS20860 are separated by 204 nucleotides BPHYT_RS20860 and BPHYT_RS20865 are separated by 33 nucleotides BPHYT_RS20865 and BPHYT_RS20870 are separated by 688 nucleotides
BPHYT_RS20855: BPHYT_RS20855 - transcriptional regulator, at 241,250 to 242,158
_RS20855
BPHYT_RS20860: BPHYT_RS20860 - hypothetical protein, at 242,363 to 242,686
_RS20860
BPHYT_RS20865: BPHYT_RS20865 - 2-hydroxyacid dehydrogenase, at 242,720 to 243,685
_RS20865
BPHYT_RS20870: BPHYT_RS20870 - cysteine hydrolase, at 244,374 to 244,943
_RS20870
Position (kb) 242
243
244 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 241.829 kb on - strand, within BPHYT_RS20855 at 241.953 kb on + strand, within BPHYT_RS20855 at 241.954 kb on - strand, within BPHYT_RS20855 at 242.060 kb on + strand, within BPHYT_RS20855 at 242.061 kb on - strand, within BPHYT_RS20855 at 242.128 kb on + strand at 242.229 kb on + strand at 242.785 kb on - strand at 243.424 kb on + strand, within BPHYT_RS20865 at 243.424 kb on + strand, within BPHYT_RS20865 at 243.916 kb on + strand at 244.052 kb on + strand at 244.375 kb on + strand at 244.444 kb on - strand, within BPHYT_RS20870 at 244.475 kb on - strand, within BPHYT_RS20870 at 244.475 kb on - strand, within BPHYT_RS20870
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 241,829 - BPHYT_RS20855 0.64 -0.9 241,953 + BPHYT_RS20855 0.77 -0.9 241,954 - BPHYT_RS20855 0.77 +2.4 242,060 + BPHYT_RS20855 0.89 -0.8 242,061 - BPHYT_RS20855 0.89 +0.4 242,128 + -1.6 242,229 + +1.0 242,785 - -1.3 243,424 + BPHYT_RS20865 0.73 -3.8 243,424 + BPHYT_RS20865 0.73 -0.5 243,916 + -0.5 244,052 + +0.1 244,375 + -0.6 244,444 - BPHYT_RS20870 0.12 +0.6 244,475 - BPHYT_RS20870 0.18 +0.1 244,475 - BPHYT_RS20870 0.18 +0.5
Or see this region's nucleotide sequence