Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS15330 and ABZR86_RS15335 are separated by 102 nucleotides ABZR86_RS15335 and ABZR86_RS15340 overlap by 4 nucleotides ABZR86_RS15340 and ABZR86_RS15345 are separated by 6 nucleotides ABZR86_RS15345 and ABZR86_RS15350 are separated by 4 nucleotides
ABZR86_RS15330: ABZR86_RS15330 - TetR/AcrR family transcriptional regulator, at 3,451,415 to 3,452,032
_RS15330
ABZR86_RS15335: ABZR86_RS15335 - lysophospholipid acyltransferase family protein, at 3,452,135 to 3,452,935
_RS15335
ABZR86_RS15340: ABZR86_RS15340 - alpha/beta fold hydrolase, at 3,452,932 to 3,453,933
_RS15340
ABZR86_RS15345: ABZR86_RS15345 - 2OG-Fe(II) oxygenase, at 3,453,940 to 3,454,572
_RS15345
ABZR86_RS15350: ABZR86_RS15350 - AAA family ATPase, at 3,454,577 to 3,456,052
_RS15350
Position (kb)
3452
3453
3454 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.937 kb on + strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3451.938 kb on - strand, within ABZR86_RS15330 at 3452.102 kb on + strand at 3452.102 kb on + strand at 3452.103 kb on - strand at 3452.103 kb on - strand at 3452.103 kb on - strand at 3452.103 kb on - strand at 3452.113 kb on + strand at 3452.124 kb on + strand at 3452.189 kb on + strand at 3452.190 kb on - strand at 3452.221 kb on + strand, within ABZR86_RS15335 at 3452.222 kb on - strand, within ABZR86_RS15335 at 3452.222 kb on - strand, within ABZR86_RS15335 at 3452.222 kb on - strand, within ABZR86_RS15335 at 3452.222 kb on - strand, within ABZR86_RS15335 at 3452.222 kb on - strand, within ABZR86_RS15335 at 3452.222 kb on - strand, within ABZR86_RS15335 at 3452.222 kb on - strand, within ABZR86_RS15335 at 3452.642 kb on - strand, within ABZR86_RS15335 at 3453.196 kb on + strand, within ABZR86_RS15340 at 3453.196 kb on + strand, within ABZR86_RS15340 at 3453.196 kb on + strand, within ABZR86_RS15340 at 3453.196 kb on + strand, within ABZR86_RS15340 at 3453.196 kb on + strand, within ABZR86_RS15340 at 3453.197 kb on - strand, within ABZR86_RS15340 at 3453.197 kb on - strand, within ABZR86_RS15340 at 3453.197 kb on - strand, within ABZR86_RS15340 at 3453.197 kb on - strand, within ABZR86_RS15340 at 3453.197 kb on - strand, within ABZR86_RS15340 at 3453.197 kb on - strand, within ABZR86_RS15340 at 3453.197 kb on - strand, within ABZR86_RS15340 at 3453.197 kb on - strand, within ABZR86_RS15340 at 3453.454 kb on + strand, within ABZR86_RS15340 at 3453.454 kb on + strand, within ABZR86_RS15340 at 3453.454 kb on + strand, within ABZR86_RS15340 at 3453.529 kb on + strand, within ABZR86_RS15340 at 3453.530 kb on - strand, within ABZR86_RS15340 at 3453.538 kb on + strand, within ABZR86_RS15340 at 3453.538 kb on + strand, within ABZR86_RS15340 at 3453.601 kb on + strand, within ABZR86_RS15340 at 3453.692 kb on - strand, within ABZR86_RS15340 at 3453.692 kb on - strand, within ABZR86_RS15340 at 3453.898 kb on + strand at 3453.899 kb on - strand at 3454.097 kb on + strand, within ABZR86_RS15345 at 3454.098 kb on - strand, within ABZR86_RS15345 at 3454.098 kb on - strand, within ABZR86_RS15345 at 3454.098 kb on - strand, within ABZR86_RS15345 at 3454.145 kb on + strand, within ABZR86_RS15345 at 3454.148 kb on + strand, within ABZR86_RS15345 at 3454.148 kb on + strand, within ABZR86_RS15345 at 3454.149 kb on - strand, within ABZR86_RS15345 at 3454.205 kb on + strand, within ABZR86_RS15345 at 3454.223 kb on + strand, within ABZR86_RS15345 at 3454.311 kb on - strand, within ABZR86_RS15345 at 3454.311 kb on - strand, within ABZR86_RS15345 at 3454.311 kb on - strand, within ABZR86_RS15345 at 3454.311 kb on - strand, within ABZR86_RS15345 at 3454.311 kb on - strand, within ABZR86_RS15345 at 3454.722 kb on + strand at 3454.722 kb on + strand at 3454.722 kb on + strand at 3454.723 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 3,451,937 + ABZR86_RS15330 0.84 -4.3 3,451,937 + ABZR86_RS15330 0.84 -2.8 3,451,937 + ABZR86_RS15330 0.84 +0.1 3,451,937 + ABZR86_RS15330 0.84 -3.6 3,451,937 + ABZR86_RS15330 0.84 -3.2 3,451,937 + ABZR86_RS15330 0.84 -3.6 3,451,937 + ABZR86_RS15330 0.84 -2.6 3,451,937 + ABZR86_RS15330 0.84 -3.3 3,451,937 + ABZR86_RS15330 0.84 -0.7 3,451,938 - ABZR86_RS15330 0.85 -2.1 3,451,938 - ABZR86_RS15330 0.85 -2.3 3,451,938 - ABZR86_RS15330 0.85 -3.7 3,451,938 - ABZR86_RS15330 0.85 -1.4 3,451,938 - ABZR86_RS15330 0.85 -3.8 3,451,938 - ABZR86_RS15330 0.85 -2.9 3,451,938 - ABZR86_RS15330 0.85 -3.1 3,451,938 - ABZR86_RS15330 0.85 -2.5 3,451,938 - ABZR86_RS15330 0.85 -2.8 3,452,102 + -0.0 3,452,102 + +1.2 3,452,103 - -0.7 3,452,103 - -0.1 3,452,103 - +0.2 3,452,103 - -0.5 3,452,113 + +0.5 3,452,124 + +0.8 3,452,189 + -2.5 3,452,190 - -1.5 3,452,221 + ABZR86_RS15335 0.11 -0.6 3,452,222 - ABZR86_RS15335 0.11 -2.2 3,452,222 - ABZR86_RS15335 0.11 -1.8 3,452,222 - ABZR86_RS15335 0.11 -1.3 3,452,222 - ABZR86_RS15335 0.11 -2.2 3,452,222 - ABZR86_RS15335 0.11 -0.0 3,452,222 - ABZR86_RS15335 0.11 -1.6 3,452,222 - ABZR86_RS15335 0.11 -1.1 3,452,642 - ABZR86_RS15335 0.63 -2.1 3,453,196 + ABZR86_RS15340 0.26 -1.4 3,453,196 + ABZR86_RS15340 0.26 -1.6 3,453,196 + ABZR86_RS15340 0.26 -1.2 3,453,196 + ABZR86_RS15340 0.26 -1.0 3,453,196 + ABZR86_RS15340 0.26 -2.1 3,453,197 - ABZR86_RS15340 0.26 -0.9 3,453,197 - ABZR86_RS15340 0.26 -0.9 3,453,197 - ABZR86_RS15340 0.26 -1.7 3,453,197 - ABZR86_RS15340 0.26 -1.7 3,453,197 - ABZR86_RS15340 0.26 -2.6 3,453,197 - ABZR86_RS15340 0.26 -1.1 3,453,197 - ABZR86_RS15340 0.26 -0.7 3,453,197 - ABZR86_RS15340 0.26 -1.4 3,453,454 + ABZR86_RS15340 0.52 -1.5 3,453,454 + ABZR86_RS15340 0.52 -2.0 3,453,454 + ABZR86_RS15340 0.52 -1.4 3,453,529 + ABZR86_RS15340 0.60 -1.9 3,453,530 - ABZR86_RS15340 0.60 -2.9 3,453,538 + ABZR86_RS15340 0.60 -3.7 3,453,538 + ABZR86_RS15340 0.60 -0.8 3,453,601 + ABZR86_RS15340 0.67 -1.2 3,453,692 - ABZR86_RS15340 0.76 -1.5 3,453,692 - ABZR86_RS15340 0.76 -1.2 3,453,898 + -1.5 3,453,899 - -2.5 3,454,097 + ABZR86_RS15345 0.25 -0.6 3,454,098 - ABZR86_RS15345 0.25 -1.4 3,454,098 - ABZR86_RS15345 0.25 -1.0 3,454,098 - ABZR86_RS15345 0.25 +0.2 3,454,145 + ABZR86_RS15345 0.32 -0.6 3,454,148 + ABZR86_RS15345 0.33 -0.9 3,454,148 + ABZR86_RS15345 0.33 -0.8 3,454,149 - ABZR86_RS15345 0.33 -1.0 3,454,205 + ABZR86_RS15345 0.42 -0.8 3,454,223 + ABZR86_RS15345 0.45 -0.8 3,454,311 - ABZR86_RS15345 0.59 -1.5 3,454,311 - ABZR86_RS15345 0.59 -1.6 3,454,311 - ABZR86_RS15345 0.59 -0.5 3,454,311 - ABZR86_RS15345 0.59 +0.1 3,454,311 - ABZR86_RS15345 0.59 -1.3 3,454,722 + -0.3 3,454,722 + -1.0 3,454,722 + -1.3 3,454,723 - +1.4
Or see this region's nucleotide sequence