Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_25320

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_25310 and AO353_25315 are separated by 64 nucleotidesAO353_25315 and AO353_25320 overlap by 4 nucleotidesAO353_25320 and AO353_25325 overlap by 7 nucleotidesAO353_25325 and AO353_25330 overlap by 4 nucleotides AO353_25310: AO353_25310 - malonate carrier protein, at 5,584,023 to 5,584,439 _25310 AO353_25315: AO353_25315 - malonate decarboxylase subunit epsilon, at 5,584,504 to 5,585,424 _25315 AO353_25320: AO353_25320 - phosphoribosyl-dephospho-CoA transferase, at 5,585,421 to 5,586,041 _25320 AO353_25325: AO353_25325 - biotin-independent malonate decarboxylase subunit gamma, at 5,586,035 to 5,586,838 _25325 AO353_25330: AO353_25330 - biotin-independent malonate decarboxylase subunit beta, at 5,586,835 to 5,587,686 _25330 Position (kb) 5585 5586 5587Strain fitness (log2 ratio) -2 -1 0 1 2at 5584.429 kb on + strandat 5584.429 kb on + strandat 5584.434 kb on - strandat 5584.434 kb on - strandat 5584.434 kb on - strandat 5584.434 kb on - strandat 5584.471 kb on + strandat 5584.471 kb on + strandat 5584.472 kb on - strandat 5584.675 kb on + strand, within AO353_25315at 5584.826 kb on - strand, within AO353_25315at 5585.199 kb on - strand, within AO353_25315at 5585.208 kb on + strand, within AO353_25315at 5585.208 kb on + strand, within AO353_25315at 5585.208 kb on + strand, within AO353_25315at 5585.208 kb on + strand, within AO353_25315at 5585.208 kb on + strand, within AO353_25315at 5585.209 kb on - strand, within AO353_25315at 5585.209 kb on - strand, within AO353_25315at 5585.209 kb on - strand, within AO353_25315at 5585.209 kb on - strand, within AO353_25315at 5585.209 kb on - strand, within AO353_25315at 5585.308 kb on + strand, within AO353_25315at 5585.858 kb on - strand, within AO353_25320at 5585.858 kb on - strand, within AO353_25320at 5585.858 kb on - strand, within AO353_25320at 5585.883 kb on + strand, within AO353_25320at 5585.883 kb on + strand, within AO353_25320at 5585.883 kb on + strand, within AO353_25320at 5585.884 kb on - strand, within AO353_25320at 5585.884 kb on - strand, within AO353_25320at 5586.150 kb on - strand, within AO353_25325at 5586.240 kb on + strand, within AO353_25325at 5586.240 kb on + strand, within AO353_25325at 5586.240 kb on + strand, within AO353_25325at 5586.240 kb on + strand, within AO353_25325at 5586.241 kb on - strand, within AO353_25325at 5586.241 kb on - strand, within AO353_25325at 5586.241 kb on - strand, within AO353_25325at 5586.241 kb on - strand, within AO353_25325at 5586.241 kb on - strand, within AO353_25325at 5586.241 kb on - strand, within AO353_25325at 5586.241 kb on - strand, within AO353_25325at 5586.241 kb on - strandat 5586.255 kb on + strand, within AO353_25325at 5586.256 kb on - strand, within AO353_25325at 5586.477 kb on + strand, within AO353_25325at 5586.477 kb on + strand, within AO353_25325at 5586.477 kb on + strand, within AO353_25325at 5586.477 kb on + strand, within AO353_25325at 5586.477 kb on + strand, within AO353_25325at 5586.477 kb on + strand, within AO353_25325at 5586.478 kb on - strand, within AO353_25325at 5586.478 kb on - strand, within AO353_25325at 5586.829 kb on - strandat 5586.829 kb on - strandat 5587.016 kb on + strand, within AO353_25330at 5587.016 kb on + strand, within AO353_25330

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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5,584,429 + -0.5
5,584,429 + -0.9
5,584,434 - -0.7
5,584,434 - -0.7
5,584,434 - -0.2
5,584,434 - -0.6
5,584,471 + -0.6
5,584,471 + +1.1
5,584,472 - +1.1
5,584,675 + AO353_25315 0.19 -1.9
5,584,826 - AO353_25315 0.35 +2.1
5,585,199 - AO353_25315 0.75 +1.5
5,585,208 + AO353_25315 0.76 +0.5
5,585,208 + AO353_25315 0.76 -0.0
5,585,208 + AO353_25315 0.76 -1.1
5,585,208 + AO353_25315 0.76 +0.4
5,585,208 + AO353_25315 0.76 -0.2
5,585,209 - AO353_25315 0.77 -0.9
5,585,209 - AO353_25315 0.77 -0.5
5,585,209 - AO353_25315 0.77 -0.1
5,585,209 - AO353_25315 0.77 +0.4
5,585,209 - AO353_25315 0.77 -0.5
5,585,308 + AO353_25315 0.87 -1.9
5,585,858 - AO353_25320 0.70 -0.1
5,585,858 - AO353_25320 0.70 -1.0
5,585,858 - AO353_25320 0.70 -1.9
5,585,883 + AO353_25320 0.74 -2.2
5,585,883 + AO353_25320 0.74 -0.7
5,585,883 + AO353_25320 0.74 -0.1
5,585,884 - AO353_25320 0.75 -0.5
5,585,884 - AO353_25320 0.75 -0.9
5,586,150 - AO353_25325 0.14 -0.0
5,586,240 + AO353_25325 0.25 +0.4
5,586,240 + AO353_25325 0.25 -0.9
5,586,240 + AO353_25325 0.25 +0.3
5,586,240 + AO353_25325 0.25 -0.7
5,586,241 - AO353_25325 0.26 +0.6
5,586,241 - AO353_25325 0.26 -0.5
5,586,241 - AO353_25325 0.26 +0.9
5,586,241 - AO353_25325 0.26 +0.5
5,586,241 - AO353_25325 0.26 +0.1
5,586,241 - AO353_25325 0.26 -0.5
5,586,241 - AO353_25325 0.26 +0.4
5,586,241 - +1.7
5,586,255 + AO353_25325 0.27 -2.0
5,586,256 - AO353_25325 0.27 -1.2
5,586,477 + AO353_25325 0.55 -0.4
5,586,477 + AO353_25325 0.55 -0.6
5,586,477 + AO353_25325 0.55 +0.8
5,586,477 + AO353_25325 0.55 -0.4
5,586,477 + AO353_25325 0.55 +0.3
5,586,477 + AO353_25325 0.55 -0.6
5,586,478 - AO353_25325 0.55 +1.6
5,586,478 - AO353_25325 0.55 -0.1
5,586,829 - +0.1
5,586,829 - -1.2
5,587,016 + AO353_25330 0.21 -0.0
5,587,016 + AO353_25330 0.21 -0.0

Or see this region's nucleotide sequence