Experiment: Sisomicin 0.016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS12800 and ABZR86_RS12805 are separated by 55 nucleotides ABZR86_RS12805 and ABZR86_RS12810 are separated by 196 nucleotides ABZR86_RS12810 and ABZR86_RS12815 are separated by 13 nucleotides ABZR86_RS12815 and ABZR86_RS12820 are separated by 138 nucleotides ABZR86_RS12820 and ABZR86_RS12825 overlap by 4 nucleotides
ABZR86_RS12800: ABZR86_RS12800 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 2,869,797 to 2,870,846
_RS12800
ABZR86_RS12805: ABZR86_RS12805 - 30S ribosomal protein S21, at 2,870,902 to 2,871,117
_RS12805
ABZR86_RS12810: ABZR86_RS12810 - GatB/YqeY domain-containing protein, at 2,871,314 to 2,871,760
_RS12810
ABZR86_RS12815: ABZR86_RS12815 - hypothetical protein, at 2,871,774 to 2,872,094
_RS12815
ABZR86_RS12820: ABZR86_RS12820 - DUF1328 domain-containing protein, at 2,872,233 to 2,872,403
_RS12820
ABZR86_RS12825: ABZR86_RS12825 - N-acetyltransferase, at 2,872,400 to 2,872,978
_RS12825
Position (kb)
2871
2872 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2871.102 kb on + strand at 2871.112 kb on + strand at 2871.178 kb on + strand at 2871.185 kb on + strand at 2871.186 kb on - strand at 2871.272 kb on + strand at 2871.273 kb on - strand at 2871.512 kb on + strand, within ABZR86_RS12810 at 2871.513 kb on - strand, within ABZR86_RS12810 at 2871.578 kb on + strand, within ABZR86_RS12810 at 2871.579 kb on - strand, within ABZR86_RS12810 at 2871.579 kb on - strand, within ABZR86_RS12810 at 2871.579 kb on - strand, within ABZR86_RS12810 at 2871.919 kb on + strand, within ABZR86_RS12815 at 2871.919 kb on + strand, within ABZR86_RS12815 at 2871.919 kb on + strand, within ABZR86_RS12815 at 2871.920 kb on - strand, within ABZR86_RS12815 at 2871.920 kb on - strand, within ABZR86_RS12815 at 2871.967 kb on + strand, within ABZR86_RS12815 at 2871.967 kb on + strand, within ABZR86_RS12815 at 2872.022 kb on + strand, within ABZR86_RS12815 at 2872.110 kb on + strand at 2872.111 kb on - strand at 2872.111 kb on - strand at 2872.111 kb on - strand at 2872.111 kb on - strand at 2872.215 kb on + strand at 2872.216 kb on - strand at 2872.221 kb on - strand at 2872.293 kb on + strand, within ABZR86_RS12820 at 2872.294 kb on - strand, within ABZR86_RS12820 at 2872.501 kb on - strand, within ABZR86_RS12825 at 2872.650 kb on + strand, within ABZR86_RS12825 at 2872.650 kb on + strand, within ABZR86_RS12825 at 2872.650 kb on + strand, within ABZR86_RS12825 at 2872.650 kb on + strand, within ABZR86_RS12825 at 2872.651 kb on - strand, within ABZR86_RS12825 at 2872.651 kb on - strand, within ABZR86_RS12825
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.016 mg/ml remove 2,871,102 + +0.2 2,871,112 + -0.4 2,871,178 + -1.0 2,871,185 + -0.4 2,871,186 - +0.2 2,871,272 + +2.1 2,871,273 - +0.1 2,871,512 + ABZR86_RS12810 0.44 +0.1 2,871,513 - ABZR86_RS12810 0.45 +0.5 2,871,578 + ABZR86_RS12810 0.59 -0.1 2,871,579 - ABZR86_RS12810 0.59 +0.1 2,871,579 - ABZR86_RS12810 0.59 +0.5 2,871,579 - ABZR86_RS12810 0.59 +1.1 2,871,919 + ABZR86_RS12815 0.45 +0.1 2,871,919 + ABZR86_RS12815 0.45 -0.1 2,871,919 + ABZR86_RS12815 0.45 -0.2 2,871,920 - ABZR86_RS12815 0.45 -0.1 2,871,920 - ABZR86_RS12815 0.45 +0.5 2,871,967 + ABZR86_RS12815 0.60 -0.4 2,871,967 + ABZR86_RS12815 0.60 -0.1 2,872,022 + ABZR86_RS12815 0.77 -0.7 2,872,110 + -0.6 2,872,111 - +0.9 2,872,111 - +0.5 2,872,111 - +0.8 2,872,111 - +1.4 2,872,215 + +0.7 2,872,216 - -1.9 2,872,221 - -0.1 2,872,293 + ABZR86_RS12820 0.35 +0.1 2,872,294 - ABZR86_RS12820 0.36 -0.0 2,872,501 - ABZR86_RS12825 0.17 +0.3 2,872,650 + ABZR86_RS12825 0.43 +1.0 2,872,650 + ABZR86_RS12825 0.43 -0.2 2,872,650 + ABZR86_RS12825 0.43 +0.1 2,872,650 + ABZR86_RS12825 0.43 -0.2 2,872,651 - ABZR86_RS12825 0.43 -0.3 2,872,651 - ABZR86_RS12825 0.43 +1.2
Or see this region's nucleotide sequence