Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS19190 and ABZR86_RS19195 overlap by 8 nucleotides ABZR86_RS19195 and ABZR86_RS19200 are separated by 12 nucleotides ABZR86_RS19200 and ABZR86_RS19205 are separated by 336 nucleotides
ABZR86_RS19190: ABZR86_RS19190 - efflux RND transporter permease subunit, at 290,063 to 293,209
_RS19190
ABZR86_RS19195: ABZR86_RS19195 - efflux transporter outer membrane subunit, at 293,202 to 294,617
_RS19195
ABZR86_RS19200: ABZR86_RS19200 - TetR/AcrR family transcriptional regulator, at 294,630 to 295,253
_RS19200
ABZR86_RS19205: ABZR86_RS19205 - low-specificity L-threonine aldolase, at 295,590 to 296,612
_RS19205
Position (kb) 293
294
295 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 292.247 kb on + strand, within ABZR86_RS19190 at 292.322 kb on + strand, within ABZR86_RS19190 at 292.322 kb on + strand, within ABZR86_RS19190 at 292.323 kb on - strand, within ABZR86_RS19190 at 292.323 kb on - strand, within ABZR86_RS19190 at 292.323 kb on - strand, within ABZR86_RS19190 at 292.505 kb on + strand, within ABZR86_RS19190 at 292.640 kb on + strand, within ABZR86_RS19190 at 292.640 kb on + strand, within ABZR86_RS19190 at 292.679 kb on + strand, within ABZR86_RS19190 at 292.680 kb on - strand, within ABZR86_RS19190 at 292.680 kb on - strand, within ABZR86_RS19190 at 292.680 kb on - strand, within ABZR86_RS19190 at 292.680 kb on - strand, within ABZR86_RS19190 at 292.680 kb on - strand, within ABZR86_RS19190 at 292.724 kb on + strand, within ABZR86_RS19190 at 292.826 kb on + strand, within ABZR86_RS19190 at 292.827 kb on - strand, within ABZR86_RS19190 at 292.827 kb on - strand, within ABZR86_RS19190 at 292.827 kb on - strand, within ABZR86_RS19190 at 293.207 kb on + strand at 293.207 kb on + strand at 293.208 kb on - strand at 293.208 kb on - strand at 293.388 kb on + strand, within ABZR86_RS19195 at 293.388 kb on + strand, within ABZR86_RS19195 at 293.389 kb on - strand, within ABZR86_RS19195 at 293.389 kb on - strand, within ABZR86_RS19195 at 293.389 kb on - strand, within ABZR86_RS19195 at 293.389 kb on - strand, within ABZR86_RS19195 at 293.589 kb on + strand, within ABZR86_RS19195 at 293.589 kb on + strand, within ABZR86_RS19195 at 293.913 kb on + strand, within ABZR86_RS19195 at 293.914 kb on - strand, within ABZR86_RS19195 at 294.322 kb on - strand, within ABZR86_RS19195 at 294.460 kb on - strand, within ABZR86_RS19195 at 294.460 kb on - strand, within ABZR86_RS19195 at 294.460 kb on - strand, within ABZR86_RS19195 at 294.480 kb on + strand at 294.480 kb on + strand at 294.480 kb on + strand at 294.480 kb on + strand at 294.480 kb on + strand at 294.480 kb on + strand at 294.481 kb on - strand at 294.481 kb on - strand at 294.481 kb on - strand at 294.481 kb on - strand at 294.481 kb on - strand at 294.513 kb on + strand at 294.513 kb on + strand at 294.514 kb on - strand at 294.533 kb on + strand at 294.533 kb on + strand at 294.576 kb on + strand at 294.577 kb on - strand at 294.577 kb on - strand at 294.577 kb on - strand at 294.578 kb on + strand at 294.578 kb on + strand at 294.578 kb on + strand at 294.578 kb on + strand at 294.795 kb on + strand, within ABZR86_RS19200 at 294.795 kb on + strand, within ABZR86_RS19200 at 294.796 kb on - strand, within ABZR86_RS19200 at 294.796 kb on - strand, within ABZR86_RS19200 at 294.801 kb on + strand, within ABZR86_RS19200 at 294.802 kb on - strand, within ABZR86_RS19200 at 294.812 kb on + strand, within ABZR86_RS19200 at 294.813 kb on - strand, within ABZR86_RS19200 at 295.197 kb on - strand at 295.236 kb on + strand at 295.257 kb on - strand at 295.257 kb on - strand at 295.257 kb on - strand at 295.257 kb on - strand at 295.287 kb on + strand at 295.287 kb on + strand at 295.457 kb on - strand at 295.457 kb on - strand at 295.457 kb on - strand at 295.517 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 292,247 + ABZR86_RS19190 0.69 -3.8 292,322 + ABZR86_RS19190 0.72 -0.1 292,322 + ABZR86_RS19190 0.72 -2.9 292,323 - ABZR86_RS19190 0.72 -3.7 292,323 - ABZR86_RS19190 0.72 -2.3 292,323 - ABZR86_RS19190 0.72 -3.8 292,505 + ABZR86_RS19190 0.78 -3.2 292,640 + ABZR86_RS19190 0.82 -3.0 292,640 + ABZR86_RS19190 0.82 -3.8 292,679 + ABZR86_RS19190 0.83 -0.7 292,680 - ABZR86_RS19190 0.83 -2.1 292,680 - ABZR86_RS19190 0.83 -3.0 292,680 - ABZR86_RS19190 0.83 +0.5 292,680 - ABZR86_RS19190 0.83 -2.8 292,680 - ABZR86_RS19190 0.83 -2.9 292,724 + ABZR86_RS19190 0.85 -4.2 292,826 + ABZR86_RS19190 0.88 -3.4 292,827 - ABZR86_RS19190 0.88 -4.7 292,827 - ABZR86_RS19190 0.88 -2.6 292,827 - ABZR86_RS19190 0.88 -3.0 293,207 + -1.1 293,207 + -2.1 293,208 - -1.9 293,208 - -1.5 293,388 + ABZR86_RS19195 0.13 -2.5 293,388 + ABZR86_RS19195 0.13 -2.1 293,389 - ABZR86_RS19195 0.13 -1.4 293,389 - ABZR86_RS19195 0.13 -2.2 293,389 - ABZR86_RS19195 0.13 -3.7 293,389 - ABZR86_RS19195 0.13 -1.3 293,589 + ABZR86_RS19195 0.27 -1.2 293,589 + ABZR86_RS19195 0.27 -3.8 293,913 + ABZR86_RS19195 0.50 -1.6 293,914 - ABZR86_RS19195 0.50 -0.9 294,322 - ABZR86_RS19195 0.79 -0.8 294,460 - ABZR86_RS19195 0.89 -1.5 294,460 - ABZR86_RS19195 0.89 -1.7 294,460 - ABZR86_RS19195 0.89 -3.6 294,480 + -2.7 294,480 + -2.1 294,480 + -1.7 294,480 + -4.0 294,480 + -3.1 294,480 + -1.7 294,481 - -1.8 294,481 - -1.2 294,481 - -1.8 294,481 - -0.8 294,481 - -0.6 294,513 + -4.7 294,513 + -2.8 294,514 - -1.4 294,533 + -2.0 294,533 + -0.7 294,576 + -2.8 294,577 - -2.7 294,577 - -0.5 294,577 - -3.0 294,578 + -4.2 294,578 + -4.1 294,578 + -3.0 294,578 + -3.9 294,795 + ABZR86_RS19200 0.26 +6.3 294,795 + ABZR86_RS19200 0.26 +6.6 294,796 - ABZR86_RS19200 0.27 +6.0 294,796 - ABZR86_RS19200 0.27 +5.7 294,801 + ABZR86_RS19200 0.27 +6.1 294,802 - ABZR86_RS19200 0.28 +5.9 294,812 + ABZR86_RS19200 0.29 +6.6 294,813 - ABZR86_RS19200 0.29 +5.8 295,197 - +6.4 295,236 + +6.2 295,257 - -0.0 295,257 - +0.5 295,257 - -0.7 295,257 - +0.5 295,287 + -1.0 295,287 + +0.1 295,457 - +0.1 295,457 - -1.5 295,457 - -1.0 295,517 - +0.3
Or see this region's nucleotide sequence